水飞蓟素提取工艺的改进和探讨

:2006-02-20:3(BE2005338)1,1,1,2(11,210042;21,210095),710%,(10h),3,9710%,7107mg/g,;;:R284.2:A:1006-9690(2006)06-0052-03ImprovementandApproachontheExtrationofSilymarinShiJinsong1,SunDafeng1,GuGongping1,XuDefeng2(11NanjingInstituteforComprehensiveUtilizationofWildPlants,Nanjing210042,China;21CollegeofFoodScienceandTechnologyofNanjingAgriculturalUniversity,Nanjing210095,China)AbstractPreliminarystudieswerecarriedoutontheextractionofsilymarinfromthesegregatedpeelofSily2bummarianumGaertnfruit1Theresultsshowthatsilymarinover710%yieldoffeedstockcanbeobtainedwithaceticetherextractionassistedbyultrasonic,andthattheextractiontimecanbeshortenedtotenhours1TheSilybincanbepurifiedto9710%afterthreetimesrecrystallizationinmethanol,itstotalyieldoffeedstockis7107mg/g1Itwassuggestedthatthesilybumkernalandpeelshouldbeseparatedbeforeoilcrushingandsily2marinextracting1Thisprocessingcouldsignificantlyimprovetheproductqualityandenhancetheutilizationval2ueofSilybummarianumresource1Keywordssilymarin;silibin;ultrasonicassistantextraction(SilybummarianumL.Gaertn),,[1],,(silymarin),(silybin),[2],,,[3-5]2070,,,300t,,70%80%,,,,11.1,,,,,,40(99%,HPLC),25256200612ChineseWildPlantResourcesVol.25No.6Dec.20061.2(TG328A)(20PR-52D)(KQ-250)(SP-752)1.3:20g,120mL8h,3,,,,:,50,4h,2:,20g120mL8h,:,50,4h,2,:,,1143,12(gmL)15min,2d,10min(8000r/min),3,3,2,,115(9910%)2112mg,80%,100mL,011,0125,0150,0175,110mL,80%10mL,288nmA,C(mg/mL)C=010229A-01002,R2=019984:0150g,100mL,3h,,,,1mL10UV,:2030mg,100mL,,,,UV(35),,UV,UV[6],UV9815%,RSD015%018%,RSD117%210%22.1,4716%5214%,,,,,,,14716%7186%3168%5214%0132%4215%212,,,,4198%7179%7113%7159%,5%,7%,2141%,2158%,,,,,213,,2,,,;50,(5911mg/g),,,;356,:,,(7519mg/g),(78),,,,,,2//(w/v)/h/(mg/g)3/(mg/g)/(mg/g)20101:638349184318211850101:624259113610211378101:618175193511261650101:624270173615251831214,,,,(3),,,,,3Rf,,3(955,V/V)(9010,V/V)(955,V/V)(9010,V/V)/(mg/g))621585177412911610311(UV)9612%9218%9617%9414%9710%/(mg/g)412551625107519271073,,,710%,10h,9710%,7107mg/g,,,,,,;,,,,,,,,,,,,,,,810,,,[1],1I[J]1,l997,6(4):91-931[2],1[J]1,2005,27(1):19-221[3]VargaZ,SeresI,NagyEetal1Structureprerequisiteforantioxidantac2tivityofsilybinindifferentbiochemicalsystemsinvitro[J]1Phy2tomedicine2006,13:85-931[4]VladielaW1Silybinandsilymarin-neweffectsandapplications[J]1BiomedPapers,2005,149(1):29-41.[5]LeeDG,KimHK,ParkY,etal1Gram-positivebacteriaspecificpropertiesofsilybinderivedfromSilybummarianum[J]1ArchPharmRes,2003,26:597-6001[6],,1[J]1,2003(20),2:120-123,13114525