木质素含量的检测方法-李海涛

21220114LEATHERSCIENCEANDENGINEERINGVol.21No.2Apr．20111004-7964201102-0031-042010-08-28200803033-A004C1985-。*[e-mail]。*610065。。O636．2ATheDetectingTechniquesofContentofLigninLIHai-taoYAOKai*JIADong-yingHEQiangCollegeofLightIndustryandFoodSichuanUniversityChengdu610065ChinaAbstractbythecomplexchemicalstructureandspecificphysicalandchemicalpropertiesofligninthedeterminationofitsbecomesverydifficult．Thedetectionmethodsofligninarereviewedandtheprinciplesandfeaturesofthemethodsaredis-cussedinthispaper．Moreoverthedevelopmenttrendinthedeterminationmethodsofligninispreviewed．Keywordslignindetectionmethodsprinciples115%～30%。、、、1。β－O－4、β－5、β－β、5－5、5－O－4、α－O－4、、123。、、。。22．1KlasonKlason。－1∶2v/v72%25℃2h3．0%4h105℃28．93%RSD0．15%4。Klason。2．232211Fig．1Chemicalstructureandbondingmodesoflignin200～760nm。200～300nm5。－70℃30min。2mol/LNaOH260nm2．47%RSD2．4%Klason1．69%RSD3．4%6。、、。2．3。、、、、。14ODSC18－－1∶1∶8v/v1．0mL/min27℃280nm。2．0%1．0～10μg/L7。2．4。。、、。、、72%660nm8。Kla-son。2．5。50%50%72%16h10%10%0．025mol/L20%1．0%。10%0．025233mol/L。27．43%9。2．6。N－N－50%50%N－N－N－N－N－。DB－530m110℃240℃。1、/、10。1Tab．1ComparisonofphenolichydroxylcontentinligninsdeterminedbyaminolysisandUV-spectroscopy/%/%/%/%4．600．044．500．32/4．900．074．400．302．550．082．300．362．7。20%80%1%。211。2Tab．2Comparisonofcarboxyliccontentinligninsdeterminedbydifferentmethods/%/%/%/%/%/%5．970．507．100．317．060．15/5．420．566．900．376．910．221．820．692．170．412．020．272．8—CH—OH—NH。。0．990．970．36%0．59%12。－。0．01～1．0μg/mL125600。－342113。3、Klason14。15－16。、。1NourEddineElMansouriJoanSalvado．Analyticalmethodsfordeterminingfunctionalgroupsinvarioustech-nicalligninsJ．IndustrialCropsandProducts200726116－124．2HiguchitT．Ligninbiochemistrybiosynthesisandbio-degradationJ．Woodscienceandtechnology19902423－63．3MayraALaraaAntonioJRodriguez－MalaverOrlandoJRojas．BlackliquorligninbiodegradationbyTrameteselegansJ．InternationalBiodeteriorationandBiodegra-dation200352167－173．4．J．200223272－73．5HaiqiangShiBingyueLiuXingqiangLietal．Thelig-nincontentinblackliquidofstrawAS－AQbyultravioletspectrophotometerJ．JournalofTianjinuniversityofscienceandtechnology20041991－96．6．J．2006293239－241．7LoboIMozetoAACassQB．Determinationofphenol-iccompoundsfromoxidationofligninlakesedimentsbyhigh－performanceliquidchromatographyJ．Chroma-tographia20005211－12727－731．8．J．20046368－369．9．J．200810564－65．10WegenerGStrobelC．Determinationofphenolichydrox-ylgroupsinligninsandligninfractionsbymeansofamin-olysisandFTIRspectroscopyJ．HolzalsRoh－undwerkstoff199250417－420．11GosselinkRJASemkeHMalherbeRetal．Analyti-calprotocolsofcharacterizationofsulphur－freeligninJ．IndustrialCropsandProducts200419271－281．12．KlasonJ．200727101977－1980．13KapatADeyS．AnalternativeapproachtothedetectionofligninanoteontheapplicationofELISAusingpoly-clonalantibodiesJ．BioprocessEngineering20002275－77．14AlvesASchwanningerMPereiraHetal．CalibrationofNIRtoassesslignincompositionH/Gratioinmari-timepinewoodusinganalyticalpyrolysisasthereferencemethodJ．Holzforschung200660129－31．15XiaoyanWenHongpingHeJianxiZhuetal．Arrange-mentconformationandmobilityofsurfactantmoleculesintercalatedinmontmorillonitepreparedatdifferentpilla-ringreagentconcentrationsasstudiedbysolid－stateNMRspectroscopyJ．JournalofColloidandInterfaceScience20062992754－760．16BalakshinMYCapanemaEAGoldfarbBetal．NMRstudiesonFraserfirAbiesfraseriPurshPoirLigninsJ．Holzforschung2005595488－496．