植物多糖的提取方法和工艺

,200683NO.3JOURNALOFFUJIANFISHERIESAug.25.2006(,361005):,,,:;(),,,,,,[1],100,,,,,,,,(),,,1[2],,,,,,,,,,,,,;,;,;;,[3][4]5,:110,115,13,19;:80~95;13:~3h5:518%532%282%188%183%[5],:20~100,(20~50),30~240min205%[6]:(102),pH30,3h,140[7],,50%,3,15h;41,Sevag12,15min75%,2,1h:31,Sevag12,45min50%,1,05h;41,Sevag21,45min,,,,,,,2,[8],:100g,1000ml01molPLHCl,1h,,,1/5,95%30%,,70%,,,C2335%[9],,,:1000g,5%HC1,,EtOH70%,,2500rpm,,95%EtOH3,4%HCl,1%,,4,,,3,,10g(A),,,,3,,[3]:01~1M,,HayashiKatsuhiko[10],:,,,,pH10,pH3~4,,,,[11],,433110,,,,,,,,[2][12],2(,),1(+)(pH=7)(pH=2)2,,,:3%,40pH=7,6515h,25%,pH=7,50lh,40.80,15h[6]:8%(/),4%(/),pH30,3h,50,,5,,,,(,),,,[13]1(PVDF),(lipopolysaccharide,LPS),855%896%[14],,,,,[14],:,,,,6[2],20KHz,,,,,(,),,,,,,,,,,,,,[15],,,,,,[12],343:60min,40,60min,70%:[16],[17][18]7300MHz300GHz,,,,,,,,,,[19],,[20][21][22][21],:,,745%,205%,200W8min401[23],,,20%,,60min,151,,,,,,[24],,,,,[1]FranzG.Polysaccharidesinpharmacology.Currentapplicationandfutureconcepts[J].PlantMedica,1998,55:493.[2].:[].:,2004.[3],..,2005,32(3):179-184.[4],,.[J].,2002,18(2):96-100.[5],.[J].,2005,4(3):265-267.[6].:[].:,2003.[7].RAPD:[].:,2004.[8],,.[J].,2004,25(5):429-430.[9],.[J].,2005,24(1):35-36.[10]HayashiKatsuhiko.Preparationmethodofacidicpolysac2charide[P].JP:2001-288202,2001-10-16.[11]AkiraY,HiroshiNM,TakaSB.,etal.PlantaMed,1986,3:213~218.[12].:[].:,2003.[13].[J].,2000,26(6),333-335.[14],.[J].,2005,16(2):228-35110230.[15],,,.[J].,2003,9:33.[16],.[J].,2001,(1):58-61.[17],.[J].,2002,16(1):7-8.[18],.[J].,1999,72(4):19.[19],.[J].,2005,17(1):42-44.[20],,.[J].,2005,16(10):965-966.[21],,,,.[J].,2005,21(2):153-156.[22],,.[J].,2005,16(7):594-595.[23],.[J].,2003,10:17-19.[24],.[J].,2004,24(3):113-118.ExtractionMethodsandTechniquesofPolysaccharidesXUYanyan(DepartmentofChemicalandBiochemicalEngineering,XiamenUniversity,Xiamen361005,China)Abstract:Thepolysaccharidehasmanykindsofbioactivitieswhicharewatchedbyalotofresearchers,anditsextractionmethodsandtechniqueshavebecomeoneofthefocusesofthepresentresearches.Inthestudyofpolysaccharidesextractionforplants,alargeamountofmethodshavebeenused,suchassolventextraction,acidextraction,alkaliextraction,enzymeextraction,ultrafiltrationextraction,ultrasonicextraction,andmicrowaveassistedextraction(MAE).Thispapersummerizestheextractionmethodsandtechniquesofthepolysacchridesmentionedabove,inthehopeofprovidingtheresearchworkinthisfieldwithsomereferrences.Keywords:plantpolysacchride;extraction36