工业微生物遗传育种的研究进展-(1)

ModernFoodScienceandTechnologyVol.21No.3(85)Q813.5A:1007-2764(2005)03-0184-0641122(1.200234)(2.410128)ResearchAdvancesintheBreedingofIndustrialMicrobeNieMing1LiHuai-bo1WanJia-rong2ZhouChuan-yun21.CollegeofLifeEnvironmentScienceShanghaiNormalUniversityShanghai200234,China(2.FacultyofFoodScienceTechnology,HunanAgriculturalUniversityChangsha410128,China)Abstract:Thisarticlepresentsthehistoricalstatusofthebreedingofindustrialmicrobe.Thetechniqueandmechanismofthebreedingofindustrialmicrobeisintroducedandtheapplicationprospectisforecastedaswellinthepaper.Keywords:Industrialmicrobe;Breeding;Technique;Mechanism[1][2]1:2005-05-251929Flemirlg1~2U/ml90000U/ml20%35%99.9%90%[3]22.1184ModernFoodScienceandTechnologyVol.21No.3(85)[3]2.2[2]X--(EMSEINEUNMUDESMNNGNTG)[1][4][5]2.3[26]2.3.1[5]185ModernFoodScienceandTechnologyVol.21No.3(85)2.3.2DNA11[2]DNA2.3.3PEG[7][8]:[9][10].[11]2.4[2][12]2.5[13]DNA186ModernFoodScienceandTechnologyVol.21No.3(85)[14~17](1)()()NorthercDNA()NodPCRDNADNAmRNA31982PCR[1].[J].2003322158-161.[2]EF.[M]..1992.12-68.[3].M..:,1998,92[4],.2001.[5].M.:[6],.M1988[7],.[M].:,2003[8].[J].2002295-99.[9].[M].:,2002,202[10]KaoKN.Amethodforhigh-frequencyintergenericfusionofplantprotoplast[J].Planta1974115355-367.[11]HopwoodDAWrightHM.GeneticrecombinationthroughprotoplastfusioninStreptomyces[J].JGenMicrobiol1979111(1)137-143.[12]HopwoodDAWrightHM.Bacterialprotoplastfusion:recombinationinfusedprotoplastsofStreptomyces[J].MolGenGenet1978162307-317.[13].[J].[J].198222(3)248-256.[14]KimBKKangJHJinMetal.MycelialprotoplastisolationandregenerationofLentinuslepideus[J].LifeSci200066(14)1359-1367.[15].[M].:200155-59.[16].[M].:2002.[17].[M].:,ResearchAdvancesintheBreedingofIndustrialMicrobe,2000.125187