ANAMMOX与反硝化协同作用及氮素负荷研究朱明石

2　6环境工程学报Vol.2,No.620086ChineseJournalofEnvironmentalEngineeringJun.2008ANAMMOX朱明石　周少奇＊(,510006)　　630dUASB,,。COD(),,COD(),COD92.0%,23d,。,(NH+4-N)、(NO-2-N)(TN)0.063kg/(m3·d)0.063kg/(m3·d)0.126kg/(m3·d)0.239kg/(m3·d)、0.315kg/(m3·d)0.554kg/(m3·d),84.0%、93.0%85.0%,UASB。　　　　ANAMMOX　UASB　X703　　　A　　　1673-9108(2008)06-0737-06StudyofANAMMOX-denitrificationsynergisticinteractionandnitrogenloadingZhuMingshi　ZhouShaoqi(CollegeofEnvironmentalScienceandEngineering,SouthChinaUniversityofTechnology,Guangzhou510006)Abstract　TheUASB-biofilmreactorwasstartedsuccessfullyandstablyoperatedfor630days,andwhichwasaddedglucoseascarbonsource,theeffectivenessofANAMMOXprocessandnitrogenloadingratewerestud-ied.ContinuousincreasingorganicCOD(glucose)inANAMMOXreactorsystem,thereactoroperatedstably,andover92.0%ofCODremovalhadachieved.TheresultindicatedthatCODintheinfluenthadnoimportantinfluenceontheANAMMOXprocessinUASB-biofilmreactor.Itisfoundthatwithin23days,theANAMMOX-denitrificationsynergisticinteractionwasobtainedinthesamebio-reactorsystem.WhentheconcentrationofNH+4-N,NO-2-NandTNwereraisedfrom0.063kg/(m3·d),0.063kg/(m3·d)and0.126kg/(m3·d)to0.239kg/(m3·d),0.315kg/(m3·d)and0.554kg/(m3·d),theNH+4-N,NO-2-NandTNremovalrateswere84.0%,93.0%and85.0%,respectively.ItsuggestedthattheANAMMOXprocessinUASB-biofilmreac-torissuitedfortreatingbothlowandhighnitrogenconcentrationwastewater.Keywords　anaerobicammoniumoxidation;synergisticinteraction;denitrification;ANAMMOX;UASB:(20377013);(020959);:2007-10-29;:2008-01-17:(1982～),,,:。E-mail:[e-mail]＊,E-mail:[e-mail]　　(ANAMMOX),(NO-2-N),(NH+4-N)(N2)[1]。,、。,[2～17]。、[18～23]。,。,[23]。,2,。,。1　1.1　(UASB),3.2L,2.28L,0.92L[19]。PVC,。(32±1)℃,NaHCO3pH7.4～7.8。。[20],NH4Cl、NaNO2、MgSO4、KH2PO4、CaCl2、NaHCO3、Ⅰ(FeSO4)Ⅱ(H3BO3、ZnSO4·7H2O、CoCl2·6H2O、MnCl2·4H2O、CuSO4·5H2ONiCl2·6H2O)。。1.2　。(NH+4-N):[24];(NO-2-N):N-(1-)-[24];(NO-3-N):[24]。pH:PHS-3C。(COD):XJ-1COD[24]。2　　　[1],,。[18,23],,。ANAMMOX:18NH+4+1-2fs2+3fs109NO-2+20fs109CO2+　　3-4fs4+3fs109H+=fs5.45CH2O0.5N0.15+　　1-fs4N←+fs8NO-3+5-8fs8+36fs109H2O(1):fs。(1),NH+4、NO-2ANAMMOXCO2,NO-3,H+,pH。:5C6H12O6+24NO-3=12N2←+30CO2+18H2O+24OH-(2)C6H12O6+8NO-2=4N2←+6CO2+2H2O+8OH-(3),CODCO2,ANA-MMOX,。。2,。1∶1.32,、0.168kg/(m3·d)0.223kg/(m3·d),。(),。COD1。COD330～380mg/L。1,COD3:、。:COD87%,53%,COD。COD,COD,COD,;,COD,COD。1～2d,COD。:,(3～13d)COD,50%～60%,,。,,,COD,COD75%,,COD7386:ANAMMOX,3d89%,,。:COD90.0%,COD25～35mg/L。,23d,。2,,,。1∶1.32,0.168kg/(m3·d)、0.223kg/(m3·d)(2),()3d,32.5mg/L55.6mg/L,86.5%76.8%;,30mg/L,90%～91%(3)。,(2、3),(4～14d),43mg/L,82.1%;,90.5%。COD,60%。,,15d36.2mg/L23d14.6mg/L,93.9%;,15d90.6%23d91.2%。15～23d9d,COD,89.6%。,,、COD95%、91%90.5%,COD30mg/L。23d,,、COD,、99%,90%,COD93.5%。3　NH+4-N、NO-2-NCODFig.3　VariationofNH+4-N,NO-2-NandCODremovalefficiency,UASB-。0.39kg/(m3·d)、COD0.26kg/(m3·d),ANAMMOX,(23d)。3　630d,NH+4-NNO-2-N99.9%,NH+4-NNO-2-N,TN90%。(NH+4-N)、(NO-2-N)0.063kg/(m3·d),NH+4-N、NO-2-N99.9%。(4),pH7.3～7.8,(0.1～0.2kgTN/(m3·d))1∶1,7392(0.2～0.4kgTN/(m3·d))、(0.4～0.6kgTN/(m3·d))1∶1.32,50mg/L(),、0.239kg/(m3·d)、0.315kg/(m3·d)0.554kg/(m3·d)。5～10d。30d(4)、0.098kg/(m3·d),,、92%、98%,(NO-3-N)40mg/L。、,60d、0.204kg/(m3·d)、0.264kg/(m3·d),0.468kg/(m3·d),,、90%、97%,(5)85%,,35mg/L,。65d,、1∶1.32,290mg/L382.8mg/L,0.204kg/(m3·d)0.269kg/(m3·d),,15～36mg/L。,、0.239kg/(m3·d)0.315kg/(m3·d),、84%93%,、46mg/L30mg/L,,84%,,40mg/L,。,,,340mg/L448.8mg/L,788.8mg/L,0.239kgNH+4-N/(m3·d)、0.315kgNO-2-N/(m3·d)0.554kgTN/(m3·d)。,,,85%,,ANAMMOX,。、,。,UASB-,、。,pH,pH,;,。NH+4,NO-2ANAMMOXCO2,H+,pH[23]。,、pH(5),pH;(、),、pH,pH,pH,110d,,。Strous[7],:NH+4+1.32NO-2+0.066HCO-3+0.13H+※1.02N2+0.26NO-3+0.066CH2O0.05N0.15+2.03H2O(4)7406:ANAMMOX、1∶1.32∶0.26,,0.26。,(6),,、-。5NH+4+3NO-3※4N2+2H++9H2O(5)G'0=-297kJ/mol,。1～50d,,,(5),。6　NO-3-NFig.6　VariationofNO-3-Nduringoperation3,1,,50mgNO-3/(L·h);21,16mgNO-3/(L·h),1,;3,,,5.4mgNO-3/(L·h)。,,。[18,23],CO2,,;NO-3。-,NO-3,,。4　　(1)(),,23d,。240mg/L316.8mg/L,COD330～380mg/L,COD90.0%,COD25～35mg/L。0.39kg/(m3·d)、COD0.26kg/(m3·d),ANAMMOX,(23d)。(2)630d,NH+4-NNO-2-N99.9%,NH+4-N、NO-2-N。NH+4-N、NO-2-N,NH+4-N、NO-2-N,、NH+4-NNO-2-N,85%～92%、97%～99%,85%。NH+4-N、NO-2-NTN0.239kg/(m3·d)、0.315kg/(m3·d)0.554kg/(m3·d),,,。UASB-,、。(3)、。,,CO2,,;NO-3。,。。[1]MulderA.,vandeGraafA.A.,RobertsonL.A.,etal.Anaerobicammoniumoxidationdiscoveredinadenitrifyingfluidizedbedreactor.FEMSMicrobiol.Ecol.,1995,16:177～1847412[2]StrousM.,FuerstJ.A.,KramerE.H.M.,etal.Missinglitotrophidentifiedasnewplanctomycete.Nature,1999a,400:446～449[3]JettenM.S.M.,StrousM.,vandePas-SchoonenK.T.,etal.Theanaerobicoxidationofammonium.FEMSMicro-biol.Rev.,1999,22:421～437[4]Mosquera-CorralA.,GonzalezF.,etal.Partialnitrifica-tioninaSHARONreactorinthepresenceofsaltsandor-ganiccarboncompounds.ProcessBiochemistry,2005,40:3109～3118[5]StrousM.,etal.Keyphysiologyofanaerobicammoniumoxidation.Appl.Environ.Microbiol.,1999,65(7):3248～3250[6]EgliK.,FangerU.,AlvarezP.J.J.,etal.Enrichmentandcharacterizationofananammoxbacteriumfromarota-tingbiologicalcontactortreatingammonium-richleachate.ArchivesofMicrobiology,2001,175(3):198～207[7]StrousM.,HeijnenJ.J.,KuenenJ.G.,etal.These-quencingbatchreactorasapo