Fe2SO43对活性污泥微生物活性的影响

44620126JOURNALOFHARBININSTITUTEOFTECHNOLOGYVol.44No.6Jun．2012Fe2SO431211LudovicoSpinosa311．1500902．1500013．74100Fe2SO4320、40、60、80100mg·L－1Fe2SO434h、RSOU、EPSCOD．Fe2SO4320～60mg·L－1、RSOU、EPSCOD、TP、UV254Fe2SO43．Fe2SO4380mg·L－1、RSOU、EPS．Fe2SO43100mg·L－1、RSOUEPS．COD、TP、UV254SCODNH3－N．Fe2SO43RSOUEPSX503.2A0367－6234201206－0001－05TheeffectofFe2SO43onmicrobialactivityofactivatedsludgeTANGJin-hua12XUGuo-ren1XIAOJing1LudovicoSpinosa3LIGui-bai11．StateKeyLaboratoryofUrbanWaterResourceandEnvironmentHarbinInstituteofTechnology150090HarbinChina2．Dept．ofFoodandEnvironmentalEngineeringHeilongjiangEastUniversity150001HarbinChina3．Dept．ofEnvironmentalEngineeringandSustainableDevelopmentTechnicalUniversityofBari74100TarantoItalyAbstractTostudytheeffectofFe2SO43asflocculantonmicrobialactivityofactivatedsludgeFe2SO43indosagesof20406080100mg·L－1wasdroppedintoASSandfourhourslatterDHARSOUandEPScontentwereexaminedaswellasCODetal．TheresultsshowedthatDHARSOUandEPScontentwereinsensitivetoFe2SO43inrangeof20－60mg·L－1andtheremovalratesofCODTPandUV254ineffluentincreasedgreatlywiththeconcentrationofFe2SO43increased．WhentheconcentrationofFe2SO43was80mg·L－1DHARSOUandEPScontentdecreaseddistinctly．WhentheconcentrationofFe2SO43was100mg·L－1DHAandRSOUwereinhibi-tedfurtherwhereastotalEPScontentincreasedgreatly．MeanwhiletheremovalratesofCODTPandUV254inef-fluentincreasedslowlyandtheremovalratesofSCODandNH3-Ntendedtodecline．KeywordsFe2SO43DHAspecificoxygenuptakerateRSOUextracellularpolymericsubstancesEPS2011－09－10．2009AA06470451038003NCET-08-161．1981—1968—LudovicoSpinosa1944—．1931—[e-mail]2SO43、．Fe3+．1．Fe3+Fe3+．TTC－、RSOUFe3+Fe2SO43EPS．Fe2SO43．11.1．1d1．1UV254ρCODmg·L－1ρSCODmg·L－1ρTPmg·L－1ρNH3-Nmg·L－1pH0.3～0.45300～550130～2705～850～707．0～7.5Fe3+Fe2SO43Fe3+．1.2TTC-2－3Dt=De+Ds．Dt0.5mL15mLTris-HCl3mL、0.36%Na2SO31mL0.4%TTC2mL．37±1℃30min37%1mL．5kr/min5min80%5mL37±1℃10min5kr/min5min．485nm．105±1℃1h．De0.5mL15mL10mL4r/min5min5min3．Tris-HCl3mL、0.36%Na2SO31mL0.4%TTC2mLDt．DsDs=Dt－De．1.3ROURSOU350mL5minDO．300mL．DODO0．1mg·L－1．ExcelkROURSOU=k·60/X．RSOUMLSSmg·g·h－1XMLSSg·L－1kmg·L·min－1．1.4EPS1.4.1EPS1H2SO48%H2SO450mL300r/min1h．7．3kr/min10min0.45μm．2-NaOH0.15mL30min0.04%50mL300r/min1h．7．3kr/min10min0.45μm．30.9%NaCl50mL1min．7．3kr/min10min0.45μm．40.9%NaCl50mL80℃30min7．3kr/min10min0.45μm．5NaOH0.9%NaCl50mL1mol·L－1NaOHpH1110min7．3kr/min10min0.45μm．650mL300r/min1h．7．3kr/min10min0.45μm．1.4.2EPS4Lowry5DNA6·2·44260nmDNAmg·L－1ρDNA=D260nm/0.020．1.520406080100mg·L－1Fe2SO4354hFe2SO43．2g·L－1．4h30minCOD、SCOD、TP、UV254、RSOU、EPS．22.1Fe2SO43Fe2SO431．Fe2SO43COD、SCOD、NH3-N、TPUV254Fe2SO43COD、TPUV254Fe2SO4360mg·L－1COD、TPUV25474%、92%38%Fe2SO4330%、80%14%Fe2SO43COD、TPUV254SCODNH3-NFe2SO43SCODNH3-NFe2SO43Fe2SO43SCODNH3-N．10080604020%204060801001200籽(Fe2(SO4)3)/(mg·L-1)去除率/%CODSCODNH3鄄NTPUV2541Fe2(SO4)3COD、SCOD、NH3鄄N、TPUV2542.2Fe2SO43TTC-Fe2SO43TTC－2．Fe2SO4320～60mg·L－1DtFe2SO4360～100mg·L－1Dt134.791.8μg·mg·h－1Fe2SO43．Fe2SO43Fe2SO43DeFe2SO4320～60mg·L－160mg·L－1De．Fe2SO43100mg·L－1De48.2μg·mg·h－198.4μg·mg·h－150%．DsFe2SO43Fe2SO4380mg·L－1Ds58.5μg·mg·h－115.4μg·mg·h－1Fe2SO43DsDeDt．16012080400%20406080100120DtDeDs%%%%籽(Fe2(SO4)3)/(mg·L-1)脱氢酶活性/(滋g·（mg·h)-1）2Fe2(SO4)3TTC鄄Fe3+、7．Fe3+．Carter8．．2.3Fe2SO43RSOU9．10ROURSOU．Fe2SO43RSOU3Fe2SO4320～60mg·L－1RSOUFe2SO43Fe2SO4360mg·L－1MLSSRSOU1.5mg·g·h－145.9mg·g·h－1．·3·6Fe2SO43Fe2SO4360mg·L－1100mg·L－1RSOU．Fe2SO43100mg·L－1MLSSRSOU30.3mg·g·h－1．2、3RSOUDtFe2SO43Fe2SO43Fe2SO43．．ⅠⅡ．RSOU———DtTTCO2．11．TTC－RSOU．020406080100120%%%%籽(Fe2(SO4)3)/(mg·L-1)RSOU／（mg·(g·h)-1）3Fe2(SO4)3RSOU5046423834301Fe2SO4360mg·L－1COD、TP、UV254SCODNH3-N．Fe2SO4360mg·L－1Fe2SO43．2.4Fe2SO43EPS2.4.1EPS2EPS．VSSEPS8.5mg·g－1DNA0.7mg·g－1．EPSDNAEPS．DNA．．5EPS-NaOHNaOHH2SO4．DNADNA、EPS．EPS5DNAVSS28.6mg·g－1．-NaOHNaOHEPSNaOHpHEPSEPSEPSEPS12．-NaOHNaOHEPSDNANaOHNaOH．-NaOH30min1hNaOHpH1110min-NaOHEPSNaOH．2EPSmg·g－1H2SO4-NaOHNaOHDNA13.215.94.828.611.20.726.415.15.832.116.93.130.499.614.671.492.44.7EPS70．0130.625.2132.1120.58.52.4.2Fe2SO43EPSFe2SO43EPS4．1401201008060402020406080100120%%%%籽(Fe2(SO4)3)/(mg·L-1)%%%%%%w/(mg·g-1)DNA多糖蛋白质EPS04Fe2SO43EPS4Fe2SO43EPS·4·4420～60mg·L－1Fe2SO43Fe2SO4380mg·L－1EPSFe2SO43100mg·L－1EPS8.3%4.6%．Fe2SO4320～80mg·L－1Fe2SO43100mg·L－1VSS10.1mg·g－118.5mg·g－183.2%．Fe2SO43DNA．4Fe2SO4320～80mg·L－1EPSFe2SO43DtRSOU20～60mg·L－1Fe2SO43EPSDtRSOU80mg·L－1Fe2SO43．Fe2SO43．Fe2SO43100mg·L－1Fe2SO43Fe2SO43DtRSOUEPS．EPS13．Fe2SO43100mg·L－1EPSEPS．31TTC－RSOUFe2SO43DtRSOUFe2SO43．2Fe2SO4360mg·L－1COD、TP、UV254SCODNH3-NFe2SO43．．35EPSNaOHDNA、．4Fe2SO4320～80mg·L－1EPSFe2SO43DtRSOU．Fe2SO43100mg·L－1Fe2SO43DtRSOUEPS．1．DHAETSJ．201028697－99．2．J．200218950－52．3WANGFFDINGYHGELetal．Effectofhigh-strengthammonianitrogenacclimationonsludgeactivityinsequencingbatchreactorJ．JEnvironSci201022111683－1688．4FROLUNDBPALMGRENRKEIDINGKetal．Ex-tractionofextracellularpolymersfromactivatedsludgeusingacationexchangersinJ．WaterRes19963081749－1758．5RAUNKJARKHVITEDJTNIELSENPH．MeasurementofpoolsofproteincarbohydrateandlipidindomesticwastewaterJ．WaterRes1994282251－261．6．M．1998．7RASMUSSENHNIELSENPH．IronreductioninASmeasuredwithdifferentextractionte