植物DNA条形码技术的发展及应用_刘宇婧

201120174－8293No．24JournalofPlantResourcesandEnvironmentReviewArticleDNA11112①1．1000812．650204DNADNA、、DNA。DNA。DNA22DNAmatK、trnH－psbA、rbcLITSDNADNA、、/、、DNADNA。DNAQ789Q949A1674－7895201101－0074－09ProgressandapplicationofDNAbarcodingtechniqueinplantsLIUYu-jing1LIUYue1HUANGYao-jiang1LONGChun-lin12①1．CollegeofLifeandEnvironmentalSciencesMinzuUniversityofChinaBeijing100081China2．KunmingInstituteofBotanytheChineseAcademyofSciencesKunming650204ChinaJ．PlantResour．＆Environ．201120174－8293AbstractBasedonsummarizationandanalysisofdevelopmentprocessofDNAbarcodingtechniqueresearchprogressofDNAbarcodingtechniqueinplantsitsworkingprocessandanalysismethodinfluencingfactorsonitsidentificationaccuracyitsapplicationstatusanddisputeexistinginplanttaxonomicstudywerecomprehensivelyanalyzedanddescribedandfurtherdevelopmenttrendandapplicationprospectofDNAbarcodingtechniqueinplantswereproposed．BymeansofsomeresearchexamplesitwasindicatedthatcombiningmethodofDNAbarcodingtechniqueinplantsandtraditionalbotanicalknowledgecouldbetakenasoneofstudyingwaysforethnobotany．AndalsoitwassuggestedthatcommonDNAbarcodinginplantsmainlyweretwomodesofsinglefragmentandmultiplefragmentscombiningandbothofthemhadtheirrespectiveadvantagesanddisadvantages．CommonDNAsequencesincludedmatKtrnH-psbArbcLandITSetcbuttheyallhadacertainlimitation．DifferentstandardsofDNAbarcodinginplantsshouldbeselectedinordertodifferentapplicationaims．Influencingfactorsonitsidentificationaccuracyincludedtypeandnumberofspeciesconstructionmethodofphylogenetictreehybridizationandgeneintrogressionvarianceofspeciesorigintimeandvarianceofmolecularevolutionrate．CurrentfocusonDNAbarcodinginplantsishowtoselectsuitableDNAfragmentsandtoevaluatetheirvalues．KeywordsDNAbarcodingdevelopmentprogresstaxonomyplantspeciesidentificationethnobotany2010－08－16B08044“985”MUC－985O92441112F1985—DNA。①[e-mail]17015%。。1－3。DNADNAbarcoding1DNA、。DNA。COI1650bpDNACOICOI4－5。DNA6。DNA。DNA、、。DNA①DNADNADNADNADNA。②。③DNA。④DNA27。1DNA1．1DNA20032009DNA。200320、2“TaxonomyandDNA”“TaxonomyDNAandtheBarcodeofLife”。2004SloanSmithsonianInstitutionCBOLtheConsortiumfortheBarcodeofLife、、。2005CBOL、DNA1000DNAGenBankDNA。2007DNADNA。2009DNADNArbcLmatKDNAtrnH－psbAITSDNAITS2DNA。DNA8－10。DNA571DNA。UniversityofGuelph、Hebert“DNA”。HebertCICOI11113320Cnidaria98%0%～2%11．3%COI1650bpCOIDNA11－12。DNA611－15。COIDNADNADNA、DNA。DNA。2DNADNA。1．2DNA1．2．1DNANewmaster16－17①UPADNADNA②trnD－trnYtrnH－psbAtrnD－trnY③rbcL。UPADNAUPA618－19。KressEricksonrbcLDNAtrnH－psbA20。UmbelliferaeHeracleumL．PoaceaeGlyceriaR．Br．trnH－psbA21－22trnH－psbA。Lahaye238DNAOrchidaceaematKDNA。Fazekas6matK、trnH－psbA、rbcL、ITS1、UPA、rpoB、rpoC1、atpF－atpHpsbK－psbI3292251trnH－psbA。Newmaster24matK、trnH－psbArbcLAcaciaMill．。Song25rbcL、trnH－psbA、ndhJ、rpoB、rpoC1、accD、ycf5nrITSPolygonaceae38trnH－psbADNA。Hebert“DNA”DNA。75348006600ITS292．7%ITS2DNA26。DNAITS2、trnH－psbA、rbcL、rpoC1ycf5Rutaceae72192300ITS2ITS227ITS2AstragalusL．100%85．7%28。BetulaceaeAlnusMill．26131rbcL、matK、trnH－psbAITS10．0%、31．2%、63．6%79．6%29。67201．2．2DNADNAmatK、trnH－psbA、rbcLITSDNADNA。matK230。Lahaye23matK390F/1326R1667100%Fazekas650%matK。trnH－psbA312。trnH－psbADNA20。rbcL21632－33rbcLDNA20。ITS20DNA2634ITS2。ITS2ITS15．8S26SrRNAITS2DNA26－2735。ITSITS2ITSDNA262836。trnLUAAp6DNA。、、p6DNADNA37。rpoB2rpoC1。238。1．3DNADNA。Kress2005trnH－psbAITS34。Chase“”39。Newmaster17112。ChaserpoC1rpoBmatKrpoC1rpoBmatKmatKrpoC1matKtrnH－psbArpoC1matKtrnH－psbA30。2005KressErickson20rbcL、rpoC1、rpoB2、trnH－psbA、matKITS1rbcLtrnH－psbArpoB2、rpoC1、rbcL、matKtrnH－psbA。Fazekas6DNArbcLtrnH－psbA。7、CBOLrbcLmatKDNA2。2911DNA771DNA1DNArbcL、matK、ITStrnH－psbA、、。DNADNA。1．4DNADNADNADNA。Kress34Taberlet37DNA①②DNADNA③DNA700bpPCRDNA④DNA⑤DNA。Erickson40DNA①PCR90%②1DNA③DNA④DNA⑤。DNA。DNADNA39。2DNA2．1DNA。Lahaye23matK1000matKDNA2010Newmaster41DNABiophytumDC．3TripogonRoem．etSchult．1。DNA。2．2Jurado－Rivera38DNA。trnLPeltoschema4。DNA。2．3DNA。parataxonomist。、、、、、。。DNA。NewmasterDNA。2IrulasMalasars“Sunaipul”1informant。1。NewmasterDNA8720Sunaipul1TripogoncopeNewmaster。DNAethnotaxon42。NewmasterDNA。“”ethnobotanygenomics41。SabiaColebr．SabiaparvifloraWall．exRoxb．1、。trnH－psbA、rbcLmatK67433DNA0．00%～0．86%24．50%。DNA。2．4AIT2009Newmaster44AITautomatedidentificationtechnology1AIT、、、。AIT。3DNA3．1DNA、DNA、DNA、、、、、。。DNAMEGAPAUPK2Pkimura－2－parameterdistance、3。DNA3845－50。3．2DNA3．2．1DNA、PCR、DNAPCR、。DNA、、barcodinggapDNA14。3．2．2DNAGenBankClustalXDNAMANBioeditMEGA4．0bootstrap1000214。3．2．3DNA4DNA。BLAST。DNAqueryquery。、。。DNAK2Pbarcodinggap。971DNA。51。。DNA142352。DNAWilcoxonsignedranktests。4DNA4．13K2P、θ3。105DNA53。averageinterspecificdistance、θaveragethetaprimesmallestinterspecificdistance26513。4．2discretecharactermethoddistancemethod2。maximumparsimonymethodmaximumlikelihoodmethodUPGMAneighbor-joining。Lahaye23MPUPGMANJ。“”。4．3/DNA。4．4DNA。100DNA54－55。4．5100256。DNA。5DNAHebert“DNA”。COIDNA1157－58DNA5960。DNADNA。DNADNA。DNADNADNA。DNADNA。DNA0820。DNADNA4161－65。DNA、DNAAIT。、DNA、24。、DNA3。、。、、DNA。1．DNAⅠ．DNAJ．2008272303－306．2HollingsworthPMForrestLLSpougeJLetal．ADNAbarcodeforlandplantsJ．ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica2