胞外聚合物Ca2及pH值对生物絮凝作用的影响周健

2004,24(4)437~441ChinaEnvironmentalScienceCa2+pH,,,(,400045)(EPS),EPSCa2+pH.,pH7.5,EPS70mg/L,[Ca2+]300mg/L,.,pHEPS[Ca2+]:h=901.70+97.35pH+1.96[Ca2+]+9.08EPS0.11pH⋅[Ca2+]0.91pH⋅EPS0.02[Ca2+]⋅EPS+0.0021pH⋅[Ca2+]⋅EPS.,pH,EPSCa2+.(EPS)Ca2+pHX131A10006923(2004)04043705Effectsofextracelluarpolymericsubstances,Ca2+andpHonbioflocculation.ZHOUJian,LUOYong,LONGTeng-rui,MIAOLi-li(KeyLaboratoryoftheThreeGorgesReservoirRegion'sEco-Environment,ChongqingUniversity,Chongqing400045,China).ChinaEnvironmentalScience,2004,24(4)437~441AbstractApplyingthecationexchangeresinextracelluarpolymericsubstances(EPS)wereextractedfromactivatedsludge,andtheeffectsofEPS,Ca2+andpHonbioflocculationwereinvestigated.Theexperimentresultsshowedthatitwasfavorabletotheformationofbiofloccus,whenpH7.5,EPS70mg/L,[Ca2+]300mg/L.Atthesametime,theregressionequationwasobtainedinorthogonalexperimentforthesyntheseseffectsofpH,EPSandCa2+onbiofloccusformation,theregressionanalysisshowedthattheeffectofpHontheformationofbiofloccuswasmostobvious;EPSandCa2+werethenext.Keywordsbioflocculationextracelluarpolymericsubstances(EPS)Ca2+pHvalue[13],(EPS)[4].[5,6],EPS,.EPS,DNA,10000Dalton.2,,[7].EPS15%[2,8],EPS80%[9].EPSCa2+pH,3.EPS,EPS,Ca2+pH,.11.1EPSEPS,[8].,46000r/min15min;,,4600r/min8h,410000r/min15min,EPS.,DNAEPS,,Lowry,DNA,EPSmgEPS/gVSS.20031229(59838300);(713411001)*,,[e-mail]438241.2pH,22.7100mL,50mLEPS,EPS60mg/L;HClNaOHpH6.0,7.0,7.5,8.0,8.5,9.09.5,CaCl2,Ca2+300mg/L,2min,60min.Z0Z,,h=(Z0Z)/Z0×100%;EPS0EPS,[Ca02+][Ca2+];ΔEPSΔCa2+,ΔEPS=EPS0EPS,ΔCa2+=[Ca02+][Ca2+.,,(mm/min),;60min,,,.1.3,22.4100mL,50mLEPS,EPS50,70,90,130mg/L;HClNaOHpH7.5;CaCl2,Ca2+300mg/L;2min,60min.1.2.1.4,22.7100mL,50mLEPS,EPS60mg/L;HClNaOHpH7.5,CaCl2,Ca2+50,200,400,600,800,1000mg/L,2min,60min.1.2.1.5,pH,EPSCa2+.1.5.1L8(27).1.5.2pH,EPSCa2+,,,1.1Table1LeveloffactorsZ1(pH)Z2(Ca2+)Z3(EPS)Z0j8.540075Δj1.010017+19.55009217.53005822.1pHpH1.pH,(h);ΔCa2+ΔEPS,[Ca2+]EPS.pH7.0,,2.3mL,5.7mm/min;pH7.5,,5.3mL,8.4mm/min;pH8.08.5,,8.2mL,13mm/min;pH9.09.5,,10.3mL,24mm/min.,pH,,.pH,,pHEPS,,,EPS,,,;pH,,,,4Ca2+pH439.1pHFig.1EffectofpHonbioflocculationhΔEPSΔCa2+2.22EPSFig.2EffectofEPSonbioflocculation1EPS2.EPS,(h);ΔCa2+ΔEPS;EPS53mg/L,,2.5mL,7.5mm/min;EPS70mg/L,,6.3mL,10.2mm/min;EPS92,130mg/L,,11.6mL,16.6mm/min.,EPS53mg/L,EPS70mg/L,.,EPS;EPS,EPS,,EPS,,.2.3Ca2+Ca2+3.Ca2+,(h);ΔCa2+ΔEPSh;Ca2+160mg/L,;Ca2+360mg/L,,5.4mL,10.3mm/min;Ca2+510~1100mg/L,,Ca2+1100mg/L,13.3mL,21.3mm/min.Ca2+,Ca2+300mg/L.,Ca2+EPS,Ca2+EPS,;Ca2+,,.[9],Ca2+600mg/L.3Ca2+Fig.3EffectofCa2+onbioflocculation12.4pH,EPS[Ca2+]2.2,ΔEPS,ΔCa2+(mg/L)0204060801005.06.07.08.09.010.0pH020406080100120140160h(%)02040608010030507090110130150EPS(mg/L)020406080100ΔEPS,ΔCa2+(mg/L)h(%)020406080100020040060080010001200050100150200250h(%)[Ca2+](mg/L)ΔEPS,ΔCa2+(mg/L)44024:h=901.70+97.35pH+1.96[Ca2+]+9.08EPS0.11pH⋅[Ca2+]0.91pH⋅EPS0.02[Ca2+]⋅EPS+0.0021pH⋅[Ca2+]⋅EPS(1),pH,[Ca2+],EPSpH[Ca2+],;[Ca2+]EPSpH,[Ca2+],EPS,;pHEPS.2Table2ExperimentresultsandcalculatingoforthogonalregressionX0X1X2X3X1X2X1X3X2X3X1X2X3Y1(h%)Y2(h%)Yi1+Yi2│Yi1Yi2│11111111189.4490.20179.640.7621111111173.6272.60146.221.0231111111180.1281.20161.321.0841111111164.9663.40128.361.5651111111169.0170.20139.211.1961111111163.3962.40125.790.9971111111159.8554.85114.705.0081111111120.7023.7044.403.00Bj=ΣXY1039.6191.44142.08150.1069.7217.3456.4257.34bj=Bj/(8×2)64.9811.978.889.384.361.083.533.58Qj=bjBj2290.61261.71408.1303.8018.79198.95205.5F=Qj/(S/f)880.57485.02541.33116.797.2276.4879.00:S=0.5×Σ(Yi1Yi2)2=6.69;f=N×(M1)=8×(21)=8;h=64.98+11.97X1+8.88X2+9.38X34.36X1X21.08X1X33.53X2X3+3.58X1X2X3X1=(pH8.5)/1.0;X2=([Ca2+]400)/100;X3=(EPS75)/174,,.51,pH[Ca2+]EPS,.,,Ca2+EPS.4,,.15.6mL,23.1mm/min.4(×400)Fig.4SSofsewagewater×Fig.5Floccusformedinexperiment14Ca2+pH441,pH,EPS[Ca2+],.,pHCa2+,,EPS.,.33.1pH,EPSCa2+pH7.5,EPS70mg/L,[Ca2+]300mg/L3.2h=901.70+97.35pH+1.96[Ca2+]+9.08EPS0.11pH⋅[Ca2+]0.91pH⋅EPS0.02[Ca2+]⋅EPS+0.0021pH⋅[Ca2+]⋅EPSEPS[Ca2+][1]ErikssonL,AlmB.Studyofflocculationmechanismsbyobservingeffectsofacomplexingagentonactivatedsludgeproperties[J].WaterSci.Technol.,1991,24(7):2128.[2]UrbainV,BlockJC,ManemJ.Bioflocculationinactivatedsludge:aanalyticapproach[J].WaterRes.,1993,27(5):829838.[3]PavoniJL,TenneyMW,EchelbergerJrWF.Bacterialextracellularpolymersandbiologicalflocculation[J].WaterPollut.ControlFed.,1972,44(3):414431.[4]DavidCSobecka,MatthewJHigginsb.Examinationofthreetheoriesformechanismsofcation-inducedbioflocculation[J].WaterRes.,2002,36(2):527538.[5]MelanieJBrown,LesterJN.Metalremovalinactivatedsludge:theroleofbacterialextracellularpolymers[J].WaterRes.,1979,13(2):817837.[6]FlemmingMC,WingenderJ.Relevanceofmicrobialextracellularpolymericsubstances(EPS)Part:structualandecologicalaspects[J].WaterSci.Technol.,2001,43(6):18.[7]MorganJW,ForsterCF,EvisonL.Acomparativestudyofthenatureofbiopolymersextractedfromanaerobicandaviatedsludge[J].WaterRes.,1990,24(6):743753.[8]BoFrolund,RikkePalmgren,KristianKeiding,etal.Extractionofextracellularpolymericsubstancesfromactivatedsludgeusingacationexchangeresin[J].WaterRes.,199