限制性内切酶兼容粘性末端Compatible-Cohesive-Ends

限制性内切酶兼容粘性末端CompatibleCohesiveEndsmatinldaEnzymeLigatedToRecleavedByAcc65I(G/GTACC)BanI(G/GTACC)Acc65I,BanI,KpnI,NlaIV,RsaIBsiWI,BsrGIRsaIAccI(GT/CGAC)AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--(GT/CGAC)ClaI,BstBI,TaqαITaqαIAciI(C/CGC)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqαI--BsaHI(GR/CGCC),HinP1I,NarIAciIHpaIIHpaIIAclI(AA/CGTT)AccI(GT/CGAC),AciI,ClaI,BstBI,HinP1I,HpaII,NarI,TaqαI--AgeI(A/CCGGT)AvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWI,BspEIBsaWI,HpaIIBsrFI(A/CCGGT),SgrAI(CA/CCGGTG)AgeI,BsaWI,BsrFI,HpaIINgoMIVBsrFI,HpaIIApaI(GGGCC/C)BanII(GGGCC/C),Bsp1286I(GGGCC/C)ApaI,BanII,Bsp120I,Bsp1286I,HaeIII,NlaIV,Sau96IApaLI(G/TGCAC)SfcI(C/TGCAG)BsgIApoI(A/AATTY)EcoRIApoI,Tsp509I(G/AATTY)EcoRIApoI,EcoRI,Tsp509I(R/AATTY)MfeI,Tsp509ITsp509IAscI(GG/CGCGCC)AflIII(A/CGCGT),MluIBstUI,HhaIBssHIIBssHII,BstUI,Cac8I,HhaIAseI(AT/TAAT)BfaI,Csp6I,NdeI--MseIMseIEnzymeLigatedToRecleavedByAsiSI(GCGAT/CGC)BsiEI(CGAT/CG)DpnII,PvuIPacIMseIPvuIDpnII,PvuIAvaI(C/CCGGG)AgeI,BsaWI,BspEI,BsrFI(R/CCGGY),NgoMIV,SgrAI(CR/CCGGYG)HpaII,NciI,ScrFI(C/TCGAG)XhoIAvaI,TaqαI,XhoI(C/TCGAG)SalITaqαI(C/CCGGG)XmaIAvaI,BsaJI,HpaII,NciI,ScrFI,SmaIAvaII(G/GWCC)PpuMI(RG/GACCY)AvaII,NlaIV,Sau96IRsrIIAvaII,Sau96IPpuMI(RG/GTCCY)AvaII,BsmFI,NlaIV,Sau96IAvrII(C/CTAGG)NheI,SpeI,XbaIBfaIStyI(C/CTAGG)AvrII,BfaI,BsaJI,StyIBamHI(G/GATCC)BclI,DpnIIAlwI,DpnIIBglII,BstYI(R/GATCY)AlwI,BstYI,DpnIIBstYI(G/GATCC)AlwI,BamHI,BstYI,DpnII,NlaIVBanI(G/GTACC)Acc65IAcc65I,BanI,KpnI,NlaIV,RsaI(G/GCGCC)KasIBanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIV(G/GTACC)BsiWI,BsrGIRsaIBanII(GGGCC/C)ApaI,Bsp1286I(GGGCC/C)ApaI,BanII,Bsp1286I,HaeIII,NlaIV,Sau96I(GAGCT/C)Bsp1286I(GAGCT/C),SacIAluI,BanII,BsiHKAI,Bsp1286I,SacIBclI(T/GATCA)BamHI,BstYI(R/GATCY)AlwI,DpnIIBglII,MboIIDpnIIEnzymeLigatedToRecleavedByBfaI(C/TAG)AseI,Csp6I,MseI,NdeI--BglII(A/GATCT)BamHI,BstYI(R/GATCY)AlwI,BstYI,DpnIIBclI,DpnIIDpnIIBsaHI(GR/CGYC)AccI(GT/CGAC),ClaI,BstBI,TaqαI--(GA/CGYC)AciI,HinP1IHgaI(GG/CGYC)AciI,HinP1IHhaI(GG/CGYC)HpaIIAciI(GA/CGYC)NarIBsaHI,HgaI(GG/CGYC)NarIBanI,BsaHI,HaeII,HhaI,NarI,NlaIVBsaWI(W/CCGGW)AgeI,BsrFI(R/CCGGY),SgrAI(CR/CCGGYG)AgeI,BsaWI,BsrFI,HpaIIAvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBspEIBsaWI,BspEI,HpaIIBsrFI(R/CCGGY),NgoMIVBsrFI,HpaIINgoMIVHpaIIBsiEI(CGAT/CG)PacIMseI(CGAT/CG)PvuIBsiEI,DpnII,PvuI(CGGC/CG)SacIIAciIBsiHKAI(GTGCA/C)Bsp1286I(GTGCA/C)BsiHKAI,Bsp1286IBsp1286I(GAGCA/C)BsiHKAI,Bsp1286IBsp1286I(GAGCT/C),SacIAluI,BanII,BsiHKAI,Bsp1286I,SacINsiI--PstI,SbfIBsgIBsiWI(C/GTACG)Acc65I,BanI(G/GTACC),BsrGIRsaIEnzymeLigatedToRecleavedByBsp1286I(GGGCC/C)ApaI,BanII(GGGCC/C)ApaI,BanII,Bsp1286I,HaeIII,NlaIV,Sau96I(GTGCA/C)BsiHKAIApaLI,BsiHKAI,Bsp1286I(GGGCC/C)BanII(GGGCC/C)BanII,Bsp1286I(GAGCT/C)BanII(GAGCT/C),BsiHKAI,SacIAluI,BanII,BsiHKAI,Bsp1286I,SacI(GWGCW/C)BsiHKAIBsiHKAI,Bsp1286I(GTGCA/C)NsiI--(GTGCA/C)PstI,SbfIBsgIBspEI(T/CCGGA)AgeI,BsaWI,BsrFI(R/CCGGY),SgrAI(CR/CCGGYG)BsaWI,HpaIIAvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWIBsaWI,BspEI,HpaIIBsrFI(R/CCGGY),NgoMIVHpaIIBspHI(T/CATGA)FatI,NcoI,PciIFatI,NlaIIIBsrFI(A/CCGGY)AgeI,BsaWIAgeI,BsaWI,BsrFI,HpaII(G/CCGGY)AgeI,BsaWI,NgoMIVBsrFI,HpaII(R/CCGGY)AvaI(C/CCGGG),XmaIHpaII,NciI,ScrFI(A/CCGGY)BsaWI,BspEIBsaWI,HpaII(R/CCGGY)BsaWI,BspEIHpaII(G/CCGGY)NgoMIVBsrFI,Cac8I,HpaII,NaeI(CR/CCGGYG)SgrAIBsrFI,HpaIIBsrGI(T/GTACA)Acc65I,BanI(G/GTACC),BsiWIRsaIBssHII(G/CGCGC)MluIBstUI,HhaIAscIBssHII,BstUI,Cac8I,HhaIBstBIAccI(GT/CGAC),ClaI,TaqITaqIEnzymeLigatedToRecleavedBy(TT/CGAA)AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--BstYI(A/GATCY)BamHI,BglIIAlwI,BstYI,DpnII(G/GATCY)BamHIAlwI,BamHI,BstYI,DpnII,NlaIV(R/GATCY)BclI,DpnIIDpnII(G/GATCY)BclI,DpnIIAlwI,DpnII(A/GATCY)BglIIBglII,BstYI,DpnIIClaI(AT/CGAT)AccI(GT/CGAC),BstBI,TaqITaqIAciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--DpnII/MboI/Sau3AI(/GATC)BamHI,BstYI(R/GATCC)AlwI,DpnIIBclI,BglII,BstYI(R/GATCY)DpnIIEaeI(Y/GGCCR)PspOMIHaeIII,Sau96I(C/GGCCR)EagIBsiEI,EaeI,EagI,HaeIII(T/GGCCR)EagIEaeI,HaeIII(C/GGCCR)NotIAciI,BsiEI,EaeI,EagI,Fnu4HI,HaeIII(T/GGCCR)NotIAciI,EaeI,Fnu4HI,HaeIIIEagI(C/GGCCG)PspOMIHaeIII,Sau96IEaeI(Y/GGCCR)EaeI,HaeIIIEaeI(C/GGCCG)BsiEI,EaeI,EagI,HaeIIINotIAciI,BsiEI,EaeI,EagI,Fnu4HI,HaeIIIEcoRI(G/AATTC)ApoI(G/AATTC)ApoI,EcoRI,Tsp509IApoI(R/AATTY)ApoI,Tsp509IMfeI,Tsp509ITsp509IFatI(/CATG)BspHI,NcoI,PciIFatI,NlaIIIEnzymeLigatedToRecleavedByHinP1I(G/CGC)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--AciI,BsaHI(GR/CGCC),NarIHhaIBsaHI(GR/CGTC)HgaIHpaIIAciIHpaII/MspI(C/CGG)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--AciI,BsaHI(GR/CGCC),HinP1I,NarIAciIKasI(G/GCGCC)BanI(G/GCGCC)BanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIVMfeI(C/AATTG)ApoI(R/ATTTY),EcoRI,Tsp509ITsp509IMluI(A/CGCGT)AscI,BssHIIBstUI,HhaIMseI(T/TAA)AseIMseIBfaI,Csp6I,NdeI--NarI(GG/CGCC)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqαI--AciI,HinP1IHhaIBsaHI(GR/CGCC)BanI,BsaHI,HaeII,HhaI,NarI,NlaIVBsaHI(GR/CGTC)BsaHI,HgaIHpaIIAciINcoI(C/CATGG)BspHI,FatI,PciIFatI,NlaIIINdeI(CA/TATG)AseI,BfaI,Csp6I,MseI--NgoMIV(G/CCGGC)AgeI,BsaWI,BsrFI(R/CCGGY),SgrAIBsrFI,HpaIIAvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWI,BspEIHpaIIBsrFI(R/CCGGC),SgrAIBsrFI,Cac8I,HpaII,NaeINheIAvrII,SpeI,StyI(C/CTAGG),XbaIBfaIEnzymeLigatedToRecleavedBy(