Fe2和Fe3对厌氧氨氧化污泥活性的影响李祥

3511201411ENVIＲONMENTALSCIENCEVol．35No．11Nov．2014Fe2+Fe3+1212*1212121.2150092．215009Fe．05mg·L－15mg·L－1．．71Fe2+Ｒ10.28kg·m3·d－10.65kg·m3·d－1Fe3+Ｒ21.28．Fe2+．Fe3+Ｒ21.17Fe2+Ｒ11.24．X703.1A0250-3301201411-4224-06DOI10．13227/j．hjkx．2014．11．0262014-04-042014-05-2951408387201104XKY2011031984～[e-mail]*[e-mail]2+andFe3+ontheActivityofANAMMOXLIXiang12HUANGYong12WUChuan12WANGMeng-ke12YUANYi121．SchoolofEnvironmentalScienceandEngineering．SuzhouUniversityofScienceandTechnologySuzhou215009China2．InstituteofEnvironmentalBiotechnologySuzhouUniversityofScienceandTechnologySuzhou215009ChinaAbstractTheeffectofthechangeinconcentrationandvalenceofironionontheANAMMOXsludgeactivitywasstudiedbyinoculatingANAMMOXsludge．TheconcentrationexperimentalresultsshowedthattheANAMMOXsludgeactivitywasstimulatedandgraduallyincreasedwhentheinfluentironionconcentrationincreasedfrom0to5mg·L－1．IronhydroxideprecipitatewasformedwiththepHvalueincreasedduetothealkalinityoftheANAMMOXproductwhentheinfluentironionconcentrationwashigherthan5mg·L－1whilethebiologicalactivitywasnotinhibited．TherewasnosignificantdifferenceintheeffectsoftheconcentrationchangeofironionswithdifferentvalenceeffectsontheANAMMOXsludgeactivity．Thelong-termexperimentalresultsshowedthatthenitrogenremovalrateasNofanammoxreactorＲ1wasincreasedfrom0.28kg·m3·d－1to0.65kg·m3·d－1withFe2+ininfluentwhichwas1.28timesashighasthatintheANAMMOXreactorＲ2containingFe3+ininfluentafter71cyclesofcultivation．ThereforeFe2+couldbettermeetthegrowthneedsofANAMMOXbacteria．TheresultsalsoshowedthatFe3+easilyledtotheexcessiveconversionofammoniaintheANAMMOXreactor．Theconversionratiobetweennitrateandammoniawas1.17inreactorＲ2whichwassignificantlylowerthanthatinthereactorＲ1withaconversionratiobetweennitrateandammoniaof1.24.KeywordsANAMMOXironionvalencereactionprocesseffectNH+4-NNO－2-N．．1．2～4．、pH、、5～8．、9．daGraaff10Ca2+．．vanNiftrik11Fe．12Strous13Fe．FeFe11Fe2+Fe3+．Fe．Fe2+Fe3+FeFe3+．Fe．Fe．11.1100mL500mL．5cm．32℃pH6.5～6.60.1mol·L－1pHFe．1.2200814．NH+4-N、NO－2-N98%20kg·m3·d－1．0.5～2mmMLVSS/MLSS0.7～0.8．1.3．NH4Cl、NaNO2、NaHCO31000mg·L－1、KHCO31000mg·L－1、KH2PO427mg·L－1、CaCl2·2H2O136mg·L－1、MgSO4·7H2O200mg·L－1、Ⅰ1mL·L－1Ⅱ1.25mL·L－1．ⅠEDTA5000mg·L－1ZnSO4·7H2O430mg·L－1CoCl2·6H2O240mg·L－1MnCl2·4H2O990mg·L－1CuSO4·5H2O250mg·L－1NaMoO4·2H2O220mg·L－1NiCl2·6H2O190mg·L－1NaSeO4·10H2O210mg·L－1H3BO414mg·L－1ⅡEDTA5000mg·L－1FeSO4FeCl3Fe2+Fe．30min．1.4《》15．NH+4-NNO－2-NN-1-NO－3-NpHpH21119Fe2+、Fe3+．1.5Fe2+、Fe3+Fe242g．100mL8h5%1.251.331.21.2812Fe．Fe2．Fe2+Fe3+94.2mg·L－1、128mg·L－110hFe2+、Fe3+．Fe2500mL10gＲ1、Ｒ2．Ｒ1Fe2+Ｒ2Fe3+．10g10h．Fe．22.1Fe2+Fe3+Fe2+Fe3+1．1aFe2+．Fe2+0mg·L－15mg·L－150.47mg·L－166.37mg·L－117.57mg·L－129.51mg·L－1．．16FeSO40.1mol·L－1Fe2+5.6mg·L－1．12Fe5224350.075mmol·L－14.2mg·L－1Fe0.03mmol·L－11.68mg·L－1Fe．Fe2+5mg·L－165mg·L－117.99mg·L－130.86mg·L－1Fe．1Fe2+Fe3+Fig．1EffectofthechangeinFe2+andFe3+concentrationonnitrogenremovalefficiencyofANAMMOXsludgeFe3+Fe2+1b．Fe3+05mg·L－150.4mg·L－166.6mg·L－116.66mg·L－129.56mg·L－1Fe3+．Fe3+5mg·L－165mg·L－116.46mg·L－133.88mg·L－1Fe．3、9．Fe．FeFeFe．Fe5mg·L－1Fe5～6mg·L－1．FeFeOH3FeOH2FeOH32.64×10－39．OH－pH．Fe．Fe2+Fe3+．2.2Fe2+Fe2+Fe2+5mg·L－1Fe2+Fe2+5.05～5.08mg·L－1．2Ｒ1100.2mg·L－1128mg·L－146.1mg·L－154.4mg·L－10.28kg·m3·d－1．622411Fe2+Fe3+Ｒ1．Ｒ13598%0.47kg·m3·d－1．Ｒ136120mg·L－1150mg·L－12.6mg·L－17mg·L－10.56kg·m3·d－1．Ｒ160140mg·L－1175mg·L－10.76kg·m3·d－1．126.58mg·L－17.5mg·L－129.55mg·L－10.65kg·m3·d－1．2Fe2+Fig．2Long-timeeffectofFe2+onnitrogenremovalefficiencyofANAMMOX2.3Fe3+Fe3+Fe3+5mg·L－1．3100.2mg·L－1128mg·L－146.75mg·L－166.6mg·L－10.29kg·m3·d－1．57Ｒ295.2mg·L－1122mg·L－17.2mg·L－116.5mg·L－10.43kg·m3·d－1．Ｒ258120mg·L－1150mg·L－10.65kg·m3·d－1．Ｒ27112.9mg·L－123.5mg·L－10.51kg·m3·d－1Ｒ10.78．Fe3+Fe2+．3Fe3+Fig．3Long-termeffectofFe3+onnitrogenremovalefficiencyofANAMMOX33.1FeStours131.320.2617．Fe2+Fe3+4．Fe2+Ｒ11.240.22Strous18．1920．Fe3+Ｒ21.170.19．Fe3+Ｒ2Fe2+Ｒ1．12Fe2+1．02～1．15．．Fe2+．Fe2+Fe3+Fe3+．Shrestha21722435NH+4Fe3+N2．Ｒ2．Ｒ2Fe3+．4Fe2+Fe3+Fig．4EffectofFe2+andFe3+onnitrogenconversionratioofANAMMOX3.2FeFe．．Zhu22Ｒhodobactersphaeroides3．2mg·L－1．Wei23Fe3+Fe3+Fe3+1.6～3.3C．．．2682425．Fe．Zhang26Fe2+．Fe2+．Fe2+Fe3+．5mg·L－1．Strous181.8mg·L－1．pHFe．Fe．Fe2+Fe3+FeFe3+．FeFe3+．Fe2+Fe3+．Fe3+Fe2+．41Fe2+Fe3+Fe．Fe05mg·L－1．Fe5mg·L－1OH－FeFe．2Fe71Fe2+0.28kg·m3·d－10.65kg·m3·d－1、1.240.22Fe3+0.29kg·m3·d－10.51kg·m3·d－11.170.19．Fe2+．1．J．20133392359-2368．2．J．20132761078-1083．822411Fe2+Fe3+3LiHSZhouSQMaWHetal．Faststart-upofANAMMOXreactorOperationalstrategyandsomecharacteristicsasindicatorsofreactorperformanceJ．Desalination2011286436-441．4LvYTWangLWangXDetal．MacroscaleandmicroscaleanalysisofANAMMOXinanaerobicrotatingbiologicalcontactorJ．JournalofEnvironmentalSciences201123101679-1683．5LottiTvanderStarWＲLKleerebezemＲetal．TheeffectofnitriteinhibitionontheANAMMOXprocessJ．WaterＲesearch20124682559-2569．6ChenJWZhengPTangCJetal．EffectoflowpHontheperformanceofhigh-loadedANAMMOXreactorJ．JournalofChemicalEngineeringofChineseUniversities2010242320-324．7JungJYKangSHChungYCetal．Factorsaffectingtheactivityofanammoxbacteriaduringstartupinthecontinuousculturereactor