噬菌体裂解酶的研究进展

20125255ChinJBiologicalsMay2012Vol.25No.5Bacteriophage、、。Bacteriophagelysins、。1.TwortD′Herelle191519171-2。RNA、RNA、DNADNA。。ssRNAssDNAdsDNA。———。3LysozymeN-acetylmuramoyl-L-alanineamidaseN-L-En－dopeptidases。N-C-3。。2.。。1。3.··122123【】。3、。。【】【】Q939.48【】A【】1004-5503201205-657-04ProgressinresearchonbacteriophagelysinsZHANGRui-an*,LIUJun,FENGShu-zhangCollegeofAnimalScienceandTechnology,JilinAgriculturalUni－versity,Changchun130118,China【Abstract】Bacteriophagelysinsarecellwalllyticenzymesexpressedinthelatephaseofbacterialinfection,withenzymeac－tivityandsubstratespecificity.Mostofbacteriophagescontainthegenesencodingthreekindsofcellwalllyticenzymes,i.e.lysozyme,N-acetylmuramoyl-L-alanineamidaseandendopeptidases.Thispaperreviewstherecombinationandapplicationofbacteriophagelysinsaswellastheprogressinresearchonrecombinantlysinsinrecentyears.【Keywords】Bacteriophage;Lysins;[e-mail]657··20125255ChinJBiologicalsMay2012Vol.25No.5。。。2。1Tab1.BacteriophagelysinsasfocusesofstudyintheworldbpLysA11554LysB11465Gene10491366E2767Cpl-110178Ejl9Ejh9PlyPSA87010Ply11884610Lysp95475311LysK12Ply18718873PlyG70213Hyb546514Lyb5125714LzA88215PlyC160816LySMP144617Schuch18。Vasala19LL-H。Kikkawa20PlyG、ELISAPlyGB。Nelson21C1。Djurkovic22Cpl-1Cpl-1。2004。。2Tab2.StudiesonapplicationofrecombinantlysinsintheworldLL-H19PlyG20Clysin24Cpl-12225Cpl2123PlyGBS26LysH527Ply362628PlyC1623CPL21pET28aBL21DE3DEAE97%。CPL21。24PCRClysinpET22bPCR、pET22b-ClysinBL21DE3γ、、。7PhiX174EpBV220O157:H7E。O157:H798.4%。6D29gene10-Gp10SDS-PAGEgene10D29。、。4.658··20125255ChinJBiologicalsMay2012Vol.25No.5、①②③④。2001Nelson21A。。。Cpl-1。Rodriguez-Ro－dríguez-Cerrato25Cpl-11h2000μgCpl-115minLg4.70CFU／mlLg2.00CFU／ml。Cpl-10%Cpl-124hCpl-180%。Cpl-1。PlyGBSCheng26PlyGBSA、B、C、GLD、E、N。PlyGBSBpH5.0。PlyGBSB。、、、。Obeso27LysH5pH7.037℃37℃4h。Zimmer28Ply3626N-C-。Ply3626。Hoopes16PlyC。PlyC20%100030min1μgPlyC108CFU／ml。PlyC。5.。2020。、、、。。。。。1TwortFW．Aninvestigationonthenatureofultra-microscopicvirusesJ．Lancet191518648141241-1246．2D＇HérelleF．SurunmicrobeinvisibleantagonistedesbacillesdysentériquesJ．CRAcadSciParis1917165373-375．3FischettiVA．BacteriophagelysinsaseffectiveantibacterialsJ．CurrOpiMicrobiol2008115393-400．4CatalaoMJMilhoCGilFetal．Asecondendolysingeneisfullyembeddedin-framewiththelysAgeneofmycobacteriophageMs6J．PLoSONE2011661-12．5GilFGrzegorzewiczAECatalaoMJetal．MycobacteriophageMs6LysBspecificallytargetstheoutermembraneofMycobacteri－umsmegmatisJ．Microbiology2010156Pt51497-1504．659··20125255ChinJBiologicalsMay2012Vol.25No.56．D29J．2009332113-116．7．O157J．2010267672-675．8ReschGMoreillonPFischettiVA．PEGylatingabacteriophageendolysininhibitsitsbactericidalactivityJ．AMBExpress20111129-33．9MartínezVGarcíaPGarcíalJLetal．Controlledautolysisfacil－itatesthepolyhydroxyalkanoaterecoveryinPseudomonasputidaKT2440J．MicrobBiotechnol201144533-547．10SchmelcherMTchangVSLoessnerMJ．DomainshufflingandmoduleengineeringofListeriaphageendolysinsforenhancedlyt－icactivityandbindingaffinityJ．MicrobBiotechnol201145651-662．11PaulVDSundarrajanSRajagopalanSSetal．Lysis-deficientphagesasnoveltherapeuticagentsforcontrollingbacterialinfec－tionJ．BMCMicrobiol2011111195-204．12BeckerSCDongSLBakerJRetal．LysKCHAPendopepti－dasedomainisrequiredforlysisofliveStaphylococcalcellsJ．FEMSMicrobiolLett2009294152-60．13SainathraoSMohanKVKAtreyaC．Gamma-phagelysinPlyGsequence-basedsyntheticpeptidescoupledwithQdot-nanocrys－talsareusefulfordevelopingdetectionmethodsforBacillusan－thracisbyusingitssurrogatesB．anthracis-SterneandB．cereus-4342J．BMCBiotechnol2009967-73．14WangSKongJZhangX．Identificationandcharacterizationofthetwo-componentcelllysiscassetteencodedbytemperatebac－teriophageφPYB5ofLactobacillusfermentumJ．JApplMicro－biol200810561939-1944．15．、D．2009．16HoopesJTStarkCJKimHAetal．UseofabacteriophagelysinPlyCasanenzymedisinfectantagainstStreptococcuse－quiJ．ApplEnvironMicrobiol20097551388-1394．17．D．2011．18SchuchRNelsonDFischettiVA．AbacteriolyticagentthatdetectsandkillsBacillusanthracisJ．Nature20024186900884-889．19VasalaAVaLkkllaMCaldenterJetal．Geneticandbio－chemicalcharacterizationoftheLactobacillusdelbrueckiisubsp．lactisbacteriophageLL-HlysinJ．ApplEnvironMicrobiol199561114004-4011．20KikkawaHFujinamiYSuzukiSetal．Identificationoftheaminoacidresiduescriticalforspecificbindingofthebacteri－olyticenzymeofgamma-phagePlyGtoBacillusanthracisJ．BiochemBiophysResCommun20073633531-535．21NelsonDLoomisLFischettiVA．PreventionandeliminationofupperrespiratorycolonizationofmicebygroupAStreptococcibyusingabacteriophagelyticenzymeJ．ProcNatlAcadSciUSA20019874107-4112．22DjurkovicSLoefflerJuttaMFischettiVA．SynergistickillingofStreptococcuspneumoniaewiththebacteriophagelyticenzymeCpl-1andpenicillinorgentamicindependsonthelevelofpeni－cillinresistanceJ．AntimicrobAgentsChemother20054931225-1228．23．CPL21、J．2010102139-142．24．J．2005212216-219．25Rodríguez-CerratoVGarcíaPDelPradoGetal．Invitroin－teractionsofLytAthemajorpneumococcalautolysin．withtwobacteriophagelyticenzymesCpl-1andPalcefotaximeandmoxifloxacinagainstantibiotic-susceptibleand-res