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http//.biother.orgChinJCancerBiotherJun2015Vol.22No.3doi10.3872/j.issn.1007-385X.2015.03.021··DNAResearchprogressofcirculatingtumorcellsandcirculatingtumorDNAincolorectalcancer200433No.8147277081272280。ProjectsupportedbytheNationalNaturalScienceFoundationofChinaNo.81472770812722801984-E-mail759051596@qq.comYanHonglicorrespondingauthorE-mailhongliyan@smmu.edu.cn、、。DNA、、。DNA、。DNAR735.2R730.4R730.54A1007-385X201503-0393-06colorectalcancerCRC1。11%20%~45%2。、。。CRCCRCcirculatingtumorcellsCTCsDNAcirculatingtumorDNActDNA。CTCs3。ctDNADNADNADNADNADNADNADNA、CTCs4。CTCs、、、“”5。ctDNA、、6。CTCsctDNA。1CTCsctDNACTCsDNA、RNAmRNAormicroRNACK8、CK18、CK19、CK20、EPCAM、hTERT、CEA、CA199。、CDmRNA。circulatingtumorstemcellsCTSCsepithelialmesenchymaltransitionEMTCTCs7。1.1CTCs1.1.1CTCsCTCs10mlCTCs“”CTCs。1892CTCs103、、·393·20156223CTCs。Chen11CTC-ChipEPCAMCTC-ChipCTCs。Sun12CTCs。1.1.2CTCs1CTCs、DNA、CTCs———cytokeratinCK8。Fusi13FCMCTCsCTCs。CTCs1。2CTCsFDACellSearchepithe-lialcelladhesionmoleculeEPCAMCD45CK+、DAPI+CD45-CTCs。、EPCAMCTCs14。EPI-SPOTCTCsCTCs15。Xu16CTCs、CTCs。Hughes17CTCsE-CTCsCellSearch。Heitzer18CTCs。1CTCs+CTCsCTCsFISH+++CTCs1.2ctDNADNAcellcirculatingtumorDNActDNADNA。DNA1、DNA2DNA19。ctDNA、、、20。ctDNActDNA、21。、SYBRGreen1、PicoGreen、Hoechst、、PCR、DNAbDNA22。·493·.DNActDNA、DNA/BRAF、APC、erB-2、EGFRMyc。21。ctDNA。23。PCR。DNAAPC、DAPK、P1GSTP1、MGMT、P16、Ras1ARASSF1AG2RARe2ctDNA21。DNACpGPCRMSPDNA。ctDNActDNA。PCRctDNA。Newman24ctDNAcancerpersonalizedprofilingbydeepsequencingCAPP-Seq。PCRPCRctDNA25-26。2CTCsctDNACRC80%。DukeA595%DukeB575%DukeCD530%~40%CRC。Sastre2760%DukeBCTCs85%DukeCDCRC1~5CTCs/7.5mlCTCsCRC。Bettegowda28PCR640ctDNACTCs75%ctDNACTCs50%。CTCsctDNActDNACTCs。PCRCTCsDNACRC29。CTCsCTCs、CTCsDNADNActD-NACRC50CTCsctDNACRC30。3CTCsctDNACRCCRCCTCsctDNA。CTCs。CRCEGFRK-RasEGFR31。CRCCTCsK-Ras32-33。CTCsK-Ras。Yakabe34CA199CTCsIVCRC。Tol35372CRCCTCsCTCs29%211%CTCs。Matsusaka3664CTCs<3progression-freesurvivalPFSoverallsurvivalOSCTCs>3。Iinuma37735CEA、CK、CD133mRNAPFS。CEA、CK、CD133mRNADukesC。CTCsCTCs·593·20156223。ctDNA。ctDNA。、ctDNA。Pu38CRCPCRPNAPCRctDNAK-Ras。Misale39K-RasmCRCEGFRctDNAK-Ras。PIK3CAK-RasPIK3CAK-Ras。40-41。ctDNA“”。ctDNACTCs42。4CTCsctDNACRCCTCsctDNAPET-CT。CTCs。Aggarwal43CTCsCEAOSCTCsCTCsCEAmCRC。Wong44CK20CDX2CTCsOS。Yokobori45Plastin3CTCsmCRC。Albuquerque46RTqPCRCTCsKRT19、MUC1、EPCAM、EACAM5BIRC5DCRC。Barbazan47qRT-PCRCTCsCRC。Rahbari4836MetaCRCPFSOSCTCs。Dolores49CRCctD-NA。ctDNA。Diehl50CRCAPCP53K-Ras100%ctD-NA。Frattini51ctDNA、。CRCctDNActDNAK-RasP16。K-RasP16ctDNA。McBride52ctDNA。ctDNA。5CTCsctDNACRCCTCsctDNACRCCTCsctDNA。ctDNACTCs。CTCsctDNA531ctDNA、234CTCs。5412、CTCs553CTCs。CTCsctDNA·693·.DNA。CTCsctDNACRC、CRC、、。CTCsctDNACRC。1.J.20084618103-104.2.20J.200473214-217.3Morris-HardemanD.CirculatingcancercellsJ.AnnOncol201021795-100.4MouliereFThierryAR.Theimportanceofexaminingthepropor-tionofcirculatingDNAoriginatingfromtumormicroenvironmentandnormalcellsincolorectalcancerpatientsJ.ExpertOpinBiolTher2012121209-215.5Alix-PanabieresCPantelK.CirculatingtumorcellsLiquidbi-opsyofcancerJ.ClinChem2013591110-118.6Alix-PanabieresCPantelKSehwarzenbachH.CirculatingtumorcellsandcirculatingtumorDNAJ.AnnRevMed201263199-215.7TamWLWeinbergRA.Theepigeneticofepithelial-mesenchymalplasticityincancerJ.NatureMed201319111438-1449.8HouHW.IsolationandretrievalofcirculatingtumorcellsusingcentrifugalforcesJ.SciRep201331259.9SollierE.Size-selectivecollectionofcirculatingtumorcellsusingVortexTechnologyJ.LabChip201414163-77.10HoeppenerAESwennenhuisJFTerstappenLW.Immunomagnet-icseparationtechnologiesJ.RecentResultsCancerRes2O121951243-48.11ChenJLiJSunY.Microfluidicapproachesforcancercellde-tectioncharacterizationandseparationJ.LabChip201212101753-1767.12SunJLiMLiuCetal.Doublespiralmicrochannelforlabel-freetumorcellseparationandenrichmentJ.LabChip201212203952-3960.13FusiALiuZKummerlenVetal.Expressionofchemokinerecep-torsoncirculatingtumorcellsinpatientswithsolidtumorsJ.JTransMed20121052-58.14CoumansFAVanDGBeckMetal.Filtercharacteristicsinflu-encingcirculatingtumorcellsenrichmentfromwholebloodJ.PLoSONE20138e61770.15Alix-PanabieresC.EPISPOTassayDetectionofviableDTCs/CTCsinsolidtumorpatientsJ.RecentResultsCancerRes201219569-76.16XuTLuBTaiYCetal.Cancerdetectionplatformwhichmeas-urestelomeraseactivityfromlivecirculatingtumorcellscapturedonmicrofiherJ.CancerRes201070166420-6426.17HughesADMattisonJWesternLTetal.Microtubedeviceforse-lecting-mediatedcaptureofviablecirculatingtumorcellsfrombloodJ.ClinChem2012585846-853.18HeitzerE.Complextumorgenomesinferredfromsinglecirculatingtumorcellsbyarray-CGHandnext-generationsequencingJ.CancerRes201373102965-2975.19GormallyE.CirculatingfreeDNAinplasmaorserumasbiomarkerofcarcinogenesisPracticalaspectsandbiologicalsignificanceJ.MutatRes20076352/3105-117.20ChristophKSabineW.Animprovedmethodfortheisolationoffree-circulatingplasmaDNAandcell-freeDNAfromotherbodyflu-idsJ.AnnNYAcadSci200811371135-139.21SchwarzenbachHHoonDSPantelK.Cell-freenucleicacidsasbiomarkersincancerpatientsJ.NatRevCancer2011116426-437.22FongSLZhangJTLimCKetal.Comparisonof7methodsforextractingcell-freeDNAfromserumsamplesofc
本文标题:结直肠癌循环肿瘤细胞及DNA的研究进展-吴康
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