您好,欢迎访问三七文档
当前位置:首页 > 商业/管理/HR > 质量控制/管理 > DF活体浮游植物在线监测结果与叶绿素a的关系研究
DFa1221.3328002.3300021。,,,,,。,,。,、、a,[1]。a,[2,3]。a,,。a[4],,[5]。(DelayedFluorescence,DF),,,[6]。1951,[7],,,。、,。,。DF。、、、,,[8]。、、(),[9-12],。2011,,DF,。DF6,a,a,DFYSI6600a.6aa.aaS946TV213.3A1004-4701201301-0011-06DOI:10.3969/j.issn.1004-4701.2013.01.04391Vol.39No.120133JIANGXIHYDRAULICSCIENCE&TECHNOLOGYMar.20132013-01-16“948”201109.1985-.。22.1,。,。(Microcysisaeruginosa)、(Anabeana)、(Scenedesmusquadricanda)、(OvalCryptophyta)、(Cyclotella)、、,。DF、(、)、(、、)4。2.2DFa,YSI6600a,,,,a。2.2.1EXCELDF6a,y=ax。2.2.2a(、)、、()(),DF,YSI6600a。6a,a(y=ax1+bx2…fx6)。2.2.3a,/a,,。,,,a(a')y=a'(ax1+bx2…fx6),a。2.3DF、,,,a,,,a(a″)y=a″a'(ax1+bx2…fx6),a。33.1a1DF,a,/a,,a。1,,a。1a。,y1,y2,y3,y4,y5、、、a,x1,x2,x3,x4,x5x6SB、B、TG、YG、RSR。,(1),a(2)。212201331aa,。,1a,,a(y)(x)。a,::y1=0.00067x1+0.0005x2+0.00033x3+0.00017x4+0.00017x5+0.00017x6:y2=0.0031x1+0.71×0.0022x2+0.0029x3+1.54×0.0047x4+0.0032x5+0.66×0.0021x6:y4=0.0042x1+0.74×0.0031x2+0.82×0.0035x3+1.26×0.0052x4+1.21×0.005x5+0.0038x6:y5=0.0027x1+0.62×0.0018x2+0.67×0.0020x3+1.68×0.0040x4+1.17×0.0030x5+0.55×0.0016x61aa/(μg·L-1)SBBTGYGRSR14340262941497092901303029356328.01/333856295346522703138751983.01/916232924171411171506334338.02.2aDF,3。a。a(y)(x),。1/a,,4。,。,a,a,5。5,a,。,a,25。、、、4.116、0.5955、1.4336、0.7206,,3aaa14.4119.9138.5172.4445.2150.051/27.764.274.392.5238.779.501/44.336.241.852.0134.340.05μg/L2aRSBy3=0.023x10.99970By3=0.0125x20.99980TGy3=0.022x30.99950YGy3=0.0354x40.99990Ry3=0.0252x50.99985SRy3=0.0112x61.00000y3=0.0038x1+0.54×0.0021x2+0.0037x3+1.57×0.0059x4+1.10×0.0042x5+0.48×0.0019x6391DFa13、、a::y1=4.116×(0.00067x1+0.0005x2+0.00033x3+0.00017x4+0.00017x5+0.00017x6):y2=0.5955×(0.0031x1+0.71×0.0022x2+0.0029x3+1.54×0.0047x4+0.0032x5+0.66×0.0021x6):y3=1.4336×(0.0038x1+0.54×0.0021x2+0.0037x3+1.57×0.0059x4+1.10×0.0042x5+0.48×0.0019x6):y4=0.7206×(0.0042x1+0.74×0.0031x2+0.82×0.0035x3+1.26×0.0052x4+1.21×0.005x5+0.0038x6)14201335a0.62.5336.9822.5953.7879.124.8218.351/20.321.2719.8111.328.8239.5513.329.181/40.180.6311.155.6516.2319.787.484.59μg/L44.1730.8538.8214.4011.76%/(ug·L-1)/(ug·L-1)3.02.52.01.51.00.50.00.00.20.40.60.8y=-4.116xR2=0.98952a/(ug·L-1)/(ug·L-1)25201510500.010.020.030.040.0y=-0.5955xR2=0.98953a/(ug·L-1)/(ug·L-1)90807060504030201000204060y=-1.4336xR2=0.98944a/(ug·L-1)/(ug·L-1)201816141210864200102030y=-0.7206xR2=0.98945a4,20128d,、、a,,a6。,,a。,,TSSDF。,,aa,3。30.0386,,a::y1=0.0386×4.116×(0.00067x1+0.0005x2+0.00033x3+0.00017x4+0.00017x5+0.00017x6):y2=0.0386×0.5955×(0.0031x1+0.71×0.0022x2+0.0029x3+1.54×0.0047x4+0.0032x5+0.66×0.0021x6):y3=0.0386×1.4336×(0.0038x1+0.54×0.0021x2+0.0037x3+1.57×0.0059x4+1.10×0.0042x5+0.48×0.0019x6):y4=0.0386×0.7206×(0.0042x1+0.74×0.0031x2+0.82×0.0035x3+1.26×0.0052x4+1.21×0.005x5+0.0038x6)a6,a,4。,,a1,R20.7787,,。6aYSI6600aYSI6600a0.0386SBBTGYGRSR184484086180782882659.732.732.69278275375175574578054.642.192.11350247751146649052935.232.232.13444343944146546044832.600.931.45542441240741441441829.861.461.58686814621082780854138969.122.143.05798616471140844950151075.884.223.4781086183812688731004171582.273.523.33μg/L3a4aa/(ug·L-1)a/(ug·L-1)Y=0.0386xR2=0.7787Y=0.9998xR2=0.77870501000.01.02.03.04.0543210543210a/(ug·L-1)a/(ug·L-1)391DFa154DF6a。,DF、。a。,DFa。,,。。[1],,,.a[J].,2009,25(6):40-43.[2](),,().[M].:,1985:901-904.[3],.[M].:,1990:268-270.[4].a[J].,2005(1):55-57.[5],,.[J].,2004,28(11):1-2,5.[6]IstvánovicsV.,HontiM.,OsztoicsA.,H.M.Shafik,PadisákJ.,Y.YacobiandW.EckertOn-linedelayedfluorescenceexcitationspec-troscopy,asatoolforcontinuousmonitoringofphytoplanktondynamicsanditsapplicationinshallowLakeBalaton(Hungary)[J].FreshwaterBiology,2005,50:1950-1970.[7]StrehlerB,ArnoldW.J.Gen.Physiol.,1951,34:809[8],.[J].,2006,15(3):236-239.[9]HontiM.,IstvánovicsV.andOsztoicsA.Measuringandmodellinginsitudynamicphotosynthesisofvariousphytoplanktongroups[J].Verh.Internat.Verein.Limnol.2005,29:194-196.[10]HontiM.,IstvánovicsV.andOsztoicsA.Stabilityandchangeofphy-toplanktoncommunitiesinahighlydynamicenvironmentthecaseoflarge,shallowLakeBalaton(Hungary)[J].Hydrobiologia,2007,581:225-240.[11]HontiM.,IstvánovicsV.andKozmaZs.AssessingphytoplanktongrowthinRiverTisza(Hungary)[J].Verh.Internat.Verein.Limnol,2008,30(1):87-89.[12]IstvánovicsV.andHontiM.LongitudinalvariabilityinphytoplanktonandbasicenvironmentaldriversalongTiszaRiver,Hungary[J].Verh.Internat.Verein.Limnol,2008,30(1):105-108.1620133StudyonrelationshipbetweenthemonitoringresultsofOn-lineDelayedFluorescenceexcitationspectroscopyandchlorophyll-aLIUAi-ling1LI-Mei2XIONGLi-li21.HydrologybureauofPoyangLakeJiangxiXingzi332800China2.HydrologybureauofJiangxiProvinceNanchang330002ChinaAbstract:Thealgaecontentinwateristhekeytodeterminetheeutrophication.InthispaperweculturedseveralrepresentativespeciesofalgaeofthePoyangLakethendeterminedthebiomassbytheOn-lineDelayedFluorescenceexcitationspectroscopyDFandtestedthechlorophyll-awiththeMulti-parameterwaterqualityanalyzerYSI6600.Byanalyzingtherelationshipbe-tweenthesixfluorescencevaluesandchlorophyllacontentthecalibrationformulasbetweenasinglespeciesofalgaeandmixedalgaechlorophyllaandfluorescencevalueswereestablished.T
本文标题:DF活体浮游植物在线监测结果与叶绿素a的关系研究
链接地址:https://www.777doc.com/doc-6535823 .html