高效液相色谱法测定灵芝多糖含量

,2009,11(S1):65-67JournalofAgriculturalScienceandTechnology:2009208215;:2009210228:(CAFYRIF200708):,,,,,(,,100091):,,104,,0.6mL/min,,70,0.03g/L,Y=109779.6X-903.3r=0.9999,98%104%,RSD%=0.12(n=6),:;;;:TS207:A:100820864(2009)S120065203DeterminationofGanodermaLucidumPolysaccharidebyReversed2phaseHighPerformanceLiquidChromatographyHANZhen2tai,ZHAOYu2juan,LIUHui2wen,LIRu2hua(KeyLaboratoryofForestEcologicalEnvironmentofMinistryofForestry;ResearchInstituteofForestryEcology,EnvironmentandProtection,ChineseAcademyofForestry,Beijing100091,China)Abstract:Areversed2phasehighperformanceliquidchromatographicmethod(HPLC)forthedeterminationofganodermalucidumpolysaccharidewasdeveloped,usingSUGURPARK2(6.5300mm)astheanalysiscolumn,glucan10000asstandard,waterasmobilephaseataflowrateof0.6mL/min.Thedifferentialrefractometerdetectorwasusedandthecolumntemperaturewas70.Thelimitofdetectionwas0.03g/L.ThelinearregressionequationwasY=109779.6X-903.3,thecorrelationcoefficientr=0.9999.Themethodrecoveriesringfrom98%to104%.Relativestandarddeviationwas0.12%(n=6).Themethodissimply,stable,suitableforthedetermina2tionofganodermalucidumpolysaccharide.Keywords:highperformanceliquidchromatography;differentialrefractometerdetector;ganodermalucidum;polysaccharide,,,,,,,DNARNA,[1],2,,,,105,,,,,,,,,[2],,,,,,,,(),,104,,()11.1Waters244Waters410M5102000;104;,PurelabUltraGenetic(ELGALab2water)1.2:SUGURPARK2(6.5300mm);:;:0.6mL/min;:;:701.310425mg25mL,ddH2O,1.4,60,2.0g100mL,50mL,50mL,1h,,1.5(1.0g/L)0.05g/L0.10g/L0.20g/L0.40g/L0.80g/L1.00g/L,,20L,,,20L,RSD,,,,2.42.2,22.120L(1.0g/L),1,,,20L,2,6611,Y=109779.6X-903.3,r=0.9999,0.031.00g/L2.21,25,0103g/L1Table1Recoveriesanddetectionlimitsofthemethod.(%)Recoveries(%)(g/L)Detectionlimits(g/L)98%104%0.032Table2Precisionsofthemethod.Gradation(mAu)Peakarea(mAu)(X)Meanvalue(X)(Sx)Standarddeviation(Sx)(CV%)Coefficientofalienation(CV%)12187560221827653218560721834772624.30.124218151752182845621805653,,,[1],.[J].,1991,23(6):473-475,471.[2],,,.[J].,2001,2(7):644.76S1: