TTC脱氢酶活性测定仪的研制尹军

　　:2003-03-10.　　:　(1954～),,,,.　　＊[95280],200112.:1009-0185(2003)02-0001-04TTC—＊尹　军　付　瑶　荐志远　王翠兰(,　130021):我们研制的脱氢酶活性测定仪是一种在国内外首次开发研制的测定污水处理中污泥活性的专用仪器,它利用光电原理结合微处理系统,将模拟量直接转化为数字量,实现了浓度直读,解决了脱氢酶活性在实际应用中的困难,大大简化了测定程序,其灵敏度高,稳定性和重现性都很理想.采用该测定仪进行检测,可以使脱氢酶活性这一很有价值的指标能够真正用于污水处理工程.:脱氢酶;测定;活性污泥;污水处理;TTC:TU991　　　　　　　　　　:A　　.,.,,,.,,,.,TTC—,.,,,,,,,,.,TTC—,,,.,.TTCTF(μgTF/mL·h),±2%,、、,,,.,,,、,,,.1　　　,.,(H+/e).,Ⅰ(NAD+-)Ⅱ(NAD+-),(H+/e),,(H+/e),.(H+/e).1.,S1,S2,S3;D1,D2,D3S1,S2,S3.　第20卷　第2期2003年6月吉　林　建　筑　工　程　学　院　学　报JournalofJilinArchitecturalandCivilEngineeringInstituteVol.20　No.2Jun.2003　,,(H+/e),(D1,D2,D3),,,.2,3,5—(2,3,5—TriphenylTetrazoliumchloride,TTC),(O2),TTCH+(H+/e),(TriphenylFormazoneTF).:RH2()R+2H()CNNNNCL-+2H　　CNNNN+HCL　　,485nm.,TTC—,,TTCTF(μgTF/mL·h),,,.2　　　TTC—、、、.(1)　,485nm,、,,—.(2)　、、、,6V,5V.(3)　、、A/D、、.,,,.(4)　1cm.4LED,TTC—.3　3.1　　　(1)　()10mL,,10mL(),.(pH=8.4),TTC,Na2SO4.(2)　37℃±1℃,5min,5mL,,.()30min.(3)　,,,.(4)　4,,,5mL,,37℃±1℃10min.,.3.2　　　(1)　30minTTC—,,,.(2)　,,,.(3)　,,(μgTF/L·h).2吉　林　建　筑　工　程　学　院　学　报第20卷4　TTC—4.1　　　:0.5μgTF/mL·h～1000μgTF/mL·h;:0.5μgTF/mL·h.4.2　　　,≤0.022μgTF/mL·h.1.1　(h)(μgTF/mL·h)(μgTF/mL·h)/h1062.0021862.40-0.0222　　　　　1020.0022419.900.0042　　　　　4.3　、　　,(μgTF/mL·h),、.,≤2%,≤1.081,、.、23.2　　　　20406080100120　　　20406080100120119.9040.8062.0081.55101.85120.97420.1940.8562.0082.45103.00122.35219.9040.7059.8081.9099.90120.85520.0040.6861.3580.30102.20119.70320.1941.1062.4083.00102.63121.85620.3041.0561.0582.45101.85121.203　CEER(%)SRSDCEER(%)SRSD20.080.080.40.1700.85481.941.942.00.9470.64140.940.942.00.3470.848101.901.901.01.0811.06161.431.432.00.9401.530121.091.900.70.8690.7184.4　　　,,,4.4　　　(μgTF/mL·h)　　(μgTF/mL·h)11999.11.20216.3552000.05.16185.9522000.01.225.2062000.05.16107.5332000.03.02163.4872000.05.16115.2442000.05.16173.285　　　..,,,.,,,,,TTCTF(μgTF/mL·h),,,3　第2期尹　军,付　瑶,荐志远,王翠兰:TTC—脱氢酶活性测定仪的研制,,,,,,,、、、.,.　　　　　[1]　,　.TTC-〔J〕.,1996,16(4).　　[2]　　,.TTC〔J〕.,1995,11(4).　　[3]　,　.〔J〕.,1992,8(1).　　[4]　,　.〔M〕.:,1989.　　[5]　　.〔J〕.,2000,16(10).　　[6]　　,.〔J〕.,1996,17(1).　　[7]　,.()〔M〕.:,1997.TheDevelopofTheInstrumentforMeasuringTTC-DehydrogenaseActivityYINJun,FUYao,JIANZhi-yuan,WANGCui-lang(DepartmentofEnvironmentalEngineering,JilinArchitecturalandCivilEngineeringInstitute,Changchun,130021)Abstract:Thisarticlediscussedhowtousetheinstrumentformeasuringdehydrogenaseactivity.Theinstrumentthatisusedtomeasuretheactivityofactivatedsludgeinthewastewatertreatmentwererecentlystudiedandde-velop,itwhichadoptsnewphotoelectrictheorywithminiprocessingsysem,andtransformsimitativequantityintodigitalamount.Thereforeitsolvespracticaldifficultiesformeasuringdehydrogenaseactivity,greatlysimplifiestheprograminthecourseofmeasuring,andithashighsensitivity,stublizationandrecurrene.Asaresultthevaluableindex(dehyeogenaseactivity)canbereollyputintouseforwaster-watertreatmentengineering.Keywords:Dehydrogenase;measurement;activatedsludge;wastewatertreafment;TTC4吉　林　建　筑　工　程　学　院　学　报第20卷