高效液相色谱法测定葡萄酒中柠檬酸含量

200939、0.99980.03mg/L93.8％～106.2％RSD3%、、。AbstractAreversed-phasehighperformanceliquidchromatographicmethodwasintroducedfordeterminingthecontentofcitricacidsinwinewithphosphateandmethanolsolutionasmobilephase.Theresultshowsthatthismethodtakesongoodlinearrelationitscorrelativeconnectionis0.9998thelowestdetectionconcentrationis0.03mg/Ltheaveragerecoveriesrangedfrom93.8%to106.2%withtherelativestandarddeviationislessthan3%.Thismethodissimpleness,speedinessandhighveracityandgoodfidelity.keywordscitricacidhighperformanceliquidchro-matographywine、、≤1.0g/L≤2.0g/L。、、。C18、。11.1LC-10ATvpSPD-10AvpKQ5200DEPHS-3CAP-9925AB104-S-。1.2、、、、、。。1.31.3.11%10mL1000mL。*030012Determinationofcitricacidscontentinwinebyhighperformanceliquidchromatography*19752003。2009-07-20ZHANGSu-juan*(NationalFarmProductsandLiquorQualitySupervisionandInspectionCenter,ShanxiProvinceFoodstuffQualitySupervisionandInspectionCenterTaiyuan030012China)··≤≤≤≤≤≤≤≤≤≤≤≤≤≤≤≤≤≤FOODENGINEERING48200939g/LRSD％1.000g/LmLg/L％10.2102.982.00.28695.010.00.60097.521.4542.572.01.53196.210.01.85399.830.8271.652.00.90293.810.01.245104.541.7652.032.01.850106.210.02.15998.650.9681.552.01.04798.810.01.374101.550mL1%1000mL0.45μm、。0.02mol/LpH2.92.72g1000mLpH2.90.45μm、。1.3.20.10g0.0001g0.01mol/L100mL1.000g/L。1.410.00mL20℃100mL0.45μm。1.5C18150mm×4.6mmi.d5μm25℃1.0mL/min214nm10μL。22.10.01mol/L0.05mol/L0.10mol/L0.20mol/L0.40mol/L0.80g/L。0.45μm。2.2、。0.02mol/LpH=2.9。0.02mol/LpH=2.9、pH。。。2.38.78min。1。12.46Y=541498x0.05g/L～1.00g/Lr2=0.99979。2.5565RSD510.0mL25mL1.000g/L2.0mL、10mL。。51。1n＝61RSD3.0％93.8%～106.2％。2.60.0100.0050.0000123456789101112t/minA/mAU5449200939、、、、、、。。、。2.3。、。。2.4、《》、、、。。。、、。2.5、、。。。。1.———J.2005216-18.2.J.2008240-41.0.40mg/mL、、、、、10。5%。30.03mg/L。100.10mg/L。30.99980.03mg/L93.8%～106.2％、、。1.J.200793821-23.2.、J.2006731-34.3.J.20063(27)179-180.4.、J.2004244458-460.!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!4954