PCR实验室SOP文件

PCR2201211PCR2PCR34PCR56PCR78PCR9PCR1011121314PCR15PCR16PCR17PCR18PCR19PCR20PCR21PCR22PCR23PCR24PCR125PTC-20026ABI730027DYY-6C28Bio-RadGelDoc_XR293031323334353637381234567891011121314151617181920PCR12344142RT-PCRPCR4344SOP4546475.1.PCR123441424344SOP45RNAcDNASOP46DNAcDNAPCRSOP47RT-PCRSOP4.8494.10PCR123441a.b.c.d.e.PCRf.g.h.i.4.2a.b.c.d.75%e.60f.g.h.PCR123441a.PCRb.PCRc.PCRd.PCR42a.2b.4315.PCR1.:2.:3.:4.4.1CDC4.2121PCR4.34.44.5,:a)b)c)d)4.6,,,,5.PCR12344142434475%4575%4675%47306048494104115.PCR1234414200100200343a.b.c.d.e.f.5.PCRPCRPCR1234:PCRPCRPCRPCR1PCRPCR2PCRPCR3PCR45PCRPCR6PCR74.1a.b.c.d.e.304.2a.b.c.d.304.3a.b.c.-70d.e.f.g.h.i.j.304.4a.b.c.d.e.RT-PCRPCRf.g.4.5a.b.c.d.e.5.PCRPCR12NaOHEDTA344.14.2()4.34.61510%244.74.85.PCR12PCR344.14.275%4.3f.g.h.15-25i.j.k.l.84m.n.o.305.1.2.PCR344.14.24.3a.b.c.d.15-25e.f.12000rpmg.h.5%i.j.k.l.m.604.4200l1.5ml400lLysisBuffer,10450lBindingBuffer()600l412000rpm1412000rpm1400lWB1412000rpm30600lWB2()412000rpm30412000rpm350lElutionBuffer,2-5412000rpm1-205.1.2.PCR344.1PTC-200ABI7300?4.24.3a.b.c.d.e.15-25f.g.h.i.604.4PTC-200ABI7300a.PTC-200,RUNProceedMAINSelectProceedVesselTypeProceedVolumeProceedUseheatedlidProceedb.ABI73007300SystemSoftwareABI730011Newdocument2233hbv443545104/CtrlHide/ShowWellInspector,5545noneTaskStandard,Quantity50000(6)677SaveDocument,+HBV20060526(8).78(Start),7PCRPCRABI-7300.c.ABI7300A.B.5.PCR12DYY6CBIO-RADGelDocXR344.1?4.24.3a.b.c.d.15-25e.f.g.604.4a.4Start/4/2B.QuantityOneFileGelDocXR,Live/Focus11UV,White12IRISZOOMFOCUS13AutoExpose,0.15%OptionsManualExpose14Freeze15Analyze165Save5.5.15.26.123441424h-7042a4-70b-70c43-705.PCRPCR123441411412a.b.2-3cmc.5gd.35mle.1.02.0ml422RNA4mol/LGITCDNAEDTA45451452a.()b.123mL5ml345RNA26c.abc5.PCRPCRPCR123441422%2%4375%4430604546844748492%4104114125.PCR124%NaOH75%2%3444441250ml1000ml1000ml100ml2000ml44244214%NaOH:a4NaOHb250ml100mlNaOHcNaOHd100ml4422:a500mg/Lb20/500g1000mlc442375%:a75%bRNA75%DEPC44242%:a20ml1000mlb980ml1000ml2000ml14PCR12344.14.1.1001ABCD------------ABCD/001002-----------------------------ABCD/002003PCR----------------------------ABCD/003004PCR------------------------------ABCD/004005-------------------------------------ABCD/005006-------------------------------ABCD/006007PCR----------------------------------ABCD/007008-------------------------------------ABCD/008009PCR----------------------------ABCD/009010---------------------------------ABCD/010011-------------------------------------ABCD/011012PCR----------------------------------ABCD/012013-----------------------------------------ABCD/013014PCR-------------------------ABCD/014015-------------------------------------ABCD/015016-------------------------------------ABCD/016017---------------------------ABCD/017018-------------------------------ABCD/018019-------------------------------------ABCD/019020PCR-----------------------------ABCD/020021-------------------------------------ABCD/021022-------------------------------ABCD/022023PCR-------------------------------------ABCD/023024-------------------------------------ABCD/024025-------------------------------ABCD/0254.1.24.1.3,,20035200305SYJG200305SJXH200305SLZK254.1.44.1.54.24.2.1D4.2.2200312Jan01Feb024.2.3abc20035820030508a20030508b4.2.4USBPCCD2003,PCR123441424344d.e.f.g.h.i.j.4546475.12344142434445451104105106108452aCT25b453454455CT54562.93.926340.99465.PCRPCR121.5ml0.5ml344.14.24.34.455.10.5ml1.5ml5.1.1:5.1.215022035010000rpm204655.25.2.15.2.215021036.PCRPCR123441411HBVDNA106copies/ml0.5ml110lQb0201-n-2041210413442421aXX2sX3sbcXsCV422aX3sbX2s6423abc661.56d424aXsXsXsbCTXsXXscCV5.PCRPCR1EQA2CDCCDCPCR344.1-204.24.34.4EQA14.5EQACDCE-mail4.64.75.12344.14.1.114.1.24.24.2.14.2.24.2.34.2.44.2.54.2.64.34204.3.14.3.24.3.34.44.4.14.4.24.4.34.4.44.4.55.PCRPTC-200PCR1PE57002PE570034.:5.5.1DNA5.2EnterProceed5.3SelectProceedProceedProceed5.4Proceed5.5TempProceed-5.0105.0ProceedProceedYesProceedGoToProceedProceedProceedYesProceed5.6EndProceedYesProceedProceed5.7RunProceedProceedSelectProceed5.8Block5.9Calculated-controlTUBESProceedThinThickProceed5.10Proceed5.11YesNoProceed6.PCR6.175%6.275%ABI73001ABI73002ABI73003?4.:5.7.DYY-6C12DYY-6C344.14.24.34.44Start4.54.64.7199594.8/4/2UsIsTsUIT4.9124.10UsIsTsTDYY6CUs=400VIs=400mATs=130T=100---StartT=000---Goon4.11UsIsTs8UIT4.12/stopGoonStart0004.13stop4.141stop2No_Load3Over_Load4Over_U4Over_I65.5.15.25.35.45.55.65.75.8100END6.1231234.:5a.b.c.d.e.f.0.16.(1),,,(2),(3)5.(4)6.(1)(2)712344.14.24.34.475%1234.:551525354555670%/576.(1),,,,(2),,.(3),,.(4)1.2g/cm3,n:n=nmax*--1.2/.(5)6mm,.(6).(7).(8),,,.(9)5.6,5.7(10)7.8.1234.:58.1234.:5515253545570%/566.(1),,,,(2),,.(3),,.(4)1.2g/cm3,n:n=nmax*--1.2/.(5)6mm,.(6).(7).(8),,,.(9)5.6,5.7(10)7.(1)(2)ICSH8.1234414-202424344454647485.6.1.2.SOPXXXXXX344.1ON4.2-70SET-85887-75550SET-704.310104.3ALARM4.414.54.65.12SOP34.:5515.270%5.3153051015.45.55.65.75.85.95.101015305.112%841%845.126.6.1706.2MEK--6.36.46.5366.610.3m/s6.77.8.12SOP34414243444530min30min461%8430min7.8.123441411412Tip413ab2~3mmc1d414/ab2~3mmcd415ab2~3mmcdecd4275%175%15434312025