金银花中总黄酮提取条件优化研究

,(,453003)[][],,[]:3.5h,60,127,70%[],;;;S567.7+9A0517-6611(2008)32-14151-02StudyontheOptimumConditionofExtractionofTotalFlavoneinLonicerajaponicaSONGLin2linetal(HenanInstituteofScienceandTechnology,Xinxiang,Henan453003)Abstract[Objective]TheaimwastostudyontheoptimumextractionprocessforthetotalflavonefromLonicerajaponica.[Method]Colori2metricmethodwasusedtodeterminethetotalflavoneofLonicerajaponica.Orthogonalexperimentwasusedforthestudyontheeffectsofetha2nolconcentration,volume,extractiontimeandtemperatureonextractionprocessoftotalflavonefromLonicerajaponica.[Result]Theopti2mumextractionprocessoftotalflavonefromLonicerajaponicawas127solid2liquidratio,70%ethanoland3.5hextractiontimeunder60.[Conclusion]Thisextractionprocesswassimple,stableandefficient,whichofferedbasisfortheeffectiveuseofLonicerajaponica.KeywordsLonicerajaponica;Totalflavone;Orthogonaltest(0625)(1981-),,,,,2008209204(Lonicerajaponica),[1][2],[3],[4],,,,11.11.1.1:95%;1.1.2BS2110S(Sartorius);RE252C();();DK29821();8122();();7221.21.2.11.2.220mg,95%100ml,95%,00.51.02.03.04.0ml610ml,5%NaNO20.3ml,6min10%Al(NO3)30.3ml,,6min;4%NaOH4ml,95%,12min,1cm505nm,A,C:A=12.2100C-0.0061,r=0.99991.2.35g,,,,,50ml,1.2.41ml25ml,95%12.5ml,1.2.2,1.2.54(1)5g,120,70%70,1h,(2)5g,120,70%5060708090,3h,(3)(70,70%,3h),,(4)(70,120,3h),,,43,JournalofAnhuiAgri.Sci.2008,36(32):14151-1415222.12.1.11,,,4h,4h,,,4h1Fig.1Effectsofextractiontimeonextractionrate2.1.22,,,60,,,602Fig.2Effectsofextractiontemperatureonextractionrate2.1.33,125,,1253Fig.3Effectsofethanolvolumeonextractionrate2.1.44,70%,,70%2.21,,:4Fig.4Effectsofethanolconcentrationonextractionrate:A1B2C3D2,3.5h,60,127,70%,8.7%1L9(34)Table1ResultofL9(34)orthogonaltestNo.hATimeBTemperatureCSolid2liquidratioD%Ethanolconcentration%Extractionrate13.555122655.523.560125706.233.565127756.844.055125754.254.060127654.864.065122705.174.555127705.984.560122755.294.565125653.9R1.4670.2001.0661.0003,,[5],,[6],,,,:3.5h,60,127,70%[1].[J].:,2006,26(2):131-134[2],,,.[J].,2002,18(4):66-68.[3],,.[J].,2005,27(2):239-241.[4].[J].,2004,10(2):144-145.[5]..()[M].:,1996.[6],,,.[J].,2002,23(2):62-66.251412008