旱稻导入系碾磨品质和垩白粒率的QTL定位及其与土壤水分的互作分析_

作物学报ACTAAGRONOMICASINICA2009,35(5):831−838:[e-mail]本研究由国家重点基础研究发展计划(973计划)项目(2004CB117201),国家自然科学基金项目(30671281),国家高技术研究发展计划(863计划)项目(2006AA10Z158和2006AA100101)资助。*(Correspondingauthor):,E-mail:[e-mail];Tel:[phone]Received():2008-09-17;Accepted(:2008-12-13.DOI:10.3724/SP.J.1006.2009.00831旱稻导入系碾磨品质和垩白粒率的QTL定位及其与土壤水分的互作分析李俊周付春阳李自超*/,100193:,IRAT109271,24,,QTL,,,,410QTL2QTL,347893QTL(qMR9qHMR7qHMR9)QTL(qHMR3~qHMR9)10%7QTL4RM1112~RM12729RM1189~RM410QTL,QTLQTL,:;;;QTL;QTLMappingandQTL×EnvironmentInteractionsofMillingQualityandPercentageofChalkyGraininUplandRiceIntrogressionLinesunderUplandandLowlandEnvironmentsLIJun-Zhou,FUChun-Yang,andLIZi-Chao*KeyLaboratoryofCropGenomicsandGeneticImprovement,MinistryofAgriculture/BeijingKeyLaboratoryofCropGeneticImprovement,ChinaAgriculturalUniversity,Beijing100193,ChinaAbstract:Millingqualityandpercentageofchalkygrainofrice(OryzasativaL.)areimportanttraitstoproducersandconsumers.Theobjectivesofthisstudyweretocharacterizetheresponseofbrownrice(BR),milledrice(MR),headmilledrice(HMR),andpercentageofchalkygrain(PCG)touplandwaterstressandtoidentifyQTLsforthetraits.Asetof271introgressionlines(ILs)(BC5F3),carryingsegmentsfromuplandcultivarIRAT109inthebackgroundoflowlandricecultivarYuefu,wereused,anddatawerecollectedunderuplandandlowlandenvironments.Greatchangesweredetectedbetweenuplandandlowlandconditionsinheadmilledriceandpercentageofchalkygrain,butrelativestabilityinbrownriceandmilledrice.Headmilledriceincreasedandpercentageofchalkygrainsignificantlydecreasedunderuplandcondition.AtotaloftenadditiveQTLsandtwopairsofdigenicepistaticinteractionsQTLsforBR,MR,HMR,andPCGweredetectedonchromosomes3,4,7,8,and9.ThecontributionofsingleQTLrangedfrom1.81%to17.49%,threeQTLs(qMR9,qHMR7,qHMR9)andonepairsofdigenicepistaticinteractions(qHMR3−qHMR9)accountedforabove10%ofthephenotypicvariation.SixQTLswerenosignificantQTL×Environmentin-teractioneffects,sevenQTLswerethesamewiththosefrompreviousreports,andtwogenomicintervals,RM1112−RM1272onchromosome4andRM1189−RM410onchromosome9,wereclusterregionsoftheQTLs.Theseco-localizedQTLsandcommonQTLswillfacilitatemarker-assistedselectionforgrainqualitytraitsinricebreeding.Keywords:Rice;Millingquality;Percentageofchalkygrain;QTL×Environmentinteraction;Introgressionlines(ILs)83235,,3,,,×[1-3],,,,[4],,[5],,QTL,,DNA,QTL[6-11],,,QTL,;F2DHRIL,,[12],QTL;QTL;,QTL[13]QTL,,,QTL1材料与方法1.1IRAT109,271IRAT109,;,IRAT109,F1,,BC5F3,441177SSRIndel,271IRAT109Cornell[14-15](77%),211(23%),14.4cM75.3,3.3,85,622,68%33.3%,0.1%,24.2%,78.6%(213)5%96.7%2006,(39°N,116°E),,,2,3,12,25cm×8cm2~10cm,()1300mm,2~3,50mm,()500mm,,39%1.25,3,Septiningsih[8]Tan[6]1.3SPSSV11.0()Yang[16]QTLNetworkQTL,,QTL1000,10cM,1cM,QTL5:QTL8330.052结果与分析2.11,4,,IRAT109,IRAT109;2,;,,,46.61%~76.79%4.50%,,,78.78%,,,()2.22,23,0.66~0.86,3,,0.15,,,−0.14−0.17−0.15表1水、旱田栽培环境下稻米碾磨品质和垩白粒率在亲本和导入系群体中的表现Table1PhenotypicvaluesofmillingqualityandpercentageofchalkygrainbetweenparentsandintrogressionlinesunderlowlandanduplandcultivationsParentsILsTraitCultivationYuefuIRAT109±Mean±SDRangeCV(%)Lowland83.0582.0082.87±1.5174.76−85.931.82Brownrice(%)Upland80.6878.5879.60±1.0772.58−82.531.34Lowland75.2872.0673.35±1.7663.89−78.212.37Milledrice(%)Upland55.4071.7675.38±1.2868.87−78.161.69Lowland73.2966.2664.84±3.3743.61−76.534.69Headmilledrice(%)Upland50.1161.7568.27±3.1548.69−77.044.30Lowland28.0097.0038.49±13.9711.00−90.0036.31Percentageofchalkygrain(%)Upland4.0070.0017.78±14.011.00−96.0078.78表2稻米碾磨品质和垩白粒率等各参数间的相关系数Table2CorrelationcoefficientsbetweenparametersofmillingqualityandpercentageofchalkygrainLowlandUplandBrownriceMilledriceHeadmilledriceBrownriceMilledriceHeadmilledrice/Lowland/UplandBrownrice0.21**Milledrice0.86**0.86**0.25**Headmilledrice0.73**0.81**0.66**0.74**0.33**Percentageofchalkygrain0.15*0.090.07–0.14*–0.17**–0.15*0.00***0.050.01*and**representsignificanceatthe0.05and0.01probabilitylevels,respectively.83435,,,,,,,2.3QTLQTLNetwork,,10QTL2QTL,4QTL2QTL(341)892QTL(qBR8qBR9)qBR8−0.37,IRAT109,1.81%;qBR90.77,3.93%2QTL(qMR4qMR9),2QTLqMR40.59,4.38%;qMR90.92,11.11%表3271份旱稻导入系中控制糙米率、精米率、整精米率和垩白粒率的加性效应QTL及其环境互作效应Table3AdditiveeffectsandQTL×EnvironmentinteractioneffectsofQTLsforbrownrice(BR),milledrice(MR),headmilledrice(HMR),andpercentageofchalkygrain(PCG)in271uplandriceintrogressionlinesAE2)H2%3)TraitQTLInternalPositionAdditiveeffect1)AE12)AE22)H2(a)%3)H2(ae)%3)qBR8RM3459−RM21075.7–0.371.81Brownrice(%)qBR9RM1189−RM41060.00.773.93qMR4RM1112−RM1272128.90.594.38Milledrice(%)qMR9RM1189−RM41060.00.9211.11qHMR3RM1338−RM667640.36.398.28qHMR4RM1112−RM1272128.91.530.43–0.457.021.23qHMR7RM1306−RM420118.14.61–1.321.2817.340.15Headmilledrice(%)qHMR9RM1189−RM41062.48.22–0.860.8710.220.05qPCG3RM6832−RM351379.9–5.012.69–2.75.630.02Percentageofchalkygrain(%)qPCG9RM1189−RM41063.6–5.464.431)QTL;2)AE,E1,E2;3)H2%QTL,H2(a)%,H2(ae)%QTL1)ThepositivevalueindicatesthattheYuefuallelehasapositiveeffectonth