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2007,Vol.24No.7 Chemistry&Bioengineering63 :2007-03-28:章正勇(1983-),男,江苏南京人,硕士研究生,主要从事环境工程技术方面的研究;通讯联系人:安立超,教授。E-mail:zhangzhengyong@163.com。, ,(南京理工大学环境科学与工程系,江苏南京210094) :建立了一种氨氧化细菌的富集培养方法。采用该技术培养30d,氨氧化细菌能够富集达到90%的亚硝酸盐累积率。同时进行了扩大规模的实验,结果也验证了该技术的可行性,为短程硝化—反硝化的实际应用提供了依据。:氨氧化细菌;亚硝酸盐累积率;短程硝化—反硝化:X703.1 Q93-335 :A :1672-5425(2007)07-0063-02 —,O2、,。—。,,。-。[1~3]、pH、、、、。、,,()。、、pH,,,-。1 1.1 (1),5L。,;100W,,;,pH7.5~8.5。1.2 SBR。1 Fig.1 Theschematicdiagramofexperimentalapparatus(g·L-1):(NH4)2SO41.0~1.5;NaHCO32.5;NaCl2.0;K2HPO40.50~0.75;FeSO4·7H2O0.20~0.40;MgSO4·7H2O0.25~0.50;MLSS10。1.3 :5L,24h,(30±1)℃、pH7.5~8.5、0.5mg·L-1。,(NH4)2SO41.0g·L-1,15d,,(NH4)2SO41.5g·L-1,。:,50L,。1.4 DOThermoOrion850A;pHPH-3;:/2007764 [4];N-(1-)-[4];[5]。2 ,,。2.1 2.1.1 (2)2 Fig.2 Thevaryingcurvesofinletconcentration,outletconcentrationandremovalrateofNH3-N2,15d,,52%73%;16d,,3d,50%,,,80%。。2.1.2 (3)3 、Fig.3 Thevaryingcurvesoftheincrementofoutletnitrite,nitrateconcentrationandcumulativerateofnitrite,。3,40%,15d,,61%;16d,,90%,。,。,。,,,。2.2 (4、5)4、5,,90%,。,、,。(下转第67页) :/2007767 ,20min,,30min。30min。3 ,pH4.4、H2O22∶1、30min。,。:[1] .[J].,1998,18(2):4-6.[2] ,,,.N2O,Fe-P[J].,2003,17(5):342-346.[3] ,,.-4-[J].(),2003,41(2):238-240.[4] NiceJA,BewtraJK,TaylorKE,etal.Enzymecatalyzedpoly-merizationandprecipitationofaromaticcompoundsfromwastewater[J].WatSciTech,1992,25(3):157-164.[5] DuranN.Potentialapplicationsofoxidativeenzymesandphenoloxidase-likecompoundsinwastewaterandsoiltreatment.Are-view[J].ApplCatalB,2000,28(2):83-89.StudyontheRemovalofPyrocatecholfromWasteWaterbyHorseradishPeroxidaseZHENGQi,LIZhong-ming(CollegeofChemistryandEnvironmentalEngineering,JianghanUniversity,Wuhan430056,China)Abstract:Treatmentofwastewatercontainingpyrocatecholcatalyzedbyhorseradishperoxidase(HRP)wasstudied.TheresultshowedHRPcouldremovepyrocatecholeffectively.Theoptimumconditionswerede-terminedasfollows:pHvalue4.4,molarratioofH2O2topyrocatechol2∶1,reactiontime30min.Moreover,increasingthedosageofHRPappropriatelycouldshortenreactiontimeobviously.Keywords:horseradishperoxidase;pyrocatechol;enzymecatalyticreaction(上接第64页)3 ,90%,,—。,。:[1] ,,.DOpH[J].,2006,7(1):37-41.[2] ,,.[J].,2004,24(5):618-622.[3] ,,.—[J].,2000,16(2):29-31.[4] 《》.(4)[M].:,2002.[5] ,,.[J].,1999,16(1):41.ExperimentalResearchonEnrichmentTechniqueforAmmoniaOxidizingBacteriaZHANGZheng-yong,CHENXu,ANLi-chao(DepartmentofEnvironmentalScienceandEngineering,NanjingUniversityofScienceandTechnology,Nanjing210094,China)Abstract:Anenrichmenttechniqueforammoniaoxidizingbacteriawasintroducedinthispaper.Witham-moniaoxidizingbacteriaenrichedafter30daysusingtheenrichmenttechnique,thenitriteaccumulationratecouldreach90%.Thelarge-scaleexperimentwasdoneandtheresultvalidatedthefeasibilityoftheenrichmenttechnique,soitprovidedimportantevidenceforpracticalapplicationofshortcutnitrificationanddenitrifica-tion.Keywords:ammoniaoxidizingbacteria;nitriteaccumulationrate;shortcutnitrificationanddenitrification
本文标题:氨氧化细菌富集培养的实验研究章正勇
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