您好,欢迎访问三七文档
当前位置:首页 > 行业资料 > 其它行业文档 > pH对间歇进水序批式生物反应S省略性污泥沉降性能和微生物结构的影响璩绍雷
35320163ENVIRONMENTALCHEMISTRYVol.35No.3March20162015731ReceivedJuly3120115.*07JCZDJC02100.SupportedbyAppliedBasicResearchProjectofTianjin07JCZDJC02100.**Tel13132002241E-mailBaosheng_sun@sina.comCorrespondingauthorTel13132002241E-mailBaosheng_sun@sina.comDOI10.7524/j.issn.0254-6108.2016.03.2015073101.pHSBRJ.2016353508-515QUShaoleiSUNBaoshengZHAOShuanghongetal.pHactivatedsludgesedimentationperformanceandthestructureofthemicrobeofSBRtechnologyJ.EnvironmentalChemistry2016353508-515pHSBR***300072pHSBRpH5.0—10.0.SVI.pH5.06.0.pHDNAPCRShannon.pH.pHSBR.pHactivatedsludgesedimentationperformanceandthestructureofthemicrobeofSBRtechnologyQUShaoleiSUNBaosheng**ZHAOShuanghongZANGXiangrongLIZhijingWEIJiahongSchoolofEnvironmentalScienceandEngineeringTianjinUniversityTianjin300072ChinaAbstractThispapermainlystudiedtheinfluenceofpHonSBRsludgebulkingandmicrobialstructurewiththepHvaluesoftheinflowwaterfrom5.0to10.0.TheresultsshowedthatthedidnotchangeinitiallyofSludgeVolumeIndexSVIvalue.HoweverthesludgebulkingwasseenovertimewhenpHvaluesbecame5.0and6.0.AtvariouspHvaluesthetotalbacterialDNAgroupswereextractedforPCRamplificationandthenrelevantanalysesweregivenaboutShannondiversityindexclonedsequencingandphylogenetictreeofpartialdominanttotalbacteriainthemicrobialcommunities.ThisstudydemonstratedthatthestructuresandquantitiesofmicroorganismsweredifferentatdifferentpHvaluesinacidicconditionsfilamentousbacteriabecamedominantandtheyweremoreaccessibletosludgebulkinginneutralandalkalineconditionwhilezoogloeawerethedominantonesandtheactivityofsludgewasrelativelystable.KeywordspHSBRtechnologyactivatedsludgemicrobialstructure..1.FilamentousbulkingNon-filamentous3pHSBR509bulking.90%.pH6.0—8.0pH6.5..pHpH5.0—10.0.pHSBRpHSBRpHSBRpH-PCR-DGGEpH.1Materialandmethods1.1SBR14L6∶42—4mg·L-1.22℃—24℃...1Fig.1Textreactionequipment1.224hCOD、、MLSSSVISVI90mL·g-1COD、、85%.SBRpH5.0—10.0.4000mL·g-1pHpH..12hC6H12O6、NH42SO4、KH2PO3BOD5∶N∶P=100∶5∶1MgSO4、CaCl2、FeCl3.Cl-Na+2.0.5mol·L-1HClNaOHpH5.0、6.0、7.0、8.0、9.0、10.0.1COD650—51035820mg·L-17.5—8.2mg·L-140.97—49.46mg·L-145—54.5mg·L-1.1Table1ThecomponentsandconcentrationsofartificialdistributionwaterComponentC6H12O6NH42SO4KH2PO3MgSO4·7H2OCaCl2·2H2OFeCl3·6H2OConcentration/mg·L-180022036501048h4h12hpH.COD、MLSS、SV、SVI.20dpH20dpH.pH.1.3DNA1.3.1pH30mL50mL3.1.3.2DNA-//-DNA5mL4mL37℃30min10%SDS55℃1h9165r·min-110min///20.13mol·L-1-20℃2h6—10℃、9165r·min-110min70%100TEDNAA260/A280A260/A230DNA0.7%70V30min.4.1.4PCRDNAPCR16SrDNAV3F357-GC5'-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCCCTACGGGAGGCAGCAG-3'“GC”5R5185'-ATTACCGCGGCTGCTGG-3'.240bp.50μL10—100ng25μL2×TaqPCRMasterMix1μL25μmol·L-1、50μL.PCR6.1.5PCRDGGEC.B.S.SCIENTIFICDGGE-2001PCR7.1.6Shannon-WeaverQuantityOneDGGEShannonSBR.HH=-∑si=1PilgPi=-∑si=1ni/N()lgni/N()Pi=ni/NniN.2Resultsanddiscussion2.1Rob83pHSBR511、3.SVIpH.SVI70—100mL·g-1.pHEPS9.pHpH.2SBRSVIpH5.06.070mL·g-120dSVI170mL·g-1、.pHpH7.0—10.0SVI67—85mL·g-1pH=10.0SVIpH5.06.0.、、pH5.06.0.pH.pHpH.2Fig.2ThechangeofsludgeconcentrationandsludgevolumeindexpHpH5.06.0pH=7.0pH8.0—10.0pH5.0、6.0.EPS9.2.2DGGEpH20dpHpHpHpHpH.pH=10.0C1pH=9.0C2pH=8.0C3pH=7.0C4pH=6.0C5pH=5.0C6.pHPCRDGGE3pHpH.512353pHDGGEFig.3DGGEprofilesoftotalbacterialunderdifferentpHpHDGGE32.2pHDGGETable2DGGEprofilesbandclassificationoftotalbacterialunderdifferentpHGraphfeatureTypicalstripepHBand156910pHBand171819pHBand234pHBand111214pHBand7131516pHBand83pH.pHBand6Band17、18、19pHBand2、3、4pHBand12pHpHBand16α-Band8.2.333Uncu-lturedbacterium.Band4Band18Rhodanobactersp..Band3、12、16、17、19ProteobacteriaProteoba-cteria.Band3、3pHSBR51312、19γ-Gammaproteobac-teriumBand16α-alphaprote-obacteriumα-FISHα-10—1213—15pH=5.0.Band6Chloroflexi16.1718pH=6.0Band6pH.16SrDNA97%94%.5691%90%Unculturedbacteriumclone11-43UnculturedChlor-oflexibacteriumisolateDGGEgelBand4.DNAMANGenbank4.316SrDNATable316SrDNASequencinganalysisofpartofthemicrobialcommunityofthedominantbacteriaBandlength/bpGenbankGenbankcomparethesequenceaccessionnumbersimilarspecieshomologyBand2123GQ8711UnculturedbacteriumcloneUTFS-R12-214-10699%Band3123JX51225Unculturedgammaproteobacteriumisolate98%DGGEgelbandPHAs-29Band4149KF4474Rhodanobactersp.7B-39399%Band5154GU5606Unculturedbacteriumclone11-4390%Band6132HM1640UnculturedChloroflexibacteriumisolateDGGEgelBand491%Band8162EU6383UnculturedZoogloeasp.cloneSHBZ77599%Band9161HQ6579UnculturedRhodocyclaceaebacteriumclonehrb-11899%Band10116GQ8791UnculturedbacteriumcloneUTFS-R12-214-9597%Band12125HQ8268UnculturedgammaproteobacteriumcloneN1199%Band13133CU9148UnculturedTM7bacterium97%Band14150JN67916UnculturedXanthomonassp.clone5.17m24100%Band16125GQ3540UnculturedalphaproteobacteriumcloneMS11-2098%Band17122EU2916UnculturedproteobacteriumcloneGASP-KC2S2_C0698%Band18149KF7365UnculturedRhodanobactersp.clone11.17_7_898%Band19122EU2806UnculturedgammaproteobacteriumcloneAS13798%2.4Shannon-WienerpH.C1—C6Shannon-WienerC1=0.942C2=0.943C3=1.06C4=0.946C5=0.928.pHpH8.0Shannon1.06pH7.0、9.0、10.0ShannonpH=6.0pH=5.0pH7.0、9.0、10.03pH.3Conclusion1pH.pH..514354DNABLASTFig.4Phylogenetictreeoftotalbacteriasystemaccordingtoth
本文标题:pH对间歇进水序批式生物反应S省略性污泥沉降性能和微生物结构的影响璩绍雷
链接地址:https://www.777doc.com/doc-6539966 .html