2种四环素类抗生素对厌氧消化的最低抑制浓度和联合抑制类型

2013254120～123ActaAgriculturaeJiangxi2刘芳，颜智勇*，李刚，谭秀益，陈俊峰2012－12－06“”2008ZX07211－001201109024。1987─。*。410128厌氧消化技术被广泛用于猪场废水的处理，为探明四环素类抗生素对猪场废水对厌氧消化产生抑制作用的边界浓度值，选用了2种常用的四环素类抗生素土霉素和金霉素作为抑制剂，用重铬酸钾法测出COD，以COD去除率反映抗生素的独立抑制和联合抑制效果，结果显示:土霉素、金霉素对厌氧消化的最低抑制浓度分别是0．3、0．1mg/L，且2种抗生素的联合抑制效果大于单独抑制效果。土霉素;金霉素;单独抑制;联合抑制;COD去除率;边界浓度值X703．1A1001－8581(2013)04－0120－04LowestInhibitionConcentrationandCombinedInhibitingTypeofTwoTetracyclineAntibioticsagainstAnaerobicDigestionLIUFang，YANZhi－yong*，LIGang，TANXiu－yi，CHENJun－feng(CollegeofResourcesandEnvironment，HunanAgriculturalUniversity，Changsha410128，China)Abstract:Anaerobicdigestiontechnologyiswidelyusedinthetreatmentofpiggerywastewater．Inordertoinvestigatetheboundaryconcentrationofinhibitoryeffectoftetracyclineantibioticsagainstanaerobicdigestion，OxytetracyclineandChlortetracy-clinewerechosenastheinhibitors．PotassiumdichromatemethodwasusedtomeasureCODvalues，andCODremovalefficiencywasusedtoreflectthesingleinhibitionandcombinedinhibitionofthesetwoantibiotics．TheresultsshowedthatthelowestinhibitionconcentrationsofOxytetracyclineandChlortetracyclineagainstanaerobicdigestionwere0．3mg/Land0．1mg/Lrespectively，andtheircombinedinhibitingeffectwasstrongerthanthesingleinhibitingeffect．Keywords:Oxytetracycline;Chlortetracycline;Singleinhibition;Combinedinhibition;CODremovalefficiency;Boundaryconcentration0、。1980～200318%34%1。、、、30%～90%2。6000t330%～90%1800～5400t。mg/kgg/L10ng/L4－89－10。、。。2。11．11500mL35±1℃。3%NaOHCO2。11．22≥95%、、、CODCOD4000mg/L。MLVSS/MLSS0．5780～120m/h。470mL。35℃1d。1．31．3．1土霉素和金霉素单独对厌氧消化产生抑制的边界浓度值1070mL400mL。2541。1。35℃1、8、24、48、72hCOD11。2CODCOD。1/mg/L12340．30．50．70．90．10．30．50．71．3．2土霉素和金霉素联合对厌氧消化产生抑制的边界浓度值23。1．3．1。2/mg/L120．2990．30．0990．132/mg/L10．299×0．09920．299×0．130．3×0．09940．3×0．122．1OTC。20．3mg/L。80%0．3mg/LCOD。33h48hCOD。0．3mg/LCOD0．3mg/L0．3mg/L。2COD4。2．2CTC12142COD。30．1mg/LCODCOD。COD48h。40．1mg/L。4COD/mg/LR20y=16．781lnx+13．6870．9150．3y=18．268lnx+5．2530．9120．5y=18．474lnx+3．8160．9140．7y=18．62lnx+2．6130．9140．9y=19．065lnx－0．5380．9223COD5。5COD/mg/LR20y=17．583lnx+11．0430．9200．1y=17．696lnx+8．8210．9120．3y=18．318lnx+5．1820．9060．5y=18．900lnx+1．1830．9060．7y=19．55lnx－2．7590．893。0．299mg/L0．099mg/LCOD4。2COD0．299mg/L0．099mg/LCOD。4COD0．3mg/L0．1mg/L。6。62COD/mg/LR20y=15．598lnx+22．7440．9420．299y=15．351lnx+23．6260．9310．099y=15．465lnx+22．6840．9422．32241。22。2。22。3。2。4。2。2COD55COD。2。52COD3COD0．3mg/L、0．1mg/L。0．3mg/LCOD8%0．1mg/LCOD3%。。222125。2CODCOD2%。。1．J．20052271－274．2SarmahAKMeyerMTBoxallABA．AglobalperspectiveontheusesalesexposurepathwaysoccurrencefateandeffectsofveterinaryantibioticsVAsintheenvironmentJ．Chemosphere200665725－759．3ZhaoLDongYHWangH．ResiduesofveterinaryantibioticsinmanuresfromfeedlotlivestockineightprovincesofChinaJ．SciTotalEnviron20104081069－1075．4BoundJPVoulvoulisN．Pharmaceuticalsintheaquaticenvi-ronmentacomparisonofriskassessmentstrategiesJ．Chem-osphere200456111143－1155．5HuXGYiLZhouQXetal．Determinationofthirteenanti-bioticsresiduesinmanurebysolidphaseextractionandhighperformanceliquidchromatographyJ．ChineseJournalofAna-lyticalChemistry20083691162－1166．6BenWWQiangZMAdamsCetal．Simultaneousdetermi-nationofsulfonamidestetracyclinesandtiamulininswinewastewaterbysolid－phaseextractionandliquidchromatogra-phymassSpectrometryJ．JournalofChromatographyA20081202173－180．7VenglovskyJSasakovaNPlachaI．Pathogensandantibioticresiduesinanimalmanuresandhygienicandecologicalrisksre-latedtosubsequentlandapplicationJ．BioresourTechnol20091004386－5391．8MorleyNJ．EnvironmentalriskandtoxicologyofhumanandveterinarywastepharmaceuticalexposuretowildaquatichostparasiterelationshipsJ．EnvironToxicolPharmacol200927161－175．9．J．2009309136－141．10．J．20092981643－1648．11．D．2009．(责任编辑:曾小军櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗)(上接第119页)3．2。。1．J．200521135－37．2．M．1990330－380．3．J．200723281－88．4．J．2003123269－272．5．J．200931130－133．6．J．20102815830－15831．7．J．2004252116－120．8．J．2003123273－276．9．J．20103873685－3687．10．J．2010222139－141．11．J．200622528－33．12．J．2002113252－254．13．J．2011236129－131．(责任编辑:黄荣华)32142