DNPAOS的富集及其同步除磷脱氮的实验研究唐艳葵

25　320079　　　　:JournalofGuangxiNormalUniversity:NaturalScienceEdition　　　　　　Vol.25　No.3Sep.2007:2007-01-29:(0448085);(X051008):(1965-),,,;(1963-),,,,,。DNPAOS,,,(,530004)　:,//,(DNPAOS),。,,,58/,93%97%,DNPAOSPAOS48%。:;;;:X506　　　:A　　　:1001-6600(2007)03-0117-05。、,[1]。(PAOS)“,”,、,。:(PAOS),,,(DNPAOS),ATP,、β(PHB);,,(),[2]。,NH+4NNO-2N,O2[3]。2080,Pseudomonasflurescens、Alcaligenesfacealis、Pseudomonasaeruginos。,、,,[4,5]。NH+4NNO-2NNO-3N,DNPAOS。DNPAOS,,[6],。,,DNPAOS,,。1　1.1　、:、、、、,。:WMXCOD、-(U3210,Hitachi,Tokyo,Japan)、WM-ZA、GS12-2、、。DOI:10.16088/j.issn.1001-6600.2007.03.0221.2　;;COD-,。。1.3　。(5L),。3g/L。/,,4mg/L,PAOS。COD150～210mg/L([7])、15.0～20.0mg/L20.0～25.0mg/L,,3h、3h。15d/,DNPAOSPAOS。,COD()180～220mg/L、15.0～22.0mg/L20.0～25.0mg/L(20min),,6h;CODTP25.0～30.0mg/L、20.0～25.0mg/L、10mg/L(20min),1mg/L,6h。,[8],DNPAOS。2　2.1　15/,TP32%63%,TPPAOS。1/(TP)。,2～14,1　/NH+4NTPFig.1　NH+4NandTPremovalafteranoxicperiodintheanaerobic/anoxicprocessTP40%,,PAOS,。30,,,TP70%。40,DNPAOS,TP97.0%,(80%)。1～40,,4293%。,DNPAOS。2.2　/COD、/COD,,42、48、55,0min,2a～d。,3。,TP200min,,,TP10mg/L26mg/L(2a);2b,COD200min,。,COD,COD,,(AC-)PAOS(polyhydroxyalkanoates,PHA),PHB(poly-3-hydroxybutyrate)PHV(poly-3-hydroxyvalerate)[9],ATPADP,ATP/ADP,,ADPATP,,,,[10]。118　　　　　　　　　　　　　　　　:　　　　　　　　　　　　　　252　/COD、Fig.2　TimecourseofCODNH+4NandTPinanaerobic-anoxicperiod3　Fig.3TimecourseofNO-3Nintheanoxicperiod4　Fig.4TimecourseofNO-2Nintheanoxicperiod　　2c,,50min,,COD,,COD,。,,NO-3,,,ATP/ADP,,,。2d,,DNPAOS100min,,DNPAOsPHB,,PHBNO-3NNO-2N。PHB,,PHB。3.78mg/(L·h)。DNPAOs,NO-3NO-2,NO-3NO-2。,NO-3NO-2[11]。,4855(45)(34)。3,NO-3N100min,,NO-3N,,NO-3N,NO-3N0.05mg/L。4,15min,,0.09mg/L,NO-3NNO-2N,1193　　　　　　　　　　　:DNPAOS　　　　　　　　　　DNPAOS,DNPAOS。3、4:15min,,。:,,,,。NO-3NO-2[12]。,+,,DNPAOSPHB(-β-)。2.3　DNPAOSPAOS,PAOS2:A,B,DNPAOSPAOS[13,14]:DNPAOS/PAOS=/。/58,/,DNPAOS/PAOS=0.48,DNPAOSPAOS48%,DNPAOS/PAOS=0.19,DNPAOS,。3　:(1)DNPAOS,//,DNPAOS,。(2),,,,NO-3NO-2DNPAOS。NO-3NO-2。(3)+,DNPAOSPHB。　　　:[1]　,,.[J].:,2004,22(2):81-87.[2]　BORTONEG,SALTARELLIR,ALONSOV,etal.Biologicalanoxicphosphorusremoval——thedephanoxpro-cess[J].WaterScienceandTechnology,1996,34(1-2):119-128.[3]　KERRNJESPERSENJP,MOGENSH.Biologicalphosphorusuptakeunderanoxicandaerobicconditions[J].Wa-terResearch,1993,27(4):617-624.[4]　ELISABETHV,MUNCHPL,JURGK.Simultaneousnitrificationanddenitrificationinbench-scalesequencingbatchreactor[J].WaterResearch,1996,30(2):277-284.[5]　COLLIVIGNARELLIC,BERTANZAG.Simultaneousnitrification/denitrificationprocessinactivatedsludgeplant:performanceandapplicability[J].WaterScienceandTechnology,1999,40(4/5):187-194.[6]　,.[J].,2003,19(9):32-34.[7]　LEMOSPC,VIANAC,SALGUEIROEN,etal.Effectofcarbonsourceontheformationofpolyhydrox-yalkanoates(PHA)byaphosphate-accumulatingmixedculture[J].EnzymeandMicrobialTechnology,1998,22(8):662-671.[8]　CHENYin-guang,CHENYin-sheng,XUQing,etal.Comparisonbetweenacclimatedandunacclimatedbiomassaf-fectinganaerobic-aerobictransformationsinthebiologicalremovalofphosphorus[J].ProcessBiochemistry,2005,120　　　　　　　　　　　　　　　　:　　　　　　　　　　　　　　2540(2):723-732.[9]　MEINHOLDJ,CARLOSDMF,GLENTD,etal.Characterizationofthedenitrifyingfractionofphosphateaccu-mulatingorganismsinbiologicalphosphateremoval[J].WaterScienceandTechnology,1999,39(1):31-42.[10]　,.[J].,2003,19(8):33-35.[11]　.-COD[J].,1997,23(6):12-15.[12]　LIUYan,ZHANGTong,HERBERTHPF.Microbialcommunityanalysisandperformanceofaphosphate-re-movingactivatedsludge[J].BioresourceTechnology,2005,96(11):1205-1214.[13]　WACHTMEISTERA,KUBAT,vanLOOSDRECHTMCM,HEIJNENJJ.Asludgecharacterizationassayforaerobicanddenitrifyingphosphorusremovingsludge[J].WaterResearch,1997,31(3):471-478.[14]　SATOSHIT,TAKASHIO,KOICHIS,etal.Simultaneousnitrogenandphosphorusremovalusingdenitrifyingphosphate-accumulatingorganismsinasequencingbatchreactor[J].BiochemicalEngineeringJournal,2006,27(3):191-196.EnrichmentofDNPAOSandItsSimultaneousDenitrificationandPhosphateRemovalTANGYan-kui,TONGZhang-fa,ZHANGHan-bing,WANGXiao-ying(SchoolofChemistryandChemicalEngineering,GuangxiUniversity,Nanning530004,China)Abstract:Theselectionandenrichmentofheterogenousnitrifyingbacteria(HNBs)anddenitrifyingphos-phateaccumulatingorganisms(DNPAOS)werecultivatedinthesameEnhancedPhosphateRemovalRe-actor(EPER)inalaboratoryscale.Thereactorwasrunfirstunderanaerobic/aerobicandthenanaero-bic/anoxiccondition.Afterthe58treatmentcycles,theproportionofDNPAOSincreasedanddomi-naled.TheNH+4Neliminationofmorethan93%andphosphateremovalof97%wereobservedintheanoxicperiodandtherateofDNPAOStoPAOSwas48%.Keywords:enhancedphosphateremovalreactor;denitrifyingphosphate-accumulatingorganisms;deni-trification;phosphateremoval(责任编辑　马殷华)1213　　　　　　　　　　　:DNPAOS