ABTS分光光度法测定水中微量二溴海因中的有效溴盛平

Vol.29,No.6,2010201029447-50WaterPurificationTechnology201029672-75WaterPurificationTechnology1,3--5,5-1,3-dibromo-5,5-dimethylhydantoinDBDMH、40[1]。、、。。[2]。[3]。。。HBrONH3＋1。25mg／L1mg／L[4]。ABTS2,2---3--6-、[5-6]。pH=2ABTSABTS1。ABTS＋1。ABTS12231.2000032.2004443.,200092DBDMH2,2'--3--6-ABTSpH=2ABTS·+ABTS405nm。0.08~1.61mg／L0.02mg／L。。2,2'--3--6-ABTSX830.2A1009-0177201006-0072-04DeterminationofAvailableBromineofTraceDibromohydantoinDBDMHinWaterbyABTSSpectrophotometryShengPing1,PuYunzhu2,HuangXin2,GaoNaiyun3(1.ChangzhengHospital,TheSecondMilitaryMedicalUniversity,Shanghai200003,China;2.SchoolofEnvironmentandChemicalEngineering,ShanghaiUniversity,Shanghai200444,China;3.StateKeyLaboratoryofPollutionControlandResourceReuse,TongjiUniversity,Shanghai200092,China)AbstractFordeterminationoflowlevelofdibromohydantoin(DBDMH)inwater,2,2'-azobis(3-ethylbenzothiazole-6-sulfonaicacid)(ABTS)spectrophotometricmethodwasusedtodetectavailablebromineinDBDMHunderwavelengthof405nm.ThemethodisbasedonthereactionofavailablebrominewithABTSwhenpHis2,whichformsastablegreenradical(ABTS·+).Agoodlinearre－lationshipwithavailablebromineonrangeof0.08~1.61mg／Lcanbeachieved,andthelimitofdetectionis0.02mg／L.TheeffectofammoniaproducedbythehydrolysisofDBDMHcanbeignored.Keywordsdibromohydantoin(DBDMH)2,2'-azobis(3-ethylbenzothiazole-6-sulfonaicacid)(ABTS)availablebrominewatertreatment[][]2008ZX07421-00250908138[]1980-。[e-mail]。72--DOI:10.15890/j.cnki.jsjs.2010.06.021WATERPURIFICATIONTECHNOLOGYVol.29,No.6,2010December25th,201011.1。Milli-Q。ABTS2,2-azinobis3-ethyl－ben-zothiazoline-6-sulfonicacid-diammoniumsalt。。ABTSHACH-DR2800。METROSEPASUPP5-250100μL3.2mmol／LNa2CO3／1.0mmol／LNaHCO30.7mL／min。5μg／L2μg／L。1.21.2.1HBrO、、HBrOAgNO3。Br2+AgNO3+H2O→AgBr↓+BrO-+NO3-+2H+1300nm、400nm、500nmHBrOεBr2300=9.0mol-1·L·cm-1、εBr2400=172mol-1·L·cm-1、εBr2500=37.3mol-1·L·cm-1εHBrO300=43.4mol-1·L·cm-1、εHBrO400=4.0mol-1·L·cm-1、εHBrO500=0.27mol-1·L·cm-1[7-8]。HBrOBr23%。HBrO10%HBrO。1.2.2pH2DBDMHKI。。2KI+Br2→2KBr+I22I2+2Na2S2O3→2NaI+Na2S4O6390.1504g、2gKI125mL。2mol／L20mL5minCmol／LV1mLV2mL1mol2mol1。TAB(totalavailablebromine,g／L)=(V1-V2)×10-3×C×0.5×79.941.2.3ABTS、、1mol＋12molABTS2mol405nm31600mol-1·L·cm-1。HBrO+2ABTS+H+→Br-+2ABTS·++H2O5NH2Br+2ABTS+2H+→Br-+2ABTS·++NH4+6NHBr2+4ABTS+3H+→2Br-+4ABTS·++NH4+7NH2Br+6ABTS+4H+→3Br-+6ABTS·++NH4+85HBrO10mLHBrO、1mL0.05mol／L1mL1g／LABTS25mL25mL1min405nm。ABTS。22.1ABTS0.08~1.61mg／LABTS2A＝1.243C－0.085R2＝0.998。7R20.991ABTSABTS·+Fig.1ABTSandOxygenRadical-O3SNH4+C2H5NNC2H5NH4+SO3-ABTS-O3SNH4+C2H5NNC2H5NH4+SO3-ABTS·+·NSSNN+SSN73--Vol.29,No.6,2010.ABTS2Fig.2CalibrationCurveofHBrOSD0.03k＝1.24±0.02L·mg·cm-1。0.16mg／L1.58mg／L52.75%1.34%1。MDL。73.1499%0.02mg／L。0.5mL25mL。0.5mL1.0mL2.0mL2。2.2DBDMH1040.2mg／L19.9%207mg／L。ABTS。DBDMH405nm0.010~0.018cm-10.008±0.002cm-1。。30.15~2mg／LABTS。DBDMH。ABTS3。15%。ABTS。ABTS4。t99%。5mg／L。ABTS0.24～2.11mg／L2.01.51.00.50.00.00.40.81.21.6／mg·L－1／cm-1R2＝0.9981Tab.1ResultsofParallelDetermination／cm-1／mg·L-1RSD／%0.1410.1320.1380.1380.1491.8751.8761.9281.9141.9280.1810.1740.1790.1790.1881.5761.5771.6191.6081.6190.181.602.751.342Tab.2ResultsofRecoveryExperiments／mg·L-1／mL／mg·L-1／mg·L-1／mg·L-1／%13.10.5120.260.521.050.260.550.761.3111.596.299n=3DBDMHC0=207mg／L+13ABTSDBDMHFig.3EffectofDilutionFactoronDeterminationofDBDMHbyABTSMethod1.81.61.41.21.00.80.60.40.20.00.00.20.40.60.81.01.21.41.61.8／mg·L－1ABTS／ng·L-12500125025035725012574--WATERPURIFICATIONTECHNOLOGYVol.29,No.6,2010December25th,2010。DBDMHDMH。CO2NH3。DBDMH。10mg／LDBDMH2mg／L120h0.2mg／L。HBrONH3。pH=2＋1ABTS1∶25、6、7、8。ABTSNH3。pH2DBDMH20μmol／LABTS·+5h3%。6。。3ABTS0.08~1.61mg／L0.01mg／L。ABTS。1,,,.[J].,2005,22(4):422-424.2,,,.[J].,2004,35(3):354-356.3,,.1--3--5,5-[J].,2001,18(11):807-810.4,,.[J].,2006,16(6):693,714.5UlrichPinkernell,BerndNowack,HerveaGallard,etal.Methodsforthephotometricdeterminationofreactivebromineandchlo－rinespecieswithABTS[J].WaterResearch,2000,34(18):4343-4350.6,,.ABTS[J].,2009,29(3):668-672.7NazafarinLahoutifard,PhilippeLagrange,JanineLagrange,etal.KineticsandMechanismofNitriteOxidationbyHBrO／BrO-inAtmosphericWaterandComparisonwithOxidationbyHClO／ClO-[J].PhysicalChemistryA,2002,106:11891-11896.8AtcharaSirimungkala,Horst-DieterFo1rsterling,VladimirDlask.BrominationReactionsImportantintheMechanismoftheBe－lousov-ZhabotinskySystem[J].PhysicalChemistryA,1999,103:1038-1043.9.[D].:,2008.3DBDMHTab.3ComparisonResultsofTwoMethodsforDBDHMDeterminationABTS／mg·L-1／mg·L-1／mg·L-112345669.19140.78206.46270.70405.09679.632500500250050025005002500500250050025005000.030.140.060.280.080.410.110.540.160.810.271.360.030.160.060.250.090.460.110.600.180.880.311.38ABTS4Tab.4AvailableBromineinDBDMHPills／%ABTS／%25.7724.3925.8025.9925.9024.7626.0725.2925.8024.77+2H2O→+2HBrO9CBrH3CCCOONBrCH3NCHH3CCCOONHCH3N→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→→75--