氨氮厌氧氧化的微生物反应机理

收稿日期:2000-04-28*基金项目:;(980598);作者简介:(1965-),,,.氨氮厌氧氧化的微生物反应机理*周少奇(,510640)摘要:NH+4,NO-2NO-3,,,CHON=C5H7O2N,.关键词:氨氮;厌氧生物氧化;计量学;电子流平衡中图分类号:X173文献标识码:A文章编号:1000-565X(2000)11-0016-04,,,,,,.,..,(1):NH+4NO-2NO-3NO-2N2(1)|||,().,,COD,,.,,,,COD()NO-2NO-3N2[1],NO-2NO-3,.1氨氮厌氧生物氧化现象的发现与优点1995,MulderVandeGraaf[2,3],(AnaerobicAmmoniumOxidation_Anammox,AOA_AnaerobicOxidationofAmmonium),Broda[4]1977(denitrifyingammonia_oxidizers).,NH+4,NO-3NO-2,N2,[4,5]:NH+4+NO-2N2+2H2O(2)G0=-361kJ/mol(自然科学版)2811JournalofSouthChinaUniversityofTechnologyVol.28No.11200011(NaturalScienceEdition)November2000NH+4+0.6NO-30.8N2+1.8H2O+0.4H+(3)G0=-297kJ/mol(1997),:-,[5].(1),,,,;(2)1molNH+42mol,,1molNH+40.75molO2,62.5%,.(3)1molNH+42molH+,1molNO-3NO-21molOH-,,,.2氨氮厌氧生物氧化反应的机理2.1氨氮厌氧生物氧化反应的计量方程式[6,7],(NH+4)(CHON)fs,1mol,:NH+4,NO-3,NO-3.18NH+4+(1-fs5-18+fsP)NO-3+fsPCO2=fsPCHON+1-fs10N2+1+4fs20-fsPH++9-24fs40+(2+3-)fsPH2O(4)NH+4,NO-3,NH+4.S+8fs8SNH+4+3-8fs40NO-3+(-)fsSCO2+fsSHCO-3=fsSCHON+1-fs10N2+1+4fs20H++940-3fs5+(2+-)fsSH2O(5)NH+4,NO-3(NO-2N2),NO-218NH+4+(3-4fs8+fsP)NO-3+fsPCO2=fsPCHON+1-fs2NO-2+14-fsPH++1-4fs8+(2+3-)fsPH2O(6)NH+4,NO-2,NO-2.16NH+4+(1-2fs6+fsP)NO-2+fsP)CO2=fsPCHON+1-fs6N2+(P-3)fs3PH++1-2fs3+(2+2-)fsPH2O(7)NH+4,NO-2,NH+4.S+6fs6SNH+4+1-2fs6NO-2+(-)fsSCO2+fsSHCO-3=fsSCHON+1-fs6N2+fs3H++1-2fs3+(2+-)fsSH2O(8)11:17:P=4++5-2P=4++3-2S=4+-3-2(9)P,P,S.2.2计量方程式的简化,C5H7O2N.CHON=C5H7O2N,(9)P=28,P=26,S=20,(4)~(8),:18NH+4+7-46fs280NO-3+5fs28CO2=fs28C5H7O2N+1-fs10N2+7+23fs140H++63-58fs280H2O(10)5+2fs40NH+4+3-8fs40NO-3+fs5CO2+fs20HCO-3=fs20C5H7O2N+1-fs10N2+1+4fs20H++9-6fs40H2O(11)18NH+4+7-26fs56NO-3+5fs28CO2=fs28C5H7O2N+1-fs2NO-2+7-fs28H++7-6fs56H2O(12)16NH+4+13-23fs78NO-2+5fs26CO2=fs26C5H7O2N+1-fs6N2+23fs78H++13-11fs39H2O(13)10+3fs60NH+4+1-2fs6NO-2+fs5CO2+fs20HCO-3=fs20C5H7O2N+1-fs6N2+fs3H++20-13fs60H2O(14),fs=0,(),(10)~(14):18NH+4+340NO-3=110N2+120H+940H2O(15)18NH+4+340NO-3=110N2+120H++940H2O(16)18NH+4+18NO-3=12NO-2+14H++18H2O(17)16NH+4+16NO-2=16N2+13H2O(18)16NH+4+16NO-2=16N2+13H2O(19):(15)=(16)=(3),(18)=(19)=(2).,NO-xNH+4,.,,:1828(1)(AOA)CO2,AOA.(2)AOA,NO-x,NH+4.(3)AOANH+4,.(4)AOA,H+,pH.4结束语(AOA);,,;NH+4,AOA;(AOA)pH.AOA,,.AOAAOA.参考文献:[1],.COD/N_NH+4[J],,2000,22(1):18-21.[2]MULDERA.VandeGRAAFAA,ROBERTONLA,etal.Anaerobicammoniumoxidationdiscov-eredinadenitrifyingfluidizedbedreactor[J].FEMSMicrobiolEcology,1995,16:177-184.[3]VandeGRAAFAA,MULDERA,BRUIJNP,etal.Anaerobicoxidationofammoniumisabiolog-icallymediatedprocess[J].ApplEnvironMicrobiol,1995,64(4):1246-1251.[4]BRODAE.Twokindsoflithotrophsmissinginnature[J].ZAllgMicrobiol,1977,17:491-493.[5],.[J].,1997,19(4):25-28.[6].[J].,1998,26(3):124-126.[7].[J].,1999(3):30-32.MicrobialBiochemicalReactionsofAnaerobicOxidationofAmmoniumZHOUShao_qi(Dept.ofEnviron.Sci.&Eng.,SouthChinaUniv.ofTech,Guangzhou510640,China)Abstract:BasedonMcCartyshalfreactionconceptandZhousmethod,thestoichiomet-ricequationsofanaerobicoxidationofammonium(AOA)areyielded.ItisdiscoveredthatAOAprocessisakindofautotrophicbioprocess.TherearetwopossibilitiesofthenitrogensourceforcellsynthesisofAOA,i.e.,NO-xorNH+4.ThealkalinityisnecessaryforAOAprocesswhenNH+4isusedasthenitrogensourceforcellsynthesis.ItispredictedthatpHvalueswilldecreaseifAOAprocessesareachieved.Keywords:Ammonium;anaerobicbiologicaloxidation;stoichiometry;electronflowbalance11:19