晚孕期孕妇口服益生菌制剂对新生儿-省略--4-IFN-水平影响的临床研究-刘明颖

IL－4、IFN－γ①②①①110004R725.7A1001-4411201206-0923-03①②【】IL－4、IFN－γ。342121。ELISAIL－4IFN－γ。①P＞0.05②IL－4P＜0.05IFN－γP＞0.05IL－4/IFN－γP＜0.05。IL－4IFN－γ。【】IL－4IFN－γClinicalresearchoftheeffectoforaladministrationofprobioticproductsamongthepregnantwomenduringthethirdtrimesterofpregnancyonintestinalfloraandlev-elsofIL－4IFN－γinumbilicalcordbloodoftheneonatesLIUMing－YingLIHuiSHIZhanetal.DepartmentofGynecologyandObstetricsAffiliatedShengjingHospitalofChi-naMedicalUniversityShenyang110004LiaoningChina〔Abstract〕ObjectiveTodetectthechangesofintestinalfloraandlevelsofIL－4IFN－γinumbilicalcordbloodoftheneonatesbyoraladministrationofprobioticproductsamongthepregnantwomenduringthethirdtrimesterofpregnancyexploretheeffectofprobioticsonneonatalimmunity.MethodsThirty－fourpregnantwomenwhoadoptedelectivecesareansectionweredividedintoexperimentalgroupandcontrolgrouprandomly21pregnantwomeninexperimentalgroupweretreatedwithmilkpowdercontainingprobioticswhile21preg-nantwomenincontrolgroupweretreatedwithmilkpowderwithoutprobioticstheneonatalfeceswereobtainedforbacterialcultureunderaerobicenvironmentandanaerobicenvironmentrespectivelythenbacterialsmearswereperformedtoobservethechangesofintestinalfloraofneonates.AftercesareansectiontheumbilicalcordbloodsamplesofneonateswerecollectedthenserumsampleswereobtainedELISAwasusedtodetectthelevelsofIL－4andIFN－γinthetwogroups.ResultsTherewasnosignificantdifferenceinthechangesofintestinalfloraofneonatesbetweenthetwogroupsP＞0.05therewassignificantdifferenceinumbilicalcordserumlevelofIL－4betweenthetwogroupsP＜0.05buttherewasnosignificantdifferenceinumbilicalcordserumlevelofIFN－γP＞0.05therewassignificantdiffer-enceintheratioofIL－4/IFN－γbetweenthetwogroupsP＜0.05.ConclusionOraladministrationofprobioticproductsamongthepreg-nantwomenduringthethirdtrimesterofpregnancyhasnoobviousimpactonthechangesofintestinalfloraofneonatesbutafterIL－4andIFN－γdetectioninumbilicalcordofneonatesitisindicatedthatoraladministrationofprobioticproductsamongthepregnantwomenduringthethirdtrimesterofpregnancymayenhanceneonatalimmunityandpreventorreducetheoccurrenceofneonatalallergicdiseases.〔Keywords〕ProbioticsIL－4IFN－γUmbilicalcordbloodfecesoftheneonates。。。probiot-ics〔1〕。。。11.12007～2008、、34·329·IL－4、IFN－γ61×106cfu/g212121。25～373026～3629P＞0.05。。2730g～4200g3380g2580～3830g3320gP＞0.05。1.23ml20min3000/15min－20℃。。24～36h48～60h72h。1.3ELISAIL－4IFN－γ。BL。0.1gPBS10～108BL48h。。1.4SPSS15.0α=0.05tMann－WhitneyUIL－4IFN－γt。22.1。。24～36h213%211%P＞0.05960%1267%P＞0.051。36h。。2.2IL－4IFN－γELISAIL－4IFN－γIL－4P＜0.05IL－4IFN－γP=0.654IL－4/IFN－γP＜0.05。3。〔2〕。TH1/TH2TH1TH2。TH1/TH2。Th2IL－4BIgEIL－4BIgEIgE。IL－4IL－5、IL－6、IL－13BFcR－ImRNAIgE。IL－4CD23IgE。IL－4BTIL－4。IgEIL－4IL－4IFN－γIL－4/IFN－γIgE〔3〕。TH1、TH2。40〔45〕、〔6〕〔7〕。、TIL－10TGF－β〔89〕。TH1Th2。〔10〕。〔11〕。Krause。IL－4IFN－γIL－4TH1TH2TH1/TH2。。41.〔M〕.·429·2012272003822MarksGB.Environmentalfactorsandgene－environmentinteractionsintheaetiologyofasthma〔J〕.CliniExpPhrmacolPhysiol20063332853PengTHaoLMadriJAetal.RoleofC5inthedevelopmentofair-wayinflammationairwayhyperresponsivenessandongoingairwayre-sponse〔J〕.JClinInvest200511515904FernandezMFBorisSBarbesC.Probioticpropertiesofhumanlacto-bacillistrainstobeusedinthegastrointestinaltract〔J〕.JApplMicro-biol20039434495AnnukHShchepetovaJKullisaarEetal.Characterizationofintesti-nallactobacilliasputativeprobioticcandidates〔J〕.JApplMicrobiol20039434036PrakashSJonesLM.ArtificialcelltherapyNewstrategiesforthethera-peuticdeliveryoflivebacteria〔J〕.JBiomedBiotechnol20052005447SudoNYuXNAibaYetal.Anoralintroductionofintestinalbacte-riapreventsthedevelopmentofalong－termTh2－skewedimmunologi-calmemoryinducedbyneonatalantibiotictreatmentinmice〔J〕.ClinExpAllergy20023211128SistekDKellyRWickensKetal.Istheeffectofprobioticsonatopicdermatitisconfinedtofoodsensitizedchildren〔J〕.ClinExpAllergy20063656299LinHCSuBHChenACetal.Oralprobioticsreducetheincidenceandseverityofnecrotizingenterocolitisinverylowbirthweightinfants〔J〕.Pediatrics20051151110.〔J〕.20061412111.〔J〕.2007134632009-02-16〔〕200233R714.22+1B1001-4411201206-0925-03【】、、。110A、B。An=68。Bn=42。3～4HSG1～2。A85.29%B66.67%P＜0.05A86.76%B71.43%P＜0.05A83.82%B57.43%P＜0.01A1.47%B2.38%。。【】Valueofearlyhydrotubationforthepatientswithtubalpregnancyafterlaparoscop-icconservativesurgeryMALiLIMingLULi－Hua.DepartmentofGynecologyandObstetricstheSixthPeople＇sHospitalAffiliatedtoShang-haiJiaotongUniversityShanghai200233China〔Abstract〕ObjectiveToexploretheeffectofearlyhydrotubationafterlaparoscopicconservativesurgeryonpatencyrateoffallopi-antubesofaffectedsidepostoperativeintrauterinepregnancyrateandrecurrentectopicpregnancyrateamongthepatientswithtubalpreg-nancy.MethodsOnehundredandtenpatientswithtubalpregnancywhohadbirthdemandandreceivedlaparoscopicconservativesurgerywereselectedanddividedintogroupAandgroupBrandomly.Sixty－eightpatientsingroupAweretreatedwithhydrotubationafterthefirstmenstruation.Forty－twopatientsingroupBdidn＇treceivehydrotubationafterthefirstmenstruation.Allthepatientsreceivedhysterosalpin-gographyat3－4monthsafteroperationthesecondarypregnancysituationswerefollowedupat