细胞培养及培养基选用事项

MEDIAHANDBOOK2005-0-invitroculturetissueculturecellcultureorganculture501885Roux1903Jolly1906Beebe1907Harrison19101912Carrel1924Maximow1923CarralEarle40,,50ParkeEagle60Earle1948EarleHank’s1949Hank’sEarleDulbecco1943L-1948SanfordL-1951Gey——Hela1961Hayflick25-1-50Ohashi,Ryo20012LBectonDickensonCellMab+10%+1F-681×107cells/ml2001Heine,Holger(UCS)2×107cells/ml2004Thomas1LrCHOMUC-1TCA(12)×107cells/mlXieLiangzhi20002L1.7×107cells/mL11221())2EPO345,12MEM199DMEMRPMI1640-2-1020FDACO2199612002123121,12-3-34111-5%212,,3BVitc1-10mg/100ml23(lamininIV)4,535—37,39→00-346pHO2CO2CO2pHpH7.2—7.4CO2pH5%CO2HEPES()10—50mmol/mlpHEarle’s5CO2Hanks’0.35g/LNaHCO35CO2CO2-4-795%81234pH51L-L-L-L-L-L-L-L-L-L-2ADEKB1B2B6B12CVAVDVEVK-5-34,260mOsm/kg−320mOsm/kgNa+K+K+Ca2+Mg2+1//23-6-123pHpH4567891011231640IMDM42-7-1MEMDMEMM19923——3LLLLNH4+L-pH-8-4pH56pHpHpHpHpHpHpHpHDMEM(HED)MEMpH1DMEM(HED)-9-2MEM78123A.NaHCO3-10-486B.GMPSOPGMPGMPC.0.1-700µm0.1µm9HEPAB12B0.250.501.02.04.08.0mg/ml342323564746-11-balancedsaltsolutionBSS1885SydneyRinger51Dulbecco,sD-PBS2Hanks,HBSS0.35mg/L3Earle,sEBSS2.2mg/L4PBSCa2+Mg2+1,DMEM2-5-30%11991950MorganBSS53199HBVero199——199HB199HB199a)199HB199HB19924858548989572-12-b)pH199HBpH34pHc)199HB199199HB1996.866.436.635.83d)199HB199HB1994.243.492BMEEagleBasalMediumEagle1955EagleBSS128MEMDMEMIMDM3MEMEagleMinimalEssentialMedium1959MEMEarle'sHanks'MEMMEM4DMEMDMEMDulbeccoEagle1000mg/L4500mg/LDNACHOCHOEPODMEM——DMEMHEDDMEMHEDCHO-13-DMEMHEDDMEM1a)DMEMHEDDMEM2DMEMHED28037.5x106ml60dHBsAg11281512DMEM3040430dHBsAg13245d60db)HBsAgDMEMDMEMHEDDMEM30HBsAg3.75µg/ml3.51µg/ml5IMDMIMDMIscove'sEagle6RPMI-1640Moore1967RoswellParkMemorialInstituteBSS21117Fischer’s8HamF10F1219631969HamF10F12CHO1DMEMCHO-C282003Vol.16No.6P.380-382-14-9DMEM/F12DMEMF121:1211050702a)b)-15-103VEROBHK21521FDA/CHOCHO6-16-