桃果实中花青素的研究进展-赵秀林

*430023、、。、、、pHHPLC、γ、、、、、、、。S662．1A0517－6611201210－05735－02ResearchProgressofAnthocyanininPeachFruitZHAOXiu-linetalSchoolofBiologyandPharmaceuticalEngineeringWuhanPolytechnicUniversityWuhanHubei430023AbstractTheresearchprogressofanthocyanininpeachfruitathomeandabroadinrecentyearshavebeensummarizedwhichincludetheextractionmethodsdetectionmethodspharmacologicalactivitiesresearchandthefactorsthataffectthesynthesisofanthocyanininpeachfruitetc．．Inadditionitpointsoutthatthemainextractionmethodsofanthocyanininpeachfruitaresolventextractionmethodpressurizedsolventextractionmethodultra-highpressureassistedextractionmethodmicrowave-assistedextractionmethodandultrasonic-assistedextrac-tionmethodetc．hereintothesolventextractionmethodisthemostcommonthedetectionmethodsmainlycontainpHdifferentialmethodandHPLCthefactorsthataffectthesynthesisofanthocyaninincludebaggingγ-rayilluminationexogenoushormonessolublesugarti-tratableacidenzymeandgeneetc．moreoveranthocyaninhasthefunctionsofantioxidationantimutageniceffectanticancereffectandcardiovascularsystemprotection．KeywordsPeachAnthocyaninExtractionInfluencingfactorsPharmacologicalactivities2010cx0003。1986－。*[e-mail]。2011-12-261。、、、2。、、、、。、、3。、、。1、、。、、、。。4℃24h3112h26h36h0．05%4。Vizzotto95%∶1．5mol/LHClV/V85∶155。2、HPLC、HPLC/MS、。2。2．1pHpHpHpH。PeerzadapH1．0、4．56。2．2HPLCHPLCApH3．0B0～5minB0～25%5～15minB25%～65%15～17minB65%～100%2min17～19min19～22minB100%03min。11．02min-3-132．26±0．80μg/gFW4。WuHPLC-DAD-ESIMSA5%B252-3--3-450～2660μg/gFW7。3、、、8。3．1Jia528nmOD0．41OD1．83JournalofAnhuiAgri．Sci．201240105735－57365750。9。Kim1．96μg/cm21．01μg/cm2。10。3．2γPhenylalanineammonial-yasePALFlavonoidglycosyltrans-feraseGT。Peerzadaγ。1．6～2．0kGyPAL。γγP＜0．051．6kGy。PALGT26。3．3。。Shiraz11、、、12。13。14。3．4GenisteinGNT22d40～120μmol/LGNT15。DehydroepiandrosteroneDHEA870%～80%DHEA-6-Glucose-6-phosphatedehydrogenaseG6PDH8d20%。DHEAG6PDHG6PDH16。3．5、17。、18。3．6PAL、-4-Cinnamate-4-hydroxylaseC4H、4-CoA4-coumaratecoen-zymeAligase4CL。ChalconesythaseCHS、ChalconeisomeraseCHI、-3-Flavanone3-hydroxylaseF3H、Dihydroflavonol-4-reductaseDFR、AnthocyaidinsynthaseANS-3-O-Flavonoid3-O-glucosyltransferaseUFGT。OxidationpentosephosphatepathwayOPPPG6PDH1。。UFGT、PAL0．0519。G6PDHPALG6PDHG6PDHPALG6PDH16。3．76CHS、CHI、F3H、DFR、ANSUFGTCHS、CHI、F3H、DFR、ANSUFGT。Tsuda、6CHSDFRCHSDFR62。CHSDFR20。44．1Vizzotto11-Diphenyl-2-picrylhydrazylDPPH、221。C7。McGlynnOxygenRadicalAbsorbanceCapacityORACORAC3．90～5．02mol/LTE/gORACORACR=0．84522。ByrneORAC440～1780μgTE/g575063752012。324-D24-D0．5mg/L。3。2。。。7、、、、。1．J．2003324－25．2．J．2006576－82．3．J．2006669－70．4．J．201051232－1236．5．J．200735319890－9892．6．J．2009404495－497．7．J．20043檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪117－119．573623。4．2ByrneMDA-MB-435MCF-7MCF-10AMBA-MD-435LDL24。LeaCaco-2、SW1116、HT29NCM4604Caco-2C18LH-2025。5、。1．J．2006233359－364．2．AFLPJ．2008472138－141．3．J．200117360－62．4．、J．2004212167－169．5VIZZOTTOMCISNEROSLOKIEWRetal．AntioxidantactivityandthecontentofphenolicsandanthocyaninsinpeachandplumJ．JournaloftheAmericanSocietyforHorticulturalScience2007132334－340．6PEERZADARHALIMWRAGHUVEERSMetal．GammairradiationinducedenhancementofphenylalanineammonialyasePALandantioxi-dantactivityinpeachJ．RadiationPhysicsandChemistry201079982－989．7WUXLPRIORRL．Systematicidentificationandcharacterizationofan-thocyaninsbyHPLC-ESI-MS/MSincommonfoodsintheunitedstatesFruitsandBerriesJ．JournalofAgriculturalandFoodChemistry2005532589－2599．8．J．20112310938－944．9JIAHJARAKIAOKAMOTOG．Influenceoffruitbaggingonaromavol-atilesandskincolorationof‘Hakuho’peachPrunuspersicaBatschJ．PostharvestBiologyandTechnology20053561－68．10KIMYHKIMHHYPUNCKetal．Effectsofbaggingmaterialonfruitcolorationandqualityof‘JanghomonHwangdo’peachJ．ActaHorticul-turaeISHS200877281－86．11DOWNEYMDHARVEYJSROBINSONSP．Theeffectofbunchshad-ingonberrydevelopmentandflavonoidaccumulationinShirazgrapesJ．AustralianJournalofGrapeandWineResearch20041055－57．12TAKOSAMJAFFEFWJACOBSRetal．Light-inducedexpressionofaMYBgeneregulatesanthocyaninbiosynthesisinredapplesJ．PlantPhysiology20061421216－1232．13．J．20102613308－311．14．“”“”J．200723272－76．15．J．2007343569－574．16．－6－J．20112713232－237．17．J．2010453307－317．18．J．2008253312－315．19．J．200735164755－47564759．20TSUDETYAMAGUCHIMHONDACetal．ExpressionofanthocyaninbiosythesisgenesintheskinofpeachandnectarinefruitJ．JournaloftheAmericanSocietyforHorticulturalScience20041296857－862．21VIZZOTTOMCISNEROS-ZEVALLOSLBYRNEDH．LargevariationfoundinthephytochemicalandantioxidantactivityofpeachandplumgermplasmJ．JournaloftheAmericanSocietyforHorticulturalScience20071323334－340．22MCGLYNNWMCCRAWBD．CorrelationofoxygenradicalabsorbancecapacityORACvaluewithanthocyaninandphenoliccontentofpea-chesJ/OL．http//．phtnet．org/research/download/pdf/md387．pdf．23BYRNEDHCISNEROS-ZEVALLOSL．Anthocyaninandphenoliccon-tentinredfleshedpeachesandplumsandtherelationshiptoantioxidantactiviyC//FifthInternationalPeachSymposium．DavisCAActaHort2001．24BYRNEDHNORATTOGCISNEROS-ZEVALLOSLetal．Healthben-efitsofpeachnectarineandplumsJ．ActaHort2009841267－274．25LEAMAIBEHCDESBORDESCetal．Inhibitionofgrowthandinduc-t