UASB反应器培养厌氧氨氧化菌的试验研究彭绪亚

UASB400045UASBC/N。1130d150mg/L、TN0．36kg/m3·dTN80%2200d340mg/LTN0．80kg/m3·dTN75%～85%。12NH+4－NNO－2－NNO－3－N1∶1．1～1．2∶0．25～0．451∶1．1～1．2∶0．30～0．40pH。。UASBX703A1000－4602201111－0020－05StudyonCultivationofAnammoxBacteriainUASBReactorPENGXu-yaLIZhi-yangWANGLuHEQing-mingHONGJun-huaKeyLaboratoryofThreeGorgesReservoirRegion’sEco-Environment＜MinistryofEducation＞ChongqingUniversityChongqing400045ChinaAbstractInordertoprovideAnammoxsludgeforadvancedtreatmentofpoultrymanurewithlowC/NdifferentconcentrationsofanaerobicsludgewereinoculatedinUASBreactortocultivateAnammoxbacteria．TheexperimentalresultsshowthatwheninfluentconcentrationsofNH+4－NandNO－2－Nareall150mg/LandTNloadis0．36kg/m3·dtheremovalrateofTNisabove80%inNo．1reactorwithlowsludgeconcentrationafteroperationof130d．WhentheinfluentconcentrationsofNH+4－NandNO－2－Nareall340mg/LandTNloadis0．80kg/m3·dtheremovalrateofTNisbetween75%to85%inNo．2reactorwithhighsludgeconcentrationafteroperationof200d．TheratiosamongNH+4－NremovalNO－2－NremovalandNO－3－Nproductionare1∶1．1to1．2∶0．25to0．45and1∶1．1to1．2∶0．30to0．40respectivelyduringthestableoperationperiodofNo．1andNo．2reactors．TheeffluentpHvalueishigherthantheinfluentpHvalue．Itisobviousthatthereactorwithhigherconcentrationofanaerobicsludgehasstrongerresistancetoimpactloadanditismoreadvanta-geoustocultivationofAnammoxsludge．KeywordsAnammoxUASBreactornitrogenremovalSEM2009ZX07104－002－04CSTC2008AB7135·02·271120116CHINAWATER＆WASTEWATERVol．27No．11Jun．2011。、C/N、。1。。2、1、34、5～8UASB、UASB。8．5LUASBUASB。11.191%MLSSMLVSS90．5g/L46．2g/LpH7．308000mg/L。1、2R1、R23L5LMLSS31．94、53．23g/L。、、NH+4－NNO－2－NKH2PO4136mg/LMgSO4·7H2O27．2mg/LCaCl2300mg/LⅠⅡ1mL/L。ⅠEDTA5g/LFeSO45g/LⅡEDTA15g/LZnSO4·7H2O430mg/LCoCl2·6H2O240mg/LMnCl2·4H2O990mg/LCuSO4·5H2O250mg/LNa2MnO4·2H2O220mg/LNiCl2·6H2O190mg/LNa2SeO4·10H2O210mg/LH3BO414mg/L。NaHCO3HClpH。1.2UASB1R1、R28．5L30±1℃120～300mL/min15．0L24h、1～2dpH7．9～8．1。1UASBFig．1SchematicdiagramofUASBreactor1.3NH+4－NNO－3－NNO－2－NN－1－－CODHACHMLSSMLVSSDOpHKYKY－1000。2R1、R2R1150dR2200dR1R2、、、、3。2.1R1、、2。1～4540～80mg/L、0．05～0．10、0．04～0．10、0．09～0．20kg/m3·d。70%·12·．watergasheat．comUASB271165%90%。pH7．3～8．2pHR1。2R1NH+4－N、NO－2－NNO－3－NFig．2VariationofammonianitrogennitriteandnitratecontentinNo．1reactorR2、、3。R280d40～100mg/L、0．05～0．10、0．05～0．120．10～0．22kg/m3·d。20%～40%R2。60%99%。pH7．5～8．3pH。3R2NH+4－N、NO－2－NNO－3－NFig．3VariationofammonianitrogennitriteandnitratecontentinNo．2reactor2.2R145～130、80～150mg/L、0．10～0．18、0．10～0．18、0．20～0．36kg/m3·d90%～98%91%～100%＞80%pH8．1～8．3DO0～0．10mg/L。R280～185、100～270mg/L、0．10～0．32、0．12～0．32、0．22～0．64kg/m3·d96%～100%97%～100%80%～85%·22·2711．watergasheat．compH8．1～8．5DO0～0．05mg/L。2.3R1210mg/L90%50%150～180mg/L30%～40%。DO0．5mg/L。pH7．8～8．2。、10d。R2340mg/L99%98%130～150mg/L75%DO0～0．08mg/LpH7．9～8．3。3d。UASB。2.4R1R2Graaf21∶1．32∶0．269NH+4－NNO－2－NNO－3－N1∶1．1∶0．33。R1NO－2－N/NH+4－N1．0～3．0NO－3－N/NH+4－N0．2～0．4NO－2－N/NH+4－NNO－2－N/NH+4－N1．1～1．2NO－3－N/NH+4－N0．25～0．45NO－2－N/NH+4－N0．5～0．6NO－3－N/NH+4－N0．7～0．9NO－3－N/NH+4－N。R2NO－2－N/NH+4－N0．61．2NO－3－N/NH+4－N0．05～0．30、NO－2－N/NH+4－NNO－3－N/NH+4－NNO－2－N/NH+4－N1．1～1．2NO－3－N/NH+4－N0．30～0．40340mg/LNO－3－N/NH+4－N0．4～0．5。。2.5R1R2。120mL/min300mL/minR1R20．5～1mm1～2mm。Graaf、。3①R1130d150mg/LTN0．36kg/m3·dTN＞80%R2200d340mg/LTN0．80kg/m3·dTN·32·．watergasheat．comUASB271175%～85%。②R1R2NH+4－NNO－2－NNO－3－N1∶1．1～1．2∶0．25～0．451∶1．1～1．2∶0．30～0．40pH。③R1R2。1StrousMHeijnenJJKuenenJG．Thesequencingbatchreactorasapowerfultoolforthestudyofslowlygrowinganaerobicammonium-oxidizingmicroorganismsJ．ApplMicrobiolBiotechnol1998505589－596．2VandeGraafAADeBruijnPRobertsonLAetal．Autotrophicgrowthofanaerobicammonium-oxidizingmi-croorganismsinafluidizedbedreactorJ．Microbiol199614282187－2196．3EgliKFangerUAlvarezPJJetal．Enrichmentandcharacterizationofananammoxbacteriumfromarotatingbiologicalcontactortreatingammonium-richleachateJ．ArchMicrobiol20011753198－207．4SliekersAOThirdKAAbmaWetal．CanonandAna-mmoxinagas-liftreactorJ．FEMSMicrobiolLett20032182339－344．5．J．20042233－7．6．UASBJ．200758112798－2803．7．UASBJ．20032211665－669．8．J．200531199－102．9．J．2008242324－28．1963－。[e-mail]櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵2011－01－0419》《》GB/T19923—2005。1．/J．2008281021－1025．2．－J．2009482－83101．3．J．200420494－96．4．J．200727371－74．5．/J．2010261548－50．6．－J．200632679－81．7．J．200828483－84．8．J．200740274－78．9LeeEKChenVFaneAG．NaturalorganicmatterNOMfoulinginlowpressuremembranefiltration—effectofmembranesandoperationmodesJ．Desalina-tion20082181/3257－270．10．J．200834486－89．1970－、。[e-mail]2011－01－06·42·2711．watergasheat．com