多糖的提取-分离纯化及分析鉴定方法研究

2712200912HENANSCIENCEVol.27No.12Dec.20092009-07-231102004010041965－.1004-3918200912-1524-06、121111.4500022.450002、..O629.12A、.1936Shear2050..70.、[1-3]、、、、、[4]、、.[5]21.1、、3[6-7]..、、.、、、、[8]、、.1．11．1．1.、.70%5h.、、、..[9]V∶V＝1∶2090℃2h23．5%.[10]1∶2080℃41．5h.4．25%..、.1．1．2.200912pH.KimikoAnno[11]→100g→1．310．17mol·L－1HClpH＝265～70℃1h→→3→NaOH→0．5L→→2→→→17．8g23．7%.[12]1g→→10000r/min15min→＋395％→→10000r/min15min→0．1mol·L－1.H＋..1．1．3[13].[14]70℃NaOHpH＝83、、Savage、、、DATE0．387%0．061%.[15]30%45℃90min48%..1．1．4...CO2TC＝304．6℃Tp＝7．38MPa8～40MPaCO2、.[16]CO2.[17].、、..1．21．2．1.、..[18]pH4．850℃60min40%50%.[19]60℃pH6．52%2h.20．32%..[20]0．15%40℃、pH4．53h20100℃、pH6．51h、、、19．9%.1．2．2、、、、pH.[21].、pH、50℃pH5．050min0．7%43．0%.[22]9、.30℃、81．2%、1525--2712.80．87%0．73%61．88%1．77%21．58%2．57%2～41%3．59%1．24%..、.1．31．3．1.[23]70℃600W30min.[24]400W1∶40gmLpH8．58minV∶V=5∶179．912mg/g..1．3．2[25][26]、..[27]1∶15、15min、60℃、420W32．30%.[28]7%.[29]3/430%..1．3．320～80kV/cm、、、、[30-32].、、1V..[33]、0．5％KOH20kV·cm－16μs55．59％.、、1．77..22．12．1．1SevageV∶V5∶14∶120～30min..Sevage..[34]1/4Seavg30min.2．1．210minSeavg1526--200912.2．1．3.5%～10%..[35]SeavgSeavg.SevagSevag、、.2．2、、、、、、DEAE－.2．2．13.50100200900mL/L.[36]10%30%45%65%5.2．2．2.[37]、、、DEAF－SepharosefastflowSephadexG－200SLPS.[38]DEAE－SepharoseF．FSepharoseCL－6BSephacryS－300CPS－1.2．2．3membraneseparationtechnologyMST、、.[40].[41]5．7％.33．1－、－、、、、、yaphe[42]、、、[43]、HPLC、、、[44]、、－、DNS[45]、、..、、、.3．2.、、、HLPC.HLPC[46].3．3.、、、、、、、、、HPLC、、、GPC[47]、MALDI－TOF－MS.3．43．4．1、、..、、、、、α－β－、1527--2712、、、、Smithterho[40]、[41]、、、、..3．4．2NMR2D－NMR13CNMR、[48].CD[49]CD.[50].4.、...1．J．200628298－100．2．J．200622121411－1412．3．J．200114616－8．4TanFengDengJun．AnalysisoftheconstituentsandantisenilefunctionofachyranthesbidentatapolysaccharidesJ．ActaBotanicaSinica2002447795-798．5．———J．2007450－51．6．J．200846119－122．7．J．2006273256－262．8．J．2007283186－188．9．J．200411127-129．10．J．2006278159－161．11KimikoAnno．IsolationandpurificationoffucoidanfrombrownseaweedpelvetiawrightiiJ．AgrBiolChem1966305495－499．12．J．2007289128－131．13．J．2001144264－268．14．J．20024158－60．15.J.2008331264－66．16．CO2J．1998299601－602.17．J．2003215821－823．18．J．2007284217－219．19．J．2000635-7．20．J．2002281045-48．21．J．2008351291－94．22．J．20081242－43．23．－J．200634121697－1701．24．J．2008439－42．25StahmannKPMonschauNSahmHetal．Structurepropertiesofnativeandsonicatedcinereanα－β－1－3－1－6－D－glu－canproducedbybotrytiscinereaJ．CarbohydrateResearch19952261115－128．26WeiLingyunWangJianhuaZhengXiaodong．StudiesontheextractingtechnicalconditionsofinulinfromJerusalemartichoke1528--200912tuberJ．JournalofFoodEngineering2007791087－1093．27．J．2008172297－300．28．J．200518213－14．29．J．2007282190－192．30SaleAJHHamiltonWA．Effectsofhighelectricfieldsonmicroor－ganismsJ．BiochimBiophysActa1967148781－788．31ZimmermannU．ElectricbreakdownelectropenneahilizationandelectrofusionRevJ．PhysBiochemPhamaeol1986105196－256．32Vego－MercadoHPothakamuryURChangFJetal．InactivationofEcolibycombiningpHionicstrengthandpulsedelectricfieldshurdlesJ．FoodResInt1996292117-121．33．J．2005269337－339．34．、J．200728253－55.35．J．2002276945－949.36．J．199710290－91.37．、J．2005365654－656．38．CPS2J．200627117－21．39．M．19981－2．40．J．200424244－46．41．J．200324427－30．42．J．2003311106－110．43．J．2007465821－823．44．J．－20003611490－492．45．、J．2007352445－48．46．J．20032411129－133．47．J．2008284115－117．48．J．1995224290-294．49ReinholdVNReinholdBBCostelloCE．CarbohydratemolecularweighprofilingsequencelinkageandbranchingdataEs-CIDJ．AnalChem19956711772-1784．50MockKKDaveyMCottrellJS．TheanalysisifunderivatizedoligosacchridesbymatrixssistedlaserdeaorptionmassspectrometryJ．BiochemBiophysResComm19911772644-651．ProgressofPolysaccharidesExtractionPurificationandIdentificationMethodsXuCuilian1DuLinru2FanSufang1SuHui1WanZhengkai11．CollegeofScienceHenanUniversityofAgricultureZhengzhou450002China2．CollegeofTobaccoHenanUniversityofAgricultureZhengzhou450002ChinaAbstractThispaperreviewstheextractionseparationandpurificationmethodsofanimalandplantpolysaccharidesandcomparestheadvantagesanddisadvantagesofeachmethod．Eachmethodhasitsownadvantagesanddisadvantagesappropriatemethodshouldbeselectedaccordingtothenatureofthechosenmaterialandsomeofthesemethodscanbesynergywithothermethodsundercertainconditions．Inadditionanalysisandidentificationofpolysaccharidesareoutlined．Keywordspolysaccharidesextractionseparationandpurificationanalysisandidentificationresearchprogress、1529--