A2O和SBR系统中微生物群落结构比较分析王亚超

476201712IndustrialMicrobiologyVol．47No．6Dec．2017doi10．3969/j．issn．1001－6678．2017．06．005513084402013JQ7018。1991～。[e-mail]。A2/OSBＲ1231231231231．7100002．7100003．71000016sＲNAA2/OA/O-SBＲ、。20、150。Proteobacteria、Bacteroidetes、Planctomycetes3380%。A2/ODechloromonas10．00%。SBＲThauera7．90%。CandidatusAccumulibacterSBＲ2．80%。Dechloromonas、ThaueraA2/OCandidatusAccumulibacter10．20%、3．70%0．50%。IlluminaMiSeqCandidatusAccumulibacter1。A2/OSBＲ、2。COD、、DO、SＲT。A2/OSBＲ。16sＲNAA2/OA/O-SBＲ、A2/OSBＲ、。11．1A2/OA/O-SBＲ。A2/O85%70%A/O-SBＲ98%。1－20℃。A2/OSBＲA2/OCOD350mg/LNH+4-NNH4Cl40mg/LPKH2PO410mg/LCaCl218mg/LMgSO420mg/L0．3mL/L。SBＲCOD350mg/LNH+4-NNH4Cl20mg/LPKH2PO410—52—mg/LCaCl218mg/LMgSO420mg/L0．3mL/L。1LEDTA10g、FeCl31．0g、CuSO4·5H2O0．03g、KI0．18g、CoCl2·6H2O0．15g、ZnSO4·7H2O0．12g、NH42Mo4O13·2H2O0．06g、MnSO4·7H2O0．12g、H3BO30．15g。1A2/OAAO1AAO2AAO3SBＲSBＲ1SBＲ2SBＲ3－20℃。1．216sＲNA16sＲNA。IlluminaMiSeqFASTQ。1FASTQ5bp1bp≥Q20≥99%Q20≥150bpNFLASHread1read2overlap≥10bp。IndexIndex。2OTUPCＲchimerasequence。5’＞1ambiguous＞8≤150bp。3OTU。。22．1PCＲchimerasequence。21195490%224nt～226nt。97%OTUOTU。6OTU22OTUChaoSimpsonShannonACEAAO1149814781374914397261848．320．987．521912．2230226AAO2158915671485959408271973．220．987．951998．5531351AAO31732170815741042428262069．990．998．202088．7540642SBＲ1152415031390874371201960．50．966．891920．6036841SBＲ2164916281501941388242109．480．987．512228．8736590SBＲ3160915911472921379232114．420．987．872198．1836304—62—647ChaoACE。SBＲA2/OOTU。SimpsonShannon、OTU1A2/OSBＲ。1OTU2．2A2/OSBＲ。A2/OSBＲ。15。21%Proteobacteria、Bacteroidetes、Planctomycetes、Chloroflexi、Chlorobi、Acidobacteria、Gemmatimonadetes、Verrucomicrobia8895%。Armatimonadetes、Chlamydiae、Firmicutes、Actinobacteria、Spirochaetes1%。A2/OSBＲ。Proteobacteria、Bacteroidetes、Planctomycetes3。2A2/OProteobacteria74．98%SBＲProteobacteria52．60%。3。Bacteroidetes、PlanctomycetesA2/O10．83%4．41%SBＲ25．00%8．50%。Chloroflexi、Chlorobi、Acidobacteria、Gemmatimonadetes、Verrucomicrobia、、。A2/O1．76%、0．06%、0．84%、0%0．73%SBＲ1．00%、1．00%、5．30%、2．10%3．50%。2、、375%～85%。、、、。、、3～5。3A2/OSBＲ。β-β-Proteobacteria、γ-γ-Proteobacteria—72—6A2/OSBＲ4745。A2/Oβ-γ-55．73%7．39%SBＲ19．70%28．30%CytophagiaSaprospirae、SphingobacteriiaA2/O2．14%、1．10%7．03%SBＲ9．00%7．00%、1．05%Planctomycetia、Phycisphaerae、OM190、C6A2/OSBＲ2．92%、1．19%、0．10%0．10%4．20%、2．00%、1．60%0．20%。Planctomycetia。2．3A2/OSBＲ15061%Dechloromonas、Planctomyces、Thauera、Aquicella、Dokdonella、Gemmata、CandidatusAccumulibacter、Acinetobacter、Polaromonas、Ｒubivivax、Azonexus。1%CandidatusMicrothrix、CandidatusProtochlamydia、Caldilinea、Kouleothrix、Gemmatimonas、Bradyrhizobium、Hyphomicrobium、Ｒhodobacter、Azovibrio、Bdellovibrio、Prosthecobacter、CandidatusXiphinematobacter。6A2/ODechloromonas10．20%SBＲ5．00%。SBＲ6—82—647Thauera7．90%A2/O3．70%。CandidatusAccumulibacter、Planctomyces、Gemmata、Dokdonella、Polaromonas、Ｒubivivax、Azonexus0．50%、1．20%、0．50%、3．10%、1．10%、2．10%1．02%2．80%、1．20%、1．20%、1．30%、0．10%、0．10%0．00%。A2/OSBＲ。2．43SBＲA2/OCandidatusAccumulibacter、、、、、、。CandidatusAccumulibacter、、、、、、、、。SBＲA2/OCandidatusAccumulibacter11。SBＲ2．80%A2/O0．50%SBＲA2/OSBＲ。DechloromonasA2/O10．20%SBＲ。PAO5。9。。SBＲA2/O。Thaueraspsp．strainTN910。SBＲA2/O11。3SBＲA2/O/%A2/OSBＲCandidatusAccumulibacter340．502．80Acinetobacter50．500．30Cytophagaceae60．106．80Planctomyces71．101．20Gemmatimonas80．100．20Dechloromonas910．205．00Thauera103．707．90Hyphomicrobium90．400．20。SBＲA2/OSBＲA2/O。A2/O、CandidatusAccumulibacterA2/OA2/OSBＲ。31SBＲA2/O。20、150。SBＲA2/O、、。A2/ODechloromonas10．20%。SBＲThauera7．90%。2CandidatusAccumulibacter、、、、、、7CandidatusAccumulibacterSBＲ—92—6A2/OSBＲ472．80%。Dechloromonas、ThaueraA2/O10．20%3．70%。CandidatusAccumulibacterA2/O0．50%。3。SBＲA2/OCandidatusAccumulibacterSBＲA2/O。A2/OA2/O。1．J．199929121-24．2．J．200838238-41．3．D．2014．4．EBPＲJ．201331139-42．5．J．201423321-26．6GEBＲEMAＲIAMSYBEUTELMWCHＲISTIANDetal．Ｒesearchadvancesandchallengesinthemicrobiologyofenhancedbiologicalphosphorusremoval-acriticalreviewJ．WaterEnvironmentＲesearch2011833195-219．7．16SrDNAMDAT-IATJ．2006266903-911．8ZHANGHSEKIGUCHIYHANADASetal．Gemmatimonasaurantiacagen．nov．sp．nov．agram-negativeaerobicpolyphosphate-accumulatingmicro-organismthefirstculturedrepresentativeofthenewbacterialphylumGemmatimonadetesphyl．nov．J．InternationalJournalofJystematicandEvolutionaryMicrobiology200353Pt41155-1163．9．16SrＲNAJ．201642-17．10．Thaueraspsp．strainTN9J．2013192318-323．11．D．2013．ComparativeanalysisofmicrobialpopulationstructureofA2/OandSBＲsystemWANGYachao123NANYaping123YUANLinjiang123CHENMin1231．InstituteofenvironmentalandmunicipalengineeringXi’anUniversityofArchitectureandTechnologyXian710000China2．KeyLaboratoryofNorthwestWaterＲesourcesEnvironmentandEcologyMinistryofEducationXian710000China3．KeyLaboratoryofEnvironmentalEngineeringXian710000ChinaAbstract16sＲNAhighthroughputsequencingmethodwasusedtocomparativeanalysisofmicrobialpopulationstructureofA2/OandSBＲsystem．20speciesatthephylumleveland150atthegenuslevelwereobtainedaccordingtothesequencingresults．MicrobialdiversityanalysisshowedthatthemainbacterialphylawereProteobacteriaBacteroidetesPlanctomycetesintheA2/OandSBＲsystemandthesumofthese3phylabacteriaaccountedforabout80%ofthemicroor