常见细胞系细胞培养基应用选择及相关细胞培养资料

1常见细胞系细胞培养基应用选择选择培养基没有一定的标准，有几点建议可供参考：(1）建立某种细胞株所用的培养基应该是培养这种细胞首选的培养基。可以查阅参考文献，或在购买细胞株时咨询。(2)其它实验室惯用的培养基不妨一试，许多培养基可以适合多种细胞。(3)根据细胞株的特点、实验的需要来选择培养基。如小鼠细胞株多选RPMI1640。(4)用多种培养基培养目的细胞，观察其生长状态，可以用生长曲线、集落形成率等指标判断，根据实验结果选择最佳培养基，这是最客观的方法，但比较繁琐。细胞系细胞类型种组织培养基293成纤维细胞人胚胎肾MEM,10%热灭活马血清IMR-90成纤维细胞人肺MEM,10%胎牛血清和NEAAW1-38成纤维细胞人胚胎肺,3月妊娠MEM,10%胎牛血清A549上皮细胞人肺癌F-12K,10%胎牛血清A431上皮型人表皮细胞癌DMEM,10%胎牛血清BHL-100上皮细胞人乳房McCoy'5A,10%胎牛血清BeWo上皮滋养层人绒毛癌F-12K,15%胎牛血清Caco-2上皮细胞人结肠腺癌MEM,10%胎牛血清和NEAAChang上皮细胞人肝脏BME,10%小牛血清HCT-15上皮细胞人结肠直肠腺癌RPMI-1640,10%胎牛血清HeLa上皮细胞人子宫颈癌MEM,10%胎牛血清和NEAA(insuspension,S-MEM)HEp-G2上皮细胞人肝细胞癌MEM,10%胎牛血清和NEAAHEp-2上皮细胞人喉癌MEM,10%胎牛血清;或RPMI-1640,10%胎牛血清HT-1080上皮细胞人纤维肉瘤MEM,10%HI胎牛血清和NEAAHT-29上皮细胞人结肠腺癌McCoy'5A,10%胎牛血清JEG-2上皮细胞人绒毛膜癌MEM,10%胎牛血清MCF7上皮细胞人乳腺癌MEM,10%胎牛血清NEAA,10ug/ml胰岛素KB上皮细胞人口腔癌MEM,10%胎牛血清和NEAASaos-2上皮细胞人骨肉瘤McCoy'5A,15%胎牛血清WI-38上皮细胞人胚胎肺BME,10%胎牛血清WISH上皮细胞人羊膜BME,10%胎牛血清WS1人胚胎皮肤MEM,10%胎牛血清和NEAAHUVEC内皮细胞人脐带F-12K,10%胎牛血清和肝素盐100ug/mlEB-3淋巴样人Burkitt淋巴瘤RPMI-1640,10%胎牛血清Raji淋巴样人Burkitt淋巴瘤RPMI-1640,10%胎牛血清IM-9成淋巴细胞人骨髓瘤病人骨髓RPMI-1640,10%胎牛血清Daudi成淋巴细胞人淋巴瘤病人血液RPMI-1640,10%胎牛血清H9成淋巴细胞人T细胞淋巴瘤RPMI-1640,20%胎牛血清HL-60成淋巴细胞人早幼粒细胞白血病RPMI-1640,20%胎牛血清Jurkat成淋巴细胞人T细胞白血病RPMI-1640,10%胎牛血清K-562成淋巴细胞人骨髓性的白血病RPMI-1640,10%胎牛血清U937淋巴样人组织细胞淋巴瘤RPMI-1640,10%胎牛血清KG-1骨髓白细胞人红白血病病人骨髓IMDM,20%胎牛血清McCoy成纤维细胞小鼠未知MEM,10%胎牛血清BALB/3T3成纤维细胞小鼠胚胎DMEM,10%胎牛血清3T6成纤维细胞小鼠胚胎DMEM,10%胎牛血清A9成纤维细胞小鼠结缔组织DMEM+10%胎牛血清+6-硫鸟嘌呤（30μg/ml）+2,6-二氨基嘌呤（30μg/ml）AtT-20上皮细胞小鼠垂体肿瘤F-10,15%马血清和2.5%胎牛血清CloneM-3上皮细胞小鼠黑素瘤F-10,15%马血清和2.5%胎牛血清I-10上皮细胞小鼠睾丸癌F-10,15%马血清和2.5%胎牛血清Y-1上皮细胞小鼠肾上腺瘤F-10,15%马血清和2.5%胎牛血清2WEHI-3b类巨噬细胞小鼠骨髓单核细胞白血病DMEM,10%胎牛血清ES-D3胚胎型小鼠多能胚胎干细胞DMEM+15%胎牛血清+100μmol/L2-巯基丙醇F9胚胎型至内胚层小鼠睾丸畸胎癌DMEM,15%胎牛血清BHK-21成纤维细胞仓鼠肾GMEM,10%胎牛血清或MEM,10%胎牛血清和NEAAHaK上皮细胞仓鼠肾BME,10%小牛血清CHO-K1上皮细胞仓鼠卵巢F-12,10%胎牛血清AR42J.大鼠胰腺肿瘤Ham'sF-12K,20%胎牛血清BRL3A.大鼠肝Coon'sF-12K,5%胎牛血清Clone9上皮细胞大鼠肝脏F-12K,10%胎牛血清H4--Ⅱ-E-C3上皮样大鼠肝癌F-12K,10%胎牛血清`GH1上皮细胞大鼠垂体肿瘤F-10,15%马血清和2.5%胎牛血清GH3上皮细胞大鼠垂体肿瘤F-10,15%马血清和2.5%胎牛血清IEC-6上皮样大鼠正常小肠DMEM,5%胎牛血清,胰岛素0.1μg/mlL2上皮细胞大鼠肺F-12K,10%胎牛血清XC上皮细胞大鼠肉瘤MEM,10%胎牛血清和NEAALLC-WRC256上皮细胞大鼠癌199培养基,5%马血清Jensen成纤维细胞大鼠肉瘤McCoy's5A,5%胎牛血清Rat2(TK-)成纤维细胞样大鼠Rat2(TK-)克隆衍生DMEM,5%胎牛血清,30μg/mlBrdUPC12NGF诱导的神经表型细胞大鼠肾上腺嗜铬细胞RPMI-1640,10%胎牛血清L6大鼠大鼠骨骼肌成肌细胞DMEM,10%胎牛血清D-17上皮细胞狗骨肉瘤MEM,10%胎牛血清和NEAABT成纤维细胞牛鼻甲骨细胞MEM,10%胎牛血清和NEAAMARC-145猴猴肾MEM或DMEM,10%小牛血清CV-1成纤维细胞猴非洲绿猴肾MEM,10%胎牛血清COS-1成纤维细胞猴肾MEM,10%胎牛血清COS-3成纤维细胞猴肾MEM,10%胎牛血清COS-7成纤维细胞猴非洲绿猴肾MEM,10%胎牛血清Vero成纤维细胞猴非洲绿猴肾199培养基，5%胎牛血清B95-8成淋样绒猴EB病毒感染的白血病细胞RPMI-1640,10%胎牛血清CRFK上皮细胞猫肾MEM,10%胎牛血清和NEAA常用显色剂产品中文名产品英文名分子式分子量别名备注酸性铬兰KAcidChromeblueKC16H9N2Na3O12S3586.40邻氨基酚一对一磺酸钠偶氮-1,8-二羟基萘-3,6-二磺酸钠酸性铬深蓝AcidChromedarkblueC16H10N2Na2O9S2484.362-(2-羟基苯偶氮)-1,8-二羟基萘-3,6-二磺酸钠酸性品红AcidfuchsinC20H17N3Na2O9S3585.55酸性复红吖啶橙AcridineOrangeC17H20ClN3·ZnCl2盐酸3，6-双（二甲氨基）吖啶；碱性橙3吖啶黄AcridineyellowC15H15N3HCl273.76氮蒽黄；绿光碱性黄；2,6-二氨基-2.7-二甲基吖啶盐酸盐阿尔新蓝AlcianBlue8GXC36H88Cl4CuN16S41298.88阿尔新蓝8GX；阿利新蓝8GS；AlcianblueICI；NitionalfastblueBXM阿尔新绿2GXAlcianGreen2GX纱染绿2；Ingraingreen2茜素AlizarinC14H8O4240.211,2-二羟基蒽醌；1,2-DihydroxyanthraquinonepH5.8(黄)～7.2(紫)茜素络合指示剂AlizarincomplexoneC19H15NO8385.333-氨基甲基茜素-N,N-二乙酸；氟试剂，AlizarinFluorineblue茜素红SAlizarinredSC14H7NaO7S·H2O360.27茜素磺酸钠；SoliumalizarinsulphonatepH4.3(黄)～6.3(红)茜素紫AlizarinvioletC20H12O7364.31焦性没食子酚酞；Pyrogallolphthalein;GalleinpH3.8(棕黄)～6.6(玫红)碱兰6BAlkaliblue6BC37H34N4O4S626.71碱性兰6B;Alkalineskyblue鸡冠花红AmaranthC20H11N2Na3O10S3604.46苋菜红；对磺基萘偶氮－R－盐；蓝光酸性红；NaphtholredS氨基黑10BAmidoblack10BC22H14N6Na2O9S2616.494-萘胺黑10B;Naphthaleneblack10B苯胺兰(醇溶)AnilinebluealcholsolubleC32H28CIN3，C37H30CIN3490.02，552.09酒精兰；Spiritblue;Opalblue;Lightblue苯胺兰（水溶）AnilinebluewatersolubleC32H25N3Na2O9S3737.72可溶性蓝苯胺黄AnilineyellowC12H11N3197.24ρ-Aminoazobenzene；对氨基偶氮苯偶氮洋红BAzocarmineBC28H17N3Na2O9S3681.63偶氮胭脂红B；偶氮红BRosinduline2B偶氮洋红GAzocarmineGC28H18ClN3Na2O6S2638.02玫红对氮蒽；偶氮胭脂红G；偶氮洋红GX；Rosazine偶氮荧光桃红AzophloxineC18H13N3Na2O8S2509.42偶氮焰红；酸性大红；酸性红Acetylred；AmidonaphtholredG；AzogeramineBS金胺OAuramineOC17H22CIN3308.82碱性槐黄；Canaryyellow；奥黄；金丝雀黄天青ⅠAzureⅠC14H14ClN3S291.79亚甲天青Ⅰ；3-Amino-7-dimethylaminophenazathioniumchloride;蔚蓝Ⅰ天青ⅡAzureⅡ蔚蓝Ⅱ苯红紫4BBenzopurpurine4BC34H26N6Na2O6S2724.71Directred4B;Sultan4B;Fastscarlet;苯并红紫4B4猩红（水溶性）Biebrichscarlet(watersoluble)C22H14N4Na2O7S2556.47比布列西猩红（水溶性）；猩红B俾士麦粽RBismarckbrownRC21H26Cl2N8461.39俾士麦粽R；Vesuvine;Bismarckbrown53俾士麦粽YBismarckbrownYC18H18N8·2HCl419.32Vesuvine;BismarckbrownG玫瑰桃红RBordeauxRC20H12N2Na2O7S2502.42酸性枣红；萘偶氮R盐亮黄BrightyellowC26H18N4Na2O8S2624.55直接黄4；灿烂黄；二苯乙烯-2,2’-二磺酸钠-4,4’-双(1-偶氮)4-羟基苯；Brilliantyellow灿烂甲酚蓝BrilliantCresylblueC17H21ClN4O332.83亮甲苯酚蓝；煌焦油蓝；cresylblue；Brilliantblue灿烂丽春红5RBrilliantponceau5RC20H11N2Na3O10S3604.404-磺基-1-萘偶氮-2’-萘酚－6’，8’－二磺酸三钠盐溴酚兰BromophenolblueC19H10Br4O5S669.96四溴苯酚磺酞pH3.0(黄)～4.6(紫)溴酚红BromophenolredC19H12Br2O5S512.17二溴苯酚磺酞；DibromophenolsulphophthaleinpH5.2(黄)～7.0(红)溴氯酚兰BromochlorophenolblueC19H10Br2Cl2O5S581.06二溴二氯苯酚磺酞pH3.2(黄)～4.8(紫)溴邻苯三酚红BromopyrogallalredC19H10Br2O8S·H2O576.17二溴邻苯三酚磺酞；溴焦性没食子酸红；Dibromopyrogallolsulfonphthalein溴百里香酚兰BromothymolBlueC27H28Br2O5S624.38溴麝香草酚蓝；3,3”-二溴麝香草酚磺酜酞pH6.0(黄)~7.6(兰)钙黄绿素CalceinC30H26N2O13622.553,3’-双（甲胺二