SBBR系统反硝化聚磷菌的分离及其鉴定李亚峰

20119275JournalofShenyangJianzhuUniversityNaturalScienceSep．2011Vol．27No．52011－02－282009－k7－92010－k7－21960—．2095－1922201105－0952－05SBBR11121.1101682.110142SBBR．SBR/NO－3．32#、3#5#75.82%、78.26%62.48%．SBBR．X703AStudyonSeparationandIdentificationofDenitrifyingPhosphorus-RemovingBacteriainSBBRLIYafeng1HAOYing1ZHANGXiaoning1JINYanqiu21.SchoolofMunicipalandEnvironmentalEngineeringShenyangJianzhuUniversityShenyangChina1101682.SchoolofEnvironmentalandBiologicalEngineeringShenyangHuagongUniversityShenyangChina110142AbstractThestudyaimedtoprovetheabundantexistenceofphosphorus-accumulatinganddenitrifyingstrainsandtoinvestigatetheirdenitrifyingandphosphorusremovingperformancesinSBBRsystem．AddingpolyurethanecarriersinSBRtheexperimentusednitrateaselectronicreceptorstostudydenitrifyingphos-phatationunderanaerobic/anoxicconditions．Theactivatedsludgewasextractedinstabilizationcondition．Theexperimentuseddilutioncoatingmethodandflatcrossedmethodtoseparatethedenitrifyingphosphorusremovingbacteriaandalsousedmetachromaticgranulesdyeingmethodandnitratereducingtesttoidentifythescreeningbacteria．Theresultsshowedthatthreestrainswereextractedwhichhadbothdenitrificationandphosphorusremovingefficacy．TheyallhadmetachromaticgranulesandtheGram'sdyeingwasallneg-ative．Thenitrateremovalratesof2#3#and5#strainswererespectively75.82%78.26%and62.48%．LargeamountofphosphorusremovingbacteriaexistintheSBBRsystemandtheyperformgooddenitrifyingandphosphorusremovingeffect．Keywordsdenitrifyingphosphorusremovingbacteriaflatcrossedsequencingbacthbiofilmreactormeta-chromaticgranulesdyeingnitratereduction27SBBR9531．Kuba21994———DPBDenitrifyingPhosphorusremo-vingBacteriaPAOsO2NO－XPHB3．COD4－7CODCO2CO28．、9－10．1112．SBBR1314．SBBR．．、SBBR15．11.11.1.1①、、、、②、③、④、、、．1.1.2①20g20g2g2g0.5g1000mLpH7.2～7.4．②NO－21g2.42g1g1g20g1000mL．1.1.3①0.15g0.2g1mL95%2mL100mL2g3g300mL．②95%14．1.2SBBRSBBR16．．．．22.1SBBR10mL250mL90mL10－120min．10－11mL9mL10－29542710－21mL9mL10－52100.5mL40℃．．10、30℃3d、．510－410－1、10－210－310－510－410－461．110－4Table1Growthcharacteristicofthecolonyindiluentplatingmedium1#、、、2#、、、3#、、、4#、、5#、、、6#、、2.2Albert142.2.1ATP．．．．31．12#、3#5#Fig.1Metachromaticgranulesdyeingresultof2#3#and5#strainsPoly－P2#、3#5#．2.2.23．2#、3#5#．．．322．27SBBR95522#、3#5#Fig.2Gramstaindyeingresultsof2#3#and5#strains22#、3#、5#Table2Dyeingresultsof2#3#and5#strains2#3#5#2.31～7d17．8mL20min．30℃18．7d．31#、2#3#2#、3#5#．32#、3#5#Fig.3Nitratereducingtestresultsof2#3#and5#strains33．3．2#、3#5#333、57d123．3Table3Removaleffectofnitratereducingtest/%3d5d7d2#49.1567.8375.823#55.7170.5078.265#30.6246.6162.4831SBBR6．22#、3#5#．3．7d2#75.82%3#78.26%5#62.48%．1．J．2009253535－540．JiaoZhongzhiLiXiangkunZhangLichengetal．IsolationandcharacteristicofdenitrifyingphosphorusaccumulatingorganismJ．JournalofShenyang95627JianzhuUniversityNaturalScience2009253535－5402KubaTVanLoosdrechtMCMHerjnenJJ．Effectofcyclicoxygenexposureontheactivityofdenitrif-yingphosphorusremovingbacteriaJ．WatSciTech19963433－40．3．SBRJ．200840121927－1931．ShiYu-mingMaFangWangQiangetal．Isola-tionofPAOsandefficiencyofphosphorousaccumu-lationinSBRsystemJ．JournalofHarbinInstituteofTechnology200840121927－19314KubaTVanLoosdrechtMCMHeijnenJJ．Phos-phorusandnitrogenremovalwithminimalCODre-quirementbyintegrationofdenitrifyingdephosphata-tionandnitrificationinatwo-sludgesystemJ．Wat．Res．19963071702－1710．5KubaTSmoldersGVanLoosdrechtMCMetal．Biologicalphosphorusremovalfromwastewaterbyanaerobic-anoxicsequencingbatchreactorJ．Wat．Sci．Tech．1993275/6241－252．6MerzoukiMBernetNDelgenesJPetal．Biologi-caldenitrifyingphosphorusremovalinSBReffectofaddednitrateconcentrationandsludgeretentiontimeJ．Wat．Sci．Tech．2001433191－194．7Kerrn-JespersenJPHenzeM．BiologicalphosphorusuptakeunderanoxicandaerobicconditionJ．Wat．Res．1993274617－624．8．J．2007283517－521．DaiWenchenZhangHanminXiaoJingnietal．EnrichmentofdenitrifyingphosphateaccumulatingorganismsinsequencingbatchmembranebioreactorsJ．EnvironmentalScience2007283517－5219．J．199824368－73．HaoXiaodi．Europeanwaterenvironmentcontrolphosphorusstrategyandwastewaterdephosphoriza-tiontechnologyUpJ．Waterandwastewateren-gineering199824368－7310．D．2008．ChenLei．StudyonisolationandcharacteristicofdenitrifyingphophateaccumulatingbacteriaD．JiNanSchoolofenvironmentalscienceandengineer-inginShandonguniversity2008．11．J．2007286631－635．MaFangWangChunliWangLili．Screeningofdenitrifyingpolyphosphate-accumulatingorganismsandtheirbiologicalcharacteristicsJ．JournalofHarbinEngineeringUniversity2007286631－63512OehmenALemosPCCarvalhoGetal．Advancedinenhancedbiologicalphosphorusremovalfrommi-crotomacroscaleJ．WaterResearch2007412271－2300．13．SBBRJ．20115138－42．LiYafengYaoJingboHaoYingetal．Studyofaddingpolyurethanefoamasmicro-organismsimmo-bilizedcarriersinsequencingbatchbiofilmreactortoremovenitrogenandphosphorusJ．ChineseJournalofEnvironmentalEngineering20115138－4214．M．1990．QiuWenfang．Environmentalmicrobiologytechni-calmanualM．BeijingAcademypress1990．15TsunedaSOhnoTSoejimaKetal．Simultaneousnitrogenandphosphorusremovalusingdenitrifyingphosphate-accumulatingorganismsinasequencingbatchreactorJ．Biochemicaleng