MIQE指南

Job#08-0000Date00/00/08Round_______________BulletinCN5859ඇࡔ௨ݯྼႪඤ၍800-8205567࿄ਸཚں൶൩Ӛ:021-64264966࣌ᆓݡ࿚;նૂิంᅅბׂ೗!)ฉ࡛*!ᆶ၌ࠅິں኷ǖฉ࡛๨Ⴘࣹ൶ཀሃൃୟ333ࡽ༮ݧٷေ10୍01ڇᇮۉࣆǖ021-64260808دኈǖ021-64264988ᆰՊǖ200030ԛ৙ݴࠅິǖں኷ǖԛ৙๨࡛ۏ൶ኪؾୟ113ࡽᆀྪዐ႐702๪ۉࣆǖ010-82675748دኈǖ010-62529800ᆰՊǖ100086࠽ዝݴࠅິǖں኷ǖ࠽ዝ๨࣍๨۫ୟ403ࡽ࠽ዝࡔाۉጱٷေ1302-03๪ۉࣆǖ020-87322339دኈǖ020-87322332ᆰՊǖ510095࿴ࡲݴࠅິǖں኷ǖࢶԛู࿴ࡲ๨ࡲ੨൶঴ݣٷڢ634ࡽႎ๘হዐ႐Bፗ1202๪ۉࣆǖ027-83806255دኈǖ027-83806265ᆰՊǖ430032BulletinCN5859ඇࡔ௨ݯྼႪඤ၍800-8205567࿄ਸཚں൶൩Ӛ:021-64264966࣌ᆓݡ࿚;նૂิంᅅბׂ೗!)ฉ࡛*!ᆶ၌ࠅິں኷ǖฉ࡛๨Ⴘࣹ൶ཀሃൃୟ333ࡽ༮ݧٷေ10୍01ڇᇮۉࣆǖ021-64260808دኈǖ021-64264988ᆰՊǖ200030ԛ৙ݴࠅິǖں኷ǖԛ৙๨࡛ۏ൶ኪؾୟ113ࡽᆀྪዐ႐702๪ۉࣆǖ010-82675748دኈǖ010-62529800ᆰՊǖ100086࠽ዝݴࠅິǖں኷ǖ࠽ዝ๨࣍๨۫ୟ403ࡽ࠽ዝࡔाۉጱٷေ1302-03๪ۉࣆǖ020-87322339دኈǖ020-87322332ᆰՊǖ510095࿴ࡲݴࠅິǖں኷ǖࢶԛู࿴ࡲ๨ࡲ੨൶঴ݣٷڢ634ࡽႎ๘হዐ႐Bፗ1202๪ۉࣆǖ027-83806255دኈǖ027-83806265ᆰՊǖ430032参考文献BustinSAetal.(2009).TheMIQEguidelines:Minimuminformationforpublicationofquantitativereal-timePCRexperiments.ClinChem55,611-622.DenisovVetal.(2008).DevelopmentandvalidationofRQI:AnRNAqualityindicatorfortheExperionautomatedelectrophoresissystem.Bio-RadBulletin5761FleigeSandPfafflMW(2006).RNAintegrityandtheeffectonthereal-timeqRT-PCRperformance.MolAspectsMed27,126-139.GarsonJAetal.(2009).Unreliablereal-timePCRanalysisofhumanendogenousretrovirus-w(HERV-W)RNAexpressionandDNAcopynumberinmultiplesclerosis.AIDSResHumRetroviruses25,377-378;authorreply379-381.GingrichJetal.(2006).EffectofRNAdegradationonthedataqualityinquantitativePCRandmicroarrayexperiments.Bio-RadBulletin#5452GutierrezLetal.(2008).Thelackofasystematicvalidationofreferencegenes:Aseriouspitfallundervaluedinreversetranscription-polymerasechainreaction(RT-PCR)analysisinplants.PlantBiotechnolJ6,609-618.HellemansJetal.(2007).Qbaserelativequantificationframeworkandsoftwareformanagementandautomatedanalysisofreal-timequantitativePCRdata.GenomeBiology8,R19.HuggettJetal.(2005).Real-timeRT-PCRnormalization;strategiesandconsiderations.GenesImmun6,279-284.ImbeaudSetal.(2005).TowardsstandardizationofRNAqualityassessmentusinguser-independentclassifiersofmicrocapillaryelectrophoresistraces.NucleicAcidsRes33,e56.LefeverSetal.(2009).RDML:Structuredlanguageandreportingguidelinesforreal-timequantitativePCRdata.NucleicAcidsResAdvanceAccesspublishedFebruary17,2009(accessedMay21,2009).Pfaffl,M.(2001).Anewmathematicalmodelforrelativequantificationinreal-timeRT-PCR.NucleicAcidsRes29,e45.RozenSandSkaletskyHJ(2000).Primer3onthe(Totowa,NJ:HumanaPress),pp.365-386.VandesompeleJetal.(2002).Accuratenormalizationofreal-timequantitativeRT-PCRdatabygeometricaveragingofmultipleinternalcontrolgenes.GenomeBiol3,research0034.1-0034.11ZukerM(2003).MFOLDwebserverfornucleicacidfoldingandhybridizationprediction.NucleicAcidsRes31,3406-3415.AcknowledgementsWearegratefultoDrJoVandesompeleforhisinsightfulcommentsandhelpfulcontributionstothispaper.Bio-Rad’sreal-timethermalcyclersarelicensedreal-timethermalcyclersunderApplera’sUnitedStatesPatent6,814,934B1foruseinresearch,humaninvitrodiagnostics,andallotherfieldsexceptveterinarydiagnostics.ThisproductiscoveredbyoneormoreofthefollowingU.S.patentsortheirforeigncounterpartsownedbyEppendorfAG:U.S.PatentNos.6,767,512and7,074,367.EvaGreenisatrademarkofBiotium,Inc.Bio-RadLaboratories,Inc.islicensedbyBiotium,Inc.tosellreagentscontainingEvaGreendyeforuseinreal-timePCR,forresearchpurposesonly.Practiceofthepatented5'NucleaseProcessrequiresalicensefromAppliedBiosystems.Thepurchaseoftheseproductsincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser’sowninternalresearchwhenusedwiththeseparatepurchaseofLicensedProbe.Nootherpatentrightsareconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedfromtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.CFXManagersoftwareisforusewithauthorizedreal-timeinstrumentorthermalcycler.Pleaseseeusermanualforcompletelicensingstatement.NanodropisatrademarkofThermoFisherScientific,Inc.Informationinthistechnotewascurrentasofthedateofwriting(2009)andnotnecessarilythedatethisversion(revA,2009)waspublished.Job#08-0000Date00/00/08Round_______________BulletinCN5859ඇࡔ௨ݯྼႪඤ၍800-8205567࿄ਸཚں൶൩Ӛ:021-64264966࣌ᆓݡ࿚;նૂิంᅅბׂ೗!)ฉ࡛*!ᆶ၌ࠅິں኷ǖฉ࡛๨Ⴘࣹ൶ཀሃൃୟ333ࡽ༮ݧٷေ10୍01ڇᇮۉࣆǖ021-64260808دኈǖ021-64264988ᆰՊǖ200030ԛ৙ݴࠅິǖں኷ǖԛ৙๨࡛ۏ൶ኪؾୟ113ࡽᆀྪዐ႐702๪ۉࣆǖ010-82675748دኈǖ010-62529800ᆰՊǖ100086࠽ዝݴࠅິǖں኷ǖ࠽ዝ๨࣍๨۫ୟ403ࡽ࠽ዝࡔाۉጱٷေ1302-03๪ۉࣆǖ020-87322339دኈǖ020-87322332ᆰՊǖ510095࿴ࡲݴࠅິǖں኷ǖࢶԛู࿴ࡲ๨ࡲ੨൶঴ݣٷڢ634ࡽႎ๘হዐ႐Bፗ1202๪ۉࣆǖ027-83806255دኈǖ027-83806265ᆰՊǖ430032BulletinCN5859ඇࡔ௨ݯྼႪඤ၍800-8205567࿄ਸཚں൶൩Ӛ:021-64264966࣌ᆓݡ࿚;նૂิంᅅბׂ೗!)ฉ࡛*!ᆶ၌ࠅິں኷ǖฉ࡛๨Ⴘࣹ൶ཀሃൃୟ333ࡽ༮ݧٷေ10୍01ڇᇮۉࣆǖ021-64260808دኈǖ021-64264988ᆰՊǖ200030ԛ৙ݴࠅິǖں኷ǖԛ৙๨࡛ۏ൶ኪؾୟ113ࡽᆀྪዐ႐702๪ۉࣆǖ010-82675748دኈǖ010-62529800ᆰՊǖ100086࠽ዝݴࠅິǖں኷ǖ࠽ዝ๨࣍๨۫ୟ403ࡽ࠽ዝࡔाۉጱٷေ1302-03๪ۉࣆǖ020-87322339دኈǖ020-87322332ᆰՊǖ510095࿴ࡲݴࠅິǖں኷ǖࢶԛู࿴ࡲ๨ࡲ੨൶঴ݣٷڢ634ࡽႎ๘হዐ႐Bፗ1202๪ۉࣆǖ027-83806255دኈǖ027-83806265ᆰՊǖ430032technote585