七种木蓝属植物的染色体研究

2011294417～422PlantScienceJournalDOI10．3724/SP．J．1142．2011．40417121*1211．6100412．100049LeguminosaeIndigoferaL．7IndigoferaamblyanthaCraib、I．bungeanaWalp．、I．delavayiFranch．、I．scabridaDunn、I．szechuensisCraib、I．silvestriiPamp．、I．zollingerianaMiq．。2n=4x=3262n=2x=16。2A51A。。。、、、。Q942A2095-0837201104-0417-06ChromosomalStudiesonSevenSpeciesofIndigoferaL．ZHENGXia12GAOXin-Fen1*XUBo12TUWei-Guo11．EcologicalRestorationandBiodiversityConservationKeyLaboratoryofSichuanProvinceChengduInstituteofBiologyChineseAcademyofSciencesChengdu610041China2．GraduateUniversityofChineseAcademyofSciencesBeijing100049ChinaAbstractThechromosomenumberandkaryotypeofsevenspeciesofIndigoferaL．werestud-iedbyapplyingtheroottipsquashtechnique．Resultsshowthatsixspecieswerediploid2n=2x=16whileIndigoferazollingerianaMiq．wastetraploid2n=2x=32．ThekaryotypesofI．zollingerianaMiq．andI．silvestriiPamp．belongedto2Awhiletheotherswere1A．Thekaryologicaldifferencebetweenspecieswassmall．Mostchromosomesinthetestedsevenspeciesweremetacentric．ThekaryomorphologyofI．delavayiFranch．I．scabridaDunnI．sil-vestriiPamp．I．szechuensisCraibandI．zollingerianaMiq．arereportedhereforthefirsttime．KeywordsIndigoferaChromosomenumberKaryotypeIndigoferaL．Legumino-saeFaboideae750。794321。、、、、、。、、23。、、、、、3。4。。7、、、。2010-07-052010-09-03。1984－。*[e-mail]。11．17IndigoferaL．。1CDBI。1．22～4h25℃1～2cm0.002mol/L8-2h4℃1～2h95%∶=3∶14～6h1mol/L60℃5～7min。1．3530。5。Steb-bins6。As．K%Arano7。Levan8。272。2．12n=161A2a。2n=2x=16mm。。5.47～6.921.27As．K%=/×100%57.141A。2n=169102n=4811。2．22n=161B2b。2n=2x=14m+2smmsm。。5.12～7.691.3256.561A。2n=161AK2n=2x=16=14m+2sm1213。2．32n=161C2c。2n=2x=14m+2sm。。4.71～7.731.6458.441A。2．42n=161D2d。2n=2x=12m+4sm。。5.46～8.071.4859.391A。1Table1TestedmaterialsSpeciesLocalityHabitatVoucherI．amblyanthaCraibMaoxianSichuanMoisturevalley、08-11-1B．XuX．SunI．bungeanaWalp．LudingSichuanDryvalleyofDaduhe、07-11-8B．XuW．G．TuI．delavayiFranch．MuliSichuanValleyofBaidiaotown、07-11-12B．XuW．G．TuI．scabridaDunnMuliSichuanValleyofJinshariver、07-11-11B．XuW．G．TuI．silvestriiPamp．LudingSichuanSlopeofErlangshan、07-11-9B．XuW．G．TuI．szechuensisCraibMaoxianSichuanValleyofMinjiang、08-11-3B．XuW．G．TuI．zollingerianaMiq．MyitkyinMyanmarRoadside588AyeyarwadyExp．5888142927Table2KaryotypeparametersofthesevenspeciesofIndigoferaNo．I．amblyantha%RLARPCNo．I．bungeana%RLARPC12．79+4．13=6．921．48m13．59+4．10=7．691．14m23．24+3．68=6．921．14m22．87+3．69=6．561．29m32．57+3．91=6．481．52m32．77+3．59=6．361．30m42．68+3．57=6．251．33m42．77+3．48=6．251．26m52．68+3．57=6．251．33m52．77+3．48=6．251．26m62．57+3．46=6．031．35m62．15+3．79=5．941．76sm72．46+3．24=5．701．32m72．36+3．48=5．841．48m82．46+3．01=5．471．23m82．46+2．66=5．121．08mNo．I．silvestrii%RLARPCNo．I．scabrida%RLARPC13．51+4．22=7．731．20m13．38+4．69=8．071．39m23．18+4．50=7．681．41m22．40+4．48=6．881．86sm32．25+4．17=6．421．85sm32．29+4．15=6．441．81sm42．74+3．62=6．361．32m42．51+3．49=6．001．39m52．41+3．84=6．251．59m52．40+3．38=5．781．41m62．41+3．18=5．591．32m62．40+3．28=5．681．36m72．30+2．96=5．261．29m72．51+3．17=5．681．26m81．97+2．74=4．711．39m82．40+3．06=5．461．27mNo．I．delavayi%RLARPCNo．I．szechuensis%RLARPC12．64+4．28=6．921．62m13．62+4．24=7．861．17m21．91+4．92=6．832．57sm22．85+4．08=6．931．43m32．91+3．92=6．831．34m32．47+3．85=6．321．56m42．82+3．83=6．651．35m42．39+3．78=6．171．58m52．73+3．73=6．461．37m52．47+3．47=5．941．41m62．64+3．46=6．101．31m62．85+3．08=5．931．08m72．37+2．82=5．191．19m72．23+3．47=5．701．55m82．00+3．01=5．011．50m81．93+3．24=5．171．68mNo．I．zollingeriana%RLARPCNo．I．zollingeriana%RLARPC11．76+2．35=4．111．33m91．30+1．76=3．061．35m21．69+2．41=4．101．42m100．98+1．96=2．942．00sm31．37+2．15=3．521．57m111．24+1．50=2．741．21m41．43+2．09=3．521．45m121．24+1．37=2．611．11m51．50+1．96=3．461．30m131．17+1．37=2．541．17m61．43+2．02=3．451．41m141．11+1．43=2．541．29m71．63+1．76=3．391．08m151．04+1．43=2．471．38m81．17+2．02=3．191．72sm161．04+1．30=2．341．25mNotesRL－Relativelengthshort+long=totalAR－ArmratioPC－Positionofcentromere．9144A．I．amblyanthaB．I．bungeanaC．I．delavayiD．I．scabridaE．I．silvestriiF．I．szechuensisG．I．zollingeriana17Fig．1KaryotypeofthesevenspeciesinIndigofera024292．52n=161E2e。2n=2x=14m+2sm。。5.01～6.921.3859.932A。2．62n=161F2f。2n=2x=16m。。5.17～7.861.5258.401A。2．72n=321G2g。2n=4x=32=28m+4sm。。2.34～4.111.7557.762A。YehM．S142n=16。。a．I．amblyanthab．I．bungeanac．I．delavayid．I．scabridae．I．silvestriif．I．szechuensisg．I．zollingeriana27Fig．2KaryotypemodeofthesevenspeciesofIndigofera1244372n=3262n=16。。2A1A3。。2n=16x=89－17。37Table3KaryotypedataofthesevenspeciesofIndigoferaTaxonKaryotypeformulaAARRLRAs．K%TypeI．amblyanthaK2n=2X=14m+2sm1．341．2757．141AI．bungeanaK2n=2X=16m1．321．5056．561AI．delavayiK2n=2X=14m+2sm1．421．6458．441AI．scabridaK2n=2X=12m+4sm1．471．4859．391AI．silvestriiK2n=2X=14m+2sm1．531．3859．932AI．szechuensisK2n=2X=16m1．431．5258．401AI．zollingerianaK2n=4X=28m+4sm1．381．7557．762ANotesAAR－Averagearmratiolong/shortRLR－Relativelengthratiolongest/shortest．AS．K%50AS．K%0。Stebbins18。。7。。1GaoXinFenBrianDS．Indigofera．FloraofChinaVol．10M．BeijingSciencePressSt．LouisMis-souriBotanicalGardenPress2009．2．M．199440293－310．3．J．19893161－177．4．J．2007164－66．5．J．198534297－302．6StebbinsGL．ChromosomalEvolutioninHigherPlantsM．Lo