近海海泥链霉菌的分离方法

:2007208229:(06025388):(1973-),,,,:02087597453,E2mail:[e-mail]1,,,(,510640):NaCl:4020min,,1HV,,60%80%NaCl2%3%,:;;:S154.383:A:100529261(2007)20053204StudyonIsolationofStreptomycesfromSeaSoilofOpen2seaSHENHui2fang,LINBi2run,XIEShuang2da,ZHANGXiao2yong(InstituteofPlantProtection,GuangdongAcademyofAgriculturalSciences,Guangzhou510640,China)Abstract:TheinfluencesontheisolationeffectsofStreptomycesfromseasoilofopen2seaandsaltfieldwerestudiedbyplatepaintisolationmethod,whichweredrytreatmentofsoil,thermaltreatmentofsoilsuspension,mediavariety,concentrationofseawaterandNaCl.Theresultsshoweddrytreatmentofsoilcouldobviouslydecreasethenumberofbacteria,thermaltreatment(40,20min)insoilsuspensionmightpromotethesproutofStreptomycessporeandincreasethequantityofStreptomyces.GSandmodi2fiedHVmediawerethebesteffectontheisolationofStreptomyces,therewaslittleinferenceofbacteriaanditwaseasytoselecttheStreptomycescolony.ItwasapplicabletoisolateStreptomycescolonywhentheconcentrationofseawaterwasfrom60%to80%ortheconcentrationofNaClwasfrom2%to3%inthemedia.Keywords:seasoilofopen2sea,Streptomyces;isolationtechnique,,3523()53-56ChineseJournalofBiologicalControl20079,[1],(Streptomyces),2/3,,,:,,[2],,,11.1,No20()No41()1.2:1,HV(g/L):200mlNa2HPO40.5gKCl1.7g15g1.31g,10ml,,20min,10-210-310-410-5,0.2ml,,428710d,,22.1,4,28,,,(1)1(10-3)No20No4147.254.57a89.0010.10a66.755.06a141.514.15a40.002.58b28.503.32b59.256.29a37.007.16b:,5%(DMRT),2.2,,,[3],20min,2:4020min;50,,60,2.3,4020min,4523,3:,HV,4HVHV,,,HV,,,2(10-3)()No20No412837.007.68ab24.505.00a61.754.86ab33.254.42ab4046.508.35a25.254.99a69.756.14a36.752.50a5033.004.08b26.753.50a45.506.46b30.006.32ab6014.252.99c12.502.52b18.004.24c15.502.52c3(10-3)No20No4145.504.57a8.001.63a22.004.24cd63.504.12a21.501.91a37.255.12d15.002.45c3.501.00bc62.258.06b21.504.80d8.251.71d75.005.97bc27.004.40b6.501.00a30.756.02c35.254.43c13.000.82c56.506.19c18.504.03c4.750.96cb68.58.58ab26.753.30cd6.001.41e84.009.06b6.002.94d2.000.82c79.509.71a11.002.71e3.250.50f118.5011.47aHV39.005.48ab7.750.96a12.002.58d55.005.66b18.251.89b22.506.19e2.4,020%40%60%80%100%,,4020min,460%80%,100%No4160%100%20%40%,,,4(10-3)(%)No20No4110040.254.03b29.502.89b66.757.27cb38.504.20b8046.003.65a37.752.75a77.007.83a42.754.43b6043.753.30ab33.002.94b80.256.98a56.002.16a4029.004.35c19.503.79c61.003.83b40.255.32b2016.252.99d11.503.00d31.504.65c20.003.16c09.251.50e7.751.26d14.501.91d11.752.22c2.5NaCl,NaCl,NaCl1.0%1.5%2.0%2.5%3.0%,3.5%4.0%,,55:,4020min,5NaCl2%3%NaCl3.5%,,NaCl5NaCl(10-3)NaCl(%)No20No411.012.752.50de4.001.830ef19.502.52e9.502.65d1.521.002.58c9.752.990d31.002.94d16.252.50c2.032.753.77b15.251.890bc45.504.19b23.503.00b2.535.503.70ab18.752.500b48.754.19b26.752.63b3.031.505.20b13.502.080c38.503.00c24.502.38b3.517.253.30cd7.251.260de22.502.52e16.251.71c4.010.001.63e2.501.000f11.762.06f10.250.50d40.753.30a25.254.570a55.754.50a32.253.30a3,1HV,,,,,,,,,[4]4020min,,,[3],,1HV,,,[5],60%80%,,,2%3%NaCl,,[1],.[J].,2003,22(2):42-44.[2],,,.[J].(),2001,22(1):1-4.[3],,.[J].,2004,31(2):61-65.[4]CrossTA.Actinomycetessourceofnewmetabolites[J].DevIndustMicrobiol,1982,23:1-18.[5],,,.[J].,1999,(3):15-18.6523