植物病害的分子检测技术及其应用

ATEPCR。DNARFLP、RAPD、SSRPCRDNA。1PCRPCR2080、、、[1]。PCRDNA。PCR。PCR。RFLPAFLPRAPDDNASSR。PCR50[2]。DNA、、、、、、、、、DNA。ForsteCroft62RFLPHamelin、JonesCochilioboluscarbonumHerreroPhythiumaphanidexmatum、、RAPD。AFLP、、、、、AFLP。rDNAITS、、ITSPCR[2]。Nazar[3]verticilliumspp.ITSPCRV.albo-a-trumV.dahliae。2PCR2010-12-0803080120060110801984－。PCRPCR。PCRS41-30A1673-887X201101-0046-03doi10.3969/j.issn.1673-887X.2011.01.00146AGRICULTURALTECHNOLOGY&EQUIPMENT2011.01B206PCRPCR、PCR、PCR-、PCR、DNA[4]。2.1RT-PCRReversetranscriptionPCRRT-PCR[5]RT-PCR、3RT-PCR10。2.2IC-PCRPCRImmunocapture-PCRIC-PCRPCR。2.3PCR-SSCPPCR-PCRsingle-strandconforma-tionalpolymorphismPCR-SSCP、、。[6]BNYwRNA212BNYw4。2.4RAPDRAPD1990WilliamsWelshMoClel-land[7]。、RAPD-PCR[8]。2.5RFLPRestrictionfragmentlengthpolymorphismRFLP2080DNA、。RFLP。RFLP、DNA[9]。、PCR-RFLP136426427366657%262740.8%142.2%[10].2.6PCRPCRRealtimefluorescentPCRPCRPCR、、、。PCR、、。[11]。2.7SSRSSRDNAmicrosatelliteDNA,1～6100～200bp.DNA。SSRSSR[12]。2.8ISSRISSRZietkiewicz[13]。Tsumuraetal.,1996;Fang&Roose,1997PCR。ISSR[13]。2.9DNADNAmicroarraycDNADNA。LievensDNA、、、[14]。DNA。3PCR、。,、(5047ATE[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]47、PCR。、、、,[15]。123456789101112131415JoanMHRoyFrench.Thechainreactionandplantdiseasediagnosis[J].Ann.Rev.Phytopathol19933181-109．.[D]...2003.VolossioukT,RobbEJ,NazarRN.DirectDNAextractionforPCR-me-diatedassayofsoilorganisms.AppliedandEnviromentalMicrobiology,1995,61(11):3972-3976..PCR[J].2004,204112-115.,,,.RT-PCR、SDS-PAGE[J].200124424-28．.[J].,1957,7(4):467-472..CydiapomonellaL[D].200814-28..[J].19961011-7．FranckP,ReyesM,OlivaresJ,etal.Geneticdifferentiationinthe-codlingmoth:comparisonbetweenmicrosatlliteandinsecticidere-sistantmarkers.MolecularEcology,2007(16):3554-3564.,.[J].2008597.,,.[J].2008344111-114.HagleyEAC．Effectoflowtemperatureonmortalityofhibernat-ingcodlingmothlarvaeandfecundityofsurvivingadults．CanadaEntomo1．1977109l23-127．.[J].2009,352:102-103..[J].2009,364:343-348.,.[J].1994873730-735..[J].2008459-60..[J].20094118-120..[J].,1994,212:169-175...[J].,1981,4:12..[J]..1995940198-203..[J].,2007442:233-237..[J].2009364383-384.,.[J].,200929627-28..[J].,20093130-132..[J].,19851:39-42..[D].200912-25..[J].1982335..[J].,2009127-28..[J].20081731.Fuentes-ContrerasE,ReyesM,BarrosW,etalB.EvaluationofazinphosmethylresistanceandactivityofdetoxifyingenzymesincodlingmothLepidoptera:TortricidaefromCentralChile.Jour-nalofEconomicEntomology,2007100:551-556.．PCR-SSCPRNA2[J].200316-9.,,,.RAPD[J].2002173220-224.,,,.RAPD-PCR[J].20044374-79..rDNAITSPCR-RFLPs[J].,2007156545-546..M.2001,5-10..PCR[J].,2010,418148-155.,,,.(SSR)[J].2007,2:75-78..ISSR[D].2007.LIEVENSB,BROUWERM,VANACHTERACRC,etal.DesignanddevelopmentofaDNAarrayforrapiddetectionandidentificationofmultipletomatovascucarwiltpathogens[J].FEMSMicrobiologyletters,2003,223:113-122..[J].2010,382:597-599.50