标书范本-国家自然科学基金

1C030316***********11000192024-23256666024-23256666200310B51———335100083****************A.B.C.D.E.F.H.A121C0303162A.B.B15.7220031200412A.B.***A.195912XXXB.B210102******47601()011A.B.C.A156A.B.C.B****11000101110001A.B.C.A024-23256666921XX()XX()XXXXX()928331111***210102*****253**11000101***210102******183**11000101***210102******182**11000101DNAPCR***210105******316**11000101***210102******171**11000101PCR***410305******451**11000101**11000101()11.2./BAB——25(——————————112——————()————2DNADNAPCRDNADNA208090DNA11893NucleatedRBC,NRBCNRBC11091105NRBC90DNANRBCNRBC1.2.CD71GPANRBC32CD45fluorescence-activatedcellsorting,FACSmagnetic-activatedcellsorting,MACS23.NRBCNRBC4.NRBC3NRBCNRBC(4)NRBCCD45FACSMACS80%87.5%1.NRBC2.PKU5100%0%HumanLeukocyteAntigen,HLAHLAA-Exon2,A-Exon3,B-Exon2,B-Exon3DQA-Exon2,DQA-Exon3PCRHLADNA1.LoYMD,WainscoatJS,GilmerMDG,etal.PrenatalsexdeterminationbyDNAamplificationfrommaternalperipheralblood.Lancet,1989,2:1363-13652.BianchiDW,KlingerKW,VadnaisTJM,etal.Developmentofamodelsystemtocomparecellseparationmethodsfortheisolationoffetalcellsfrommaternalblood.PrenatalDiagnosis,1996,16:289-2963.WachtelSS,etal.Chargeflowseparation:qyantificationofnucleatedredbloodcellsinmaternalbloodduringpregnancy.PrenatalDiagnosis,1998:18:455-4634.WangTR,ChenHP,LuY,etal.Yspecificsequencegeneanalysisofsinglefetalnucleatederythroblastsfromtheperipheralbloodofpregnantwomen.JTongjiMedUniv.2001;21(2):163-5.5.WatanabeA,SekizawaA,TaguchiA,etal.Prenataldiagnosisofornithinetranscarbamylasedeficiencybyusingasinglenucleatederythrocytefrommaternalblood.HumGenet1998102:611-51.******1.2.42’A-Exon2,A-Exon3,B-Exon2,B-Exon3DQA-Exon2,DQA-Exon3PAGEPCRdATP96122.154.5.61.2.73.*…………*········8::1.2.3.4.5.6.98631030011121.132.1233.(1)(2)(3)14