第一章-植物组织培养的基础理论与基本知识

技术官员村位于位于亚运城东部，主干道二以东石楼涌以西的地块，占地面积、m2，总建筑面积、m2，共包括地下室南区、地下室北区、地上部分1栋～12栋、服务中心、室外工程等多个单体工程。其中住宅面积m2，共12栋，1～7栋建筑层数为11层，8～12栋11层（局部复式顶层），首层局部架空，布置公建配套设施。integratedenergy,chemicalsandtextileYibincity,arethethreecorepillarsoftheindustry.In2014,thewuliangyebrandvalueto73.58billionyuan,thecity'sliquorindustrysliptostabilise.Promotingdeepdevelopmentofintegratedenergy,advancedequipmentmanufacturingindustry,changningdistrict,shalegasproductioncapacityreached277millioncubicmetres,builtthecountry'sfirstindependenthigh-yieldwellsandpipelinesinthefirstsection,theleadinfactoryproductionandsupplytothepopulation.2.1-3GDPgrowthfigure2.1-4Yibin,Yibincity,Yibincity,fiscalrevenuegrowth2.1.4topographyterrainoverallisSouthwest,North-EasternState.Lowmountainsandhillsinthecitylandscapeasthemainridge-and-Valley,pingbasmallfragmentednaturepictureforwaterandthesecondlandofthesevenhills.236metersto2000metersabovesealevelinthecity,lowmountain,46.6%hills45.3%,pingbaonly8.1%.2.1.5developmentofYibinlandscapesanddistinctivefeatureinthecenterofthecity,withlimitations,andspatialstructureoftypicalzonalgroup,2012-citiesinbuildingwithanareaofabout76.2km2.Fromcity-buildingsituation,oldtown-theSouthBankCenterconstructionislaggingbehind,disintegrationoftheoldcityisslow,optimizationandupgrading,qualitypublicserviceresourcesarestillheavilyconcentratedintheoldtowntogether.SouthbankCentrehasnotbeenformed,functionsoftheserviceareaspaceismissing.Meanwhile,peripheralgroupcentresservicewasweakandinadequateaccountingforcitydevelopment,suspiciouspatterncouldnotbeformed.Asregardstransport,withtheoutwardexpansionofcities,citieshavebeenexpanding,centripetalcitytrafficorganizationhasnotchanged,integratedenergy,chemicalsandtextileYibincity,arethethreecorepillarsoftheindustry.In2014,thewuliangyebrandvalueto73.58billionyuan,thecity'sliquorindustrysliptostabilise.Promotingdeepdevelopmentofintegratedenergy,advancedequipmentmanufacturingindustry,changningdistrict,shalegasproductioncapacityreached277millioncubicmetres,builtthecountry'sfirstindependenthigh-yieldwellsandpipelinesinthefirstsection,theleadinfactoryproductionandsupplytothepopulation.2.1-3GDPgrowthfigure2.1-4Yibin,Yibincity,Yibincity,fiscalrevenuegrowth2.1.4topographyterrainoverallisSouthwest,North-EasternState.Lowmountainsandhillsinthecitylandscapeasthemainridge-and-Valley,pingbasmallfragmentednaturepictureforwaterandthesecondlandofthesevenhills.236metersto2000metersabovesealevelinthecity,lowmountain,46.6%hills45.3%,pingbaonly8.1%.2.1.5developmentofYibinlandscapesanddistinctivefeatureinthecenterofthecity,withlimitations,andspatialstructureoftypicalzonalgroup,2012-citiesinbuildingwithanareaofabout76.2km2.Fromcity-buildingsituation,oldtown-theSouthBankCenterconstructionislaggingbehind,disintegrationoftheoldcityisslow,optimizationandupgrading,qualitypublicserviceresourcesarestillheavilyconcentratedintheoldtowntogether.SouthbankCentrehasnotbeenformed,functionsoftheserviceareaspaceismissing.Meanwhile,peripheralgroupcentresservicewasweakandinadequateaccountingforcitydevelopment,suspiciouspatterncouldnotbeformed.Asregardstransport,withtheoutwardexpansionofcities,citieshavebeenexpanding,centripetalcitytrafficorganizationhasnotchanged,第一章植物组织培养的基础理论与基本知识目的要求：(1)掌握组织培养的概念和类型；(2)掌握组织培养的特点；(3)了解组织培养发展史；(4)初步掌握组织培养在农业实践上的应用。第一节植物组织培养的基本概念及类型一、植物组织培养的概念植物组织培养（tissueculture）指在无菌条件下，将离体的植物器官（如根、茎、叶、花、果、茎尖等）、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）、细胞（如大孢子、小孢子及体细胞）以及原生质体，培养在人工控制的环境里使其再生形成完整的植株。亦可称为无菌培养、离体培养、试管培养。根据培养基的形态可分为固体培养和液体培养；液体培养又可分为悬浮培养、振荡培养、旋转培养等。外植体（explant）：在植物组织培养过程中，有植物体上切取的根、茎、叶、花、果实、种子等器官以及各种组织和细胞统称为外植体。愈伤组织：植物局部创伤后或在组织培养中，当已分化的细胞恢复了细胞分裂能力，而增殖形成的无组织结构、无器官分化的薄壁细胞团。在组织培养中，先由外植体增殖产生，在一定条件下，对它经过一段时间培养，可能从其再分化出具一定结构及功能的器官，如根、茎、胚状体或重新形成完整的植株。二、植物组织培养的类型1.植物培养植物培养是指幼小植株的培养。例如兰花，兰花及兰科植物种子的发育特殊，种子成熟之后没有胚乳，需要与某些真菌共生，由真菌提供营养才能萌发，在自然条件下，萌发率很低。早期通过人工接种真菌可使兰花种子萌发，但技术繁琐，难度较大，后来发现在人工培养基上，只要营养成分合适不需要真菌共生，兰花种子也能萌发。以蝴蝶兰为例，由一个好的荚果播种后两个月左右可产生数万个小圆球茎，经过一个月技术官员村位于位于亚运城东部，主干道二以东石楼涌以西的地块，占地面积、m2，总建筑面积、m2，共包括地下室南区、地下室北区、地上部分1栋～12栋、服务中心、室外工程等多个单体工程。其中住宅面积m2，共12栋，1～7栋建筑层数为11层，8～12栋11层（局部复式顶层），首层局部架空，布置公建配套设施。integratedenergy,chemicalsandtextileYibincity,arethethreecorepillarsoftheindustry.In2014,thewuliangyebrandvalueto73.58billionyuan,thecity'sliquorindustrysliptostabilise.Promotingdeepdevelopmentofintegratedenergy,advancedequipmentmanufacturingindustry,changningdistrict,shalegasproductioncapacityreached277millioncubicmetres,builtthecountry'sfirstindependenthigh-yieldwellsandpipelinesinthefirstsection,theleadinfactoryproductionandsupplytothepopulation.2.1-3GDPgrowthfigure2.1-4Yibin,Yibincity,Yibincity,fiscalrevenuegrowth2.1.4topographyterrainoverallisSouthwest,North-EasternState.Lowmountainsandhillsinthecitylandscapeasthemainridge-and-Valley,pingbasmallfragmentednaturepictureforwaterandthesecondlandofthesevenhills.236metersto2000metersabovesealevelinthecity,lowmountain,46.6%hills45.3%,pingbaonly8.1%.2.1.5developmentofYibinlandscapesanddistinctivefeatureinthecenterofthecity,withlimitations,andspatialstructureoftypicalzonalgroup,2012-citiesinbuildingwit