pH值对剩余污泥微氧水解酸化溶出物及微生物群落结构的影响曾薇

1005-0930201803-0471-012X703Adoi10.16058/j．issn．1005-0930.2018.03.0022017-05-132017-12-30515780162016YFC04011031974—[e-mail]1211111．“”1001242．225200．pH．VFAsVolatilefattyacids、COD、pH10.0TVFATotalvolatilefattyacid4156.4mgCOD/LTVFA52%3～4．pH4.0Proteobacteria59.9%38.6%pH10.0Firmicutes70.9%FirmicutesClostridia63.4%．．pH、．．C/N12-3．、．．Chen461%、11%、1%27%．5．26320186JOUＲNALOFBASICSCIENCEANDENGINEEＲINGVol．26No．3June2018VolatilefatacidsVFAs6-8．0.2～1.0mg/LVFAs9．VFAs、、．VFAs10-11VFAs．VFAs．pHVFAs12-13．pHVFAs．pH．11．11．91L1Fig．1Ｒeactoroftest900mL．35℃100r/min．1～8pH4.0、5.0、6.0、7.0、8.0、9.0、10.011.04mol/LNaOH2mol/LHCl9pH0.2～0.5mg/L．pH12h48h．1．2A2/O4℃24h．1．1Table1CharacteristicsofwasteactivatedsludgeWASpH6．88TSS18680VSS12765CODSCOD45．15NH+4-N5．63SOP7．27COD23．25COD5．5pHmg/L．274Vol．261．30.45μmVFAs、CODSCOD、NH+4-N、SOP、．SCOD、NH+4-NSOP14．Lowry-Folin．-COD1.501.0715．pHDO/pHMulti340iWTWGermany．VFAsAgilent7890A0.9mL1.5mL0.1mL3%H3PO4pH．N2FIDDB-WAXETＲ30m×1μm×0.53mm220250℃80℃1min20℃/min160℃1min5℃/min180℃1min．2μLVFAs、、、COD1.07、1.51、1.822.0416．1．48、pH4.010.0－20℃FastDNASpinkitforsoilBIO101systemUSADNANanodropSpectrophotometerND-1000ThermoFisherScientificUSADNADNA－20℃．IlluminaMisepDNAPCＲPCＲIlluminaPEIllumina．22．1pHTVFApH．pHTVFATotalVolatileFattyAciol2．TVFA12.6mgCOD/L．TVFATVFA7-8．2pH．4TVFApH8.09.02720.73157.8mgCOD/L．TVFA24．8pH10.0TVFA4156.4mgCOD/LpH11.0TVFATVFApH10.0pH10.0．pHTVFA374No．3pH2pHTVFAFig．2EffectofpHvaluesontotalproductionofVFATVFA12pH11.0pH10.013TVFA17．pH10.0．2．2pHVFAsVFAs18．pHpH10.0VFAs3．VFAs2～5、、、、．82%．3pH10．0VFAsFig．3PercentageofindividualVFAspeciesintheVFAsatthepHof10．0474Vol．268、、、、、1852.3、503.7、397.1、150.7、783.4、3.4mgCOD/LTVFA52.0%、14.0%、10.0%、4.0%、19.8%、0.2%．50%1619．VFAs、、β20．51221．1322．．2．3pHSCODSCOD4SCODTVFApH10.011.0SCOD23．4pHSCODFig．4EffectofpHvaluesonproductionofSCODMaillardＲeaction24．“”．pH．．pH11.0SCOD．8pH11.0pH10.0SCODpH10.0．pH10.08SCOD8842.5mg/L．574No．3pH2．4pH、．VFAsVFAs．pH5．EPSEPSpHEPSEPS25．83～4．26．5pHFig．5EffectofpHvaluesonproductionofproteinandcarbohydrate2．5pH6pH．．pH9.0～11.04．674Vol．268～10d50mg/L．．．pH8．0pHpH8pH8.0．NH3+H2ONH+4+OH－．pHpH．．pH6.0552.2mg/L．6pHFig．6EffectofpHvaluesonproductionofNH+4-N7pHFig．7EffectofpHvaluesonproductionofSOP2．6pH7pH．774No．3pH．2．pH12．427．2．7、8pH4.0pH10.0．OTUOperationalTaxonomicUnits97%OTU．1%others82．ProteobacteriaActinobacteria、Bacteroidetes、SaccharibacteriaFirmicutesProteobacteria59.9%Actinobacteria10.4%、Bacteroidetes8.8%、Firmicutes5.2%Saccharibacteria4.9%．pH4.0Actinobacteria、ProteobacteriaFirmicutes321.6%、38.6%35.9%．pH10.0FirmicutesFirmicutes70.9%Proteobacteria19.3%Actinobacteria6.1%Chloroflexi2.8%FirmicutesClostridia63.4%．8Fig．8ＲelativeabundanceatphylumlevelpH4.010.0FirmicutesChloroflexi．Firmicutes、、、、28．ClostridiaFirmicutes874Vol．262%Table2Taxonomicclassficationofpyrosequencesatgenuslevelunit%GenusClassPhylumpH=4pH=10ZoogloeaBetaproteobacteriaProteobacteria20．64Candidatus_AccumulibacterBetaproteobacteriaProteobacteria4．9Candidatus_MicrothrixActinobacteriaActinobacteria3．81．67TetrasphaeraActinobacteriaActinobacteria3．41DechloromonasBetaproteobacteriaProteobacteria3．25Comamonadaceae_unclassifiedBetaproteobacteriaProteobacteria2．83Mle1-27_norankBetaproteobacteriaProteobacteria2．83Saprospiraceae_unculturedSphingobacteriiaBacteroidetes2．76Saccharibacteria_noranknorankSaccharibacteria2．72Saccharibacteria_unclassifiednorankSaccharibacteria2．22HaliangiumDeltaproteobacteriaProteobacteria1．97OttowiaBetaproteobacteriaProteobacteria1．76TrichococcusBacilliFirmicutes1．691．281．16NS9_marine_group_norankFlavobacteriiaBacteroidetes1．18DokdonellaGammaproteobacteriaProteobacteria1．14FerruginibacterSphingobacteriiaBacteroidetes1．09Nitrosomonadaceae_unculturedBetaproteobacteriaProteobacteria1．03AcidocellaActinobacteriaActinobacteria26．83PropionibacteriumAlphaproteobacteriaProteobacteria20．44Peptostreptococcaceae_unclassifiedClostridiaFirmicutes16．051．34BacillusBacilliFirmicutes5．45Clostridium_sensu_stricto_1ClostridiaFirmicutes4．73Enterobacteriaceae_unclassifiedGammaproteobacteriaProteobacteria2．74IntestinibacterClostridiaFirmicutes2．25TuricibacterErysipelotrichiaFirmicutes2．25BurkholderiaBetaproteobacteriaProteobacteria1．29Family_XI_unculturedClostridiaFirmicutes20．2AcetoanaerobiumClostridiaFirmicutes18．18PseudomonasGammaproteobacteriaProteobacteria17．23AlkaliphilusClostridiaFirmicutes10．13ProteiniclasticumClostridiaFirmicutes5．36BrassicibacterClostridiaFirmicutes3．21JG30-KF-CM45_norankThermomicrobiaChloroflexi2．44GarciellaClostridiaFirmicutes2．21GottschalkiaClostridiaFirmicutes1．38Family_XIV_unculturedClostridiaFirmicutes1．37ErysipelothrixErysipelotrichiaFirmicutes1．34CorynebacteriumErysipelotrichiaFirmicutes1．2129．Chloroflexi30．pH4.010.0ActinobacteriaProteobacteria．Actinobacteriaβ-Proteobacteria31Bacteroidetes32．Bacillus335.45%974No．3pHpH10.0．、．31VFAs．VFAs、SCOD、pH10.0．TVFAsSCOD47%SCOD30%SCOD9%23～4．．．3、．pH4.010.0ProteobacteriaFirmicutes．．1KubaTVanLoosdrechtMHeijnenJJ．PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesy