年产50000吨柠檬酸发酵车间设计

南阳理工学院本科生毕业设计学院（系）：生物与化学工程学院专业：生物工程学生：****指导教师：李慧星（讲师）完成日期2009年5月南阳理工学院本科生毕业设计年产50000吨柠檬酸发酵车间设计Designancitricacidfermentationworkshopplantwith50,000tonsannualoutput总计：毕业设计（论文）38页表格：7个插图：3幅南阳理工学院本科毕业设计年产50000吨柠檬酸发酵车间设计Designancitricacidfermentationworkshopplantwith50,000tonsannualoutput学院（系）：生物与化学工程学院专业：生物工程学生姓名：****学号：****指导教师（职称）：李慧星（讲师）评阅教师：完成日期：2010年2月~6月南阳理工学院NanyangInstituteofTechnology年产50000吨柠檬酸发酵车间设计I年产50000吨柠檬酸发酵车间设计生物工程专业田彬[摘要]本设计采用玉米原料发酵,只需将玉米磨粉,加水调浆,直接加入少量α－淀粉酶液化后,灭菌、冷却,即可接种发酵。制备柠檬酸一般采用晒干的玉米作为原料。玉米含水13%、淀粉70%左右、蛋白质6%左右。玉米原料中的蛋白质可作为氮源供菌体生长。玉米原料中含有铁、镁、钾、钙等的无机盐,选用的黑曲霉C0527对这些成分不敏感,故不必对原料做任何预处理。本设计采用好气液体深层发酵、钙盐法提取技术生产柠檬酸。这两种方法都是国内比较流行的生产方法，有着大量的实际经验，易于操作，风险小。通过工艺流程设计、工艺衡算、设备选型和车间布置设计，设计出年产50000吨柠檬酸发酵车间采用20个200m3发酵罐和7个30m3种子罐等。并依据生物工程工厂车间布置原则，对发酵车间进行合理布置，绘制了工艺流程图和车间布置图.完成了年产50000吨柠檬酸发酵车间的设计。工艺设计结果为柠檬酸的生产提供一定参考。[关键词]玉米深层好氧发酵黑曲霉柠檬酸设备设计及选型年产50000吨柠檬酸发酵车间设计IIDesignancitricacidfermentationworkshopplantwith50,000tonsannualoutputBio-engineeringMajorTianbinAbstractThedesignofrawmaterialsusedcornfermentation,simplycornmills,water-paste,addingasmallamountofdirectα-amylaseliquefaction,sterilization,cooling,fermentationcanbevaccinated.Preparationofcitricacidnormallyusedasrawmaterialsdriedcorn.Cornmoisturecontentof13%,about70percentofstarch,proteinabout6percent.Cornrawmaterialsintheproteincanbeusedassourceofnitrogenforcellgrowth.Cornrawmaterialscontainingiron,magnesium,potassium,calciumofinorganicsalt,optionalblackAspergillusnigerC0527isnotsensitivetotheseingredients,itneednotdoanypretreatmentoftherawmaterials.Thedesignofagoodgasliquidsdeepfermentation,drycalciumcitrateextractiontechnologyproduction,moreoverintroducedthecitricacidfermentsintechnicalprocessandfermentativeprocesseachcontrolmainpoint.Thetwomethodsaremorepopulardomesticproductionmethods,hasagreatdealofpracticalexperience,easytooperate,therisksmall.Throughprocessdesign,processcrossoperator,equipmentselectionandfacilitylayoutdesigntotheannualoutputof50,000tonsofcitricacidfermentationplantby11fermenterswith550m3and4seedscanswith55m3.Andfactoryfloorlayoutbasedontheprinciplesofbiologicalengineering,thereasonablelayoutofthefermentationplant,renderingtheprocessflowdiagramandplantlayout.Designancitricacidfermentationworkshopplantwith50,000tonsannualoutput.Processdesignforthecitricacidproductionresultsprovidesomereference.Keywords:CornDeep-seatedaerobicfermentationAspergillusnigerCitricacidEquipmentDesignandSelection年产50000吨柠檬酸发酵车间设计III目录1．前言......................................................................................................................................11柠檬酸的介绍..................................................................................................................11.1.1柠檬酸的性质.....................................................................................................11.1.2柠檬酸的发酵机理.............................................................................................11.1.3柠檬酸的主要用途.............................................................................................31.2柠檬酸工业的历史和国内外产业现状.......................................................................41.2.1柠檬酸工业的历史.............................................................................................41.2.2世界柠檬酸产业现状.........................................................................................41.2.3我国柠檬酸产业现状.........................................................................................51.3我国柠檬酸工业展望...................................................................................................51.4本设计的意义和主要任务...........................................................................................61.4.1本设计的意义.....................................................................................................61.4.2本设计的主要任务.............................................................................................72生产工艺................................................................................................................................82.1生产方法.......................................................................................................................82.2工艺流程......................................................................................................................82.3操作工艺......................................................................................................................93工艺计算书..........................................................................................................................123.1物料衡算....................................................................................................................123.1.1工艺技术指标及基础数据..............................................................................123.1.2原料消耗计算..................................................................................................123.1.3发酵醪量的计算..............................................................................................133.1.4接种量................................................