EGSB反应器中厌氧颗粒污泥的脱氮特性研究康晶

　,.EGSB[J].,2005,25(2):208-213KANGJing,WANGJianlong.ThecharacteristicsofanaerobicammoniumoxidationbygranularsludgefromEGSBreactor[J].ActaScientiaeCircumstantiae,2005,25(2):208-213EGSB康　晶,王建龙＊,　100084:,(EGSB),3,,,.EGSB,,EGSB,.,TN、45.5%、13.4%99.9%;TN、54.3%、21.7%99.9%.NH+4-N,3.17mg·L-1·h-1(N).,EGSB.:;;;;;EGSB;.:0253-2468(2005)02-0208-06　　:X703　　:AThecharacteristicsofanaerobicammoniumoxidationbygranularsludgefromEGSBreactorKANGJing,WANGJianlongLaboratoryofEnvironmentalTechnology,INET,TsinghuaUniversity,Beijing100084Abstract:Anexpandedgranularsludgebed(EGSB)reactorwasstarted-upbyseedingtheanaerobicsludgefrombrewerywastewatertreatmentplant.Afterthreemonthsoperation,thegranularsludgeoccurredinthereactor,andtheammoniumnitrogencouldberemovedcontinuously.Themorphologyandinnerstructureofthegranuleswasobservedusingscanningelectronmicroscopy(SEM)andthephotographsindicatedthattheanaerobicgranularsludgewasrelativelyregularinshape.Theouterlayerhadaratherfluffyandloosestructureandwasmoretransparentcomparedtothecenterofthegranule.Thecenterofthegranulehaddensestructure.Thelong-termoperationalresultsshowedthatthemaximalremovalefficiencyofNH+4-Nwas40%wheninfluentNH+4-Nconcentrationwas70～250mg·L-1;theNO-2-Nremovalefficiencywasmorethan98%wheninfluentNO-2-Nconcentrationwas70～250mg·L-1;andtheCODremovalefficiencywas84%attheinfluentconcentrationof500mg·L-1.Thisrevealedthatthereactionofanaerobicammoniumoxidation(ANAMMOX)probablytakenplaceintheEGSBreactor.InordertovalidatetheexistenceofANAMMOXreactionandinvestigatetheperformanceofnitrogenremovalbyanaerobicgranularsludge,thegran-ularsludgetakenfromEGSBreactorandtheactivatedsludgetakenfrompartial-nitrificationreactorwasusedtotreatthesyntheticwastewater.Theexperimentalre-sultsdemonstratedthatfortheactivatedsludge,theremovalefficiencyoftotalnitrogen(TN),NH+4-NandNO-2-Nwas45.5%,13.4%and99.9%,respective-ly;fortheanaerobicgranularsludge,theywere54.3%,21.7%and99.9%,respectively.TheremovalrateofNH+4-Nwasstable,about3.17mg·(L·h)-1.Atthebeginningofthereaction,theANAMMOXprocesswasperformedmainlythroughthepathwayofinteractionbetweenNH+4-NandNO-2-N,astimewenton,withtheexhaustionofNO-2-N,thegranularsludgecoulduseNO-3-NaselectronacceptortooxidizeNH+4-N,yieldingdinitrogengas.Inthisresearch,theCODremovalefficiencywasabout90.4%.InthelightofestimationofCODconsumption,theanaerobicgranularsludgecoulduseorganiccarbonsourceaselectrondo-nor,reduceNO-2-NandNO-3-N,tocarryoutthetraditionaldenitrification.Theresultsobtainedinthisstudycouldrevealthatintheanaerobicgranularsludgere-actortheANAMMOXreactiontakenplace,accordingly,theexistenceofanaerobicammoniumoxidationphenomenonintheEGSBreactorcouldbededuced.Keywords:ANAMMOX;nitrogenremoval;wastewatertreatment;nitrification;denitrification;EGSB;granularsludge:2004-08-19;:2004-08-30:(:59978020):　(1979—),,;＊,[e-mail]　　,.,,-、、[1,2].(ANaerobicAMMoniumOXidation,ANAMMOX),2090DelftKluyver[3].,25220052　　　　　ACTASCIENTIAECIRCUMSTANTIAEVol.25,No.2Feb.,2005DOI:10.13671/j.hjkxxb.2005.02.014.,[3],:(1),N2O,N2O;(2),,NO;NON2O,N2ON2;(3)NH+4,、N2H2N2.Mulder,,[4]:5NH+4+3NO-34N2+9H2O+2H+G′0=-297kJ·mol-1(1)　　,[5].vandeGraaf,[6]:NH+4+NO-2N2+2H2OG′0=-358kJ·mol-1(2)　　1,1NO-2,1NH+4.(NH2OH)(N2H4)ANAMMOX,NH2OH,NH2OHHNO2,1.1　Fig.1　ThepathwayofNtransferduringanammoxprocess[7～21].,,、pH、[7].2,[22],.EGSB,,2,、、COD,,EGSB.1　1.1　EGSB,2.37℃,..EGSB70mm,600mm,2.3L;140mm,160mm,2.46L;4.6L.2　EGSB(1.;2.;3.;4.;5.;6.;7.;8.;9.;10.;11.;12.)Fig.2　EGSBreactorconfigurationforanaerobicammoniumoxidation1.2　,(NH4)2CO3、NaNO2、CaCl2、MgSO4、Ⅰ(EDTA、FeSO4)、Ⅱ(EDTA、ZnSO4·7H2O、CoCl2·6H2O、MnCl2·4H2O、CuSO4·5H2O、Na2MoO4·2H2O、NiCl2·6H2O、Na2SeO4·10H2O、H3BO3).,HRT1.20d.1.3　2500mL,EGSB,250mL,500mLEGSB2092　:EGSB,100mg·L-1,COD650mg·L-1.,37℃,.N22～3min,,.,.2min,18G(12mm)10～15mL,8～10h.1.4　[23].:;:N-(1-)-;:;COD:.2　　　,(EGSB),3,EGSB,　　.,(SEM),3SEM.24.,2NO-2-N5.,,,NO-2-N.2COD6.NO-3-N7.,NO-3-N12.4mg·L-12.6mg·L-1,99.1%24.3%.3　　　3,,(3A),.,.,、(3B、3C),　　3　SEMFig.3　ScanningelectronmicrographsofanaerobicgranularsludgecultivatedinEGSBreactor210　　　　　　　　　　254　NH+4-NFig.4　TimecourseofNH+4-Nremoval6　CODFig.6　TimecourseofCODremoval5　NO-2-NFig.5　TimecourseofNO-2-Nremoval7　NO-3-NFig.7　TimecourseofNO-3-Nremoval(3D).,NO-2-N70～250mg·L-1,98%;COD500mg·L-1,84%;NH+4-N70～250mg·L-1,40%,2～30mg·L-1(N).EGSB.4,3d,NH+4-N21.7%,NH+4-N13.3%.NH+4-N.2h,NO-2-N,NH+4-N;NO-2-N,NH+4-N.NO-2-N,NH+4-N.EGSB,NH+4-NNO-2-N,NO-2-N,NO-2-N.,.,NO-2-N.,、,;,,.45:NO-2-N,,.,30.6mg·L-1NO-2-N,57.8mg·L-1NO-2-N.2h,NH+4-NNO-2-N,.NH+4-NNO-2-N.NH+4-NNO-2-N.,.6,20h,COD,,,COD,2112　:EGSB.,.COD91.6%,COD81.6%.COD.46,,NH+4-NCOD,NH+4-N.,NH+4-N,COD.,.NH+4-NCOD,.,COD,,COD.4、5,,NH+4-N10mg·L-14.5mg·L-1,NO-2-N16mg·L-10.7mg·L-1.5,10h,NO-2-N.4,10h,NH+4-N.VandeGraaf[6],NH+4NO-31∶0.6,NH+4NO-21∶1.Strous,,5mmol·L-1NH+4NO-3[9]:NH+4+1.32NO-2+0.066HCO-3+0.13H+1.02N2+0.26NO-3+0.066CH2O0.05N0.15+2.03H2O(3)　　、,.,.7,.,(1).,NH+4-NNO2-N、NO-3-N1∶2.63∶0.41.NH+4-NNO-2-N1∶2.63,Strous(3),COD,.(3),,.4～7,TN52.8mg,45.5%.9.4mg,13.4%.43.6mg,99.9%.vandeGraaf[10],,,,,.、、、,,.4～7,TN65.7mg,54.3%,NH+4-N152.8mg,21.7%,NO-2-N44.2mg,99.98%.