BurkholderiaVie省略miensis对水中结晶紫的降解程迎

、、、[1-3]。[4]。、、。[5]。、、、。。[6]、[7][8]。。,16SrRNA。11.11.1.1C09V。1.1.2LBg·L-151010g·L-1KH2PO41.8Na2HPO4·12H2O3.5FeCl3·6H2O0.01MgSO40.10.032%。1.1.3722NSPX-2508-ZSKY-2102BurkholderiaVietnamiensis350007116SrRNABurkholderiaVietnamiensisC09V。C09VKNO3pH5.05%35℃36h30mg·L-196.5%。BurkholderiaVietnamiensisX703.1A1000-3770(2011)10-0046-0042011-06-09UE0021)[e-mail]3710201110TECHNOLOGYOFWATERTREATMENTVol.37No.10Oct.,201146DOI:10.16796/j.cnki.1000-3770.2011.10.013XSP-8CAPHS-3CpH。1.21.2.110mL10mg·L-130℃、150r·min-1。0.1mL150mg·L-1。1mL1070.1mL30℃2d。、、4～5-40℃。1.2.2。。PCR95℃5min94℃1min55℃1min72℃1min30s3072℃10min30℃3min。16SrRNAC09V16SrRNANCEGenbankBlastBurkholderiavietnamiensis100%BurkholderiavietnamiensisC09VNCEGenBankJF922108。1.2.320mL150r·min-120h50mL7000r·min-110min3。1.2.4100mL250mL30℃、150r·min-130mL7000r·min-110min722。%=A0-AeA0×100%1A0Ae。600nmOD600。22.1C09V2%、3%、4%、5%、6%、7%30℃、150r·min-154h1。2%～5%5%97.2%。1。[9]。2.2C09V。C09V5%、、、pHpH30℃150r·min-142hKNO3、NH4Cl、(NH4)2SO4、、pHpH30℃150r·23456780828486889092949698100
/%/%1Fig.1EffectofinoculumsonbiodegradationofcrystalvioletBurkholderiaVietnamiensis47min-142h。97.8%86.7%78.9%58.3%25.9%。C09V。。Re-activeRed141[10]Flavodonflavus[11]Phanerochaetechrysosporium[12]。KNO396.1%63.8%(NH4)2SO461.1%46.4%42.2%。C09VKNO396.1%。C09VC09V。2.3pHC09VC09V5%、KNO3pHpH20、25、30、35、40℃150r·min-142h2。2C09V20～35℃25～35℃C09V75%35℃97.9%40℃。[13]。[14]40℃C09V20℃。C09V5%、KNO330℃150r·min-1pH4.0、5.0、6.0、7.0、8.042h。3pH4.05.0pH5.08.0pHC09V96.4%75.2%。C09V＞75%pH。2.4C09V5%、KNO330℃150r·min-1pH5.06h4。30h30h93.1%42h98.8%。46h6h30h15202530354045020406080100
/%2Fig.2Effectoftemperatureonbiodegradationofcrystalviolet3pHFig.3EffectofinitialpHonbiodegradationofcrystalviolet3456789020406080100
/%pH37104836h。2.55C09V30mg·L-1-。5589nm300nm350nm12h。2。589nmC09V。。。3C09V35℃pH5.05%KNO3。54h97.2%42h98.8%。pH。[1],.[J].,2005,30(3):30-33.[2]KirbyN,MarchantR,McmullanG.DecolourisationofsynthetictextiledyesbyPhlebiatremellosa[J].FEMSMicrobiologyLetters,2000,188:93-96.[3],,,.[J].,2002,22(1):57-60.[4],,.[J].,2009,36(7):1046-1051.[5]AzmiW,SaniRK,BanerjerUC.Biodegradationoftriphenylmethanedyes[J].EnzymeMicrobTechnol.,1998,22:185-191.[6]EichlerovaI,HomolkaL,NerudF.Syntheticdyedecolorizationcapacityofwhiterotfungusdichomitussqualens[J].BioresourceTechnol.,2006,97:2153-2159.[7]YatomeC,YamadaS,OgawaT,etal.Degradationofcrystalvioletbynocardiacorullina[J].ApplMicrobioiBiotechnol.,1993,38:565-569.[8]DaneshvarN,AyazlooM,KhataeeAR,etal.Biologicaldecoloriza-tionofdyesolutioncontainingmalachitegreenbymicroalgaeCos-mariumsp[J].BioresourceTechnol.,2007,98:1176-1182.[9]MellefontL,McMeekinT,RossT.Effectofrelativeinoculumconcentrationonlisteriamonocytogenesgrowthinco-culture[J].InternationalJournalofFoodMicrobiology,2008,121:157-168.[10]TelkeA,KalyaniD,JadhavJ,etal.Kineticsandmechanismofreactivered141degradationbyabacterialisolaterhizobiumradiobacterMTCC8161[J].ActaChimSlov.,2008,55:320-329.[11]RaghukumarC,RivonkarG.Decolorizationofmolassesspentwashbythewhite-rotfungusflavodonflavus,isolatedfromamarinehabitat[J].ApplMicrobiolBiotechnol.,2001(55):510-514.[12]PantD,SinghA,SatyawaliY,etal.Effectofcarbonandnitrogensourceamendmentonsyntheticdyesdecolourizingefficiencyofwhite-rotfungus,phanerochaetechrysosporium[J].JournalofEnvironmentalBiology,2008,29:79-84.[13]LopezZavalaM,FunamizuN,TakakuwaT.Temperatureeffectonaerobicbiodegradationoffecesusingsawdustasamatrix[J].WaterResearch,2004,38:2406-2416.[14]BamforthS,SingletonI.Bioremediationofpolycyclicaromatichydrocarbons:currentknowledgeandfuturedirections[J].JournalofChemicalTechnology&Biotechnology,2005,80:723-736.0510152025303540450.00.51.01.52.02.53.0
/%/hOD0204060801004Fig.4Growthanddegradationcurveunderoptimumcondition5-Fig.5UV-visspectraofcrystalvioletsolution3004005006007008000.00.51.01.52.02.53.03.5
/nm59BurkholderiaVietnamiensis49THEBIODEGRADATIONOFCRYSTALVIOLETBYBURKHOLDERIAVIETNAMIENSISChengYing,GanLi,ChenZuliang（SchoolofChemistryandMaterialsScience,FujianNormalUniversity,Fuzhou350007,China）Abstract:Acrystalviolet-degradingbacterium,designedstrainC09Vwasisolatedfromtheactivatedsludgeoftheeffluenttreatmentsystem(aprintinganddyeingmill,fujian,china).ItwasasubspeciesofBurkholderiaVietnamiensisbasedonGramstaining,morphologycharacters,andbiochemicaltests,aswellastheanalysisof16SrRNAgenesimilarities.Batchexperimentswereperformedundervariousconditions,andthedatashowedthat96.5%ofcrystalvioletcouldbedegradedinthesolutioncontaining30mg·L-1crystalviolettestedattheoptimawithin36h:includingglucose,KNO3,5%inoculumssize,pHat5.0,andthetemperatureof35℃.Keywords：burkholderiavietnamiensis;crystalviolet;biodegradation;decolorizationCATALYTICREDUCTIONOFNITRATEINWATEROVERPd-Cu/ACCATALYSTWangKunping1,2,LiuMiaomiao1,XuQianqian1,GuoJinsong1,2,WangChunyan1,ZhaoYue1（1.KeyLaboratoryEco-EnvironmentsofThreeGorgesReservoirRegionundertheStateMinistryofEducation,ChongqingUniversity,Chongqing400045,China;2.CollegeofCityConstructionandEnvironmentEngineering,ChongqingUniv