被孢霉亚麻酸高产菌株选育

24185~92,2005Mycosystema?-1,21*345500014SM96-TM11,(25mg/L)3Mg2+VB6,TM11:MgSO47H2O1.0g,VB6150mg/L6g/L100g/L1mL/LK2HPO42g/LTM1118.0213g/L10.8128g/LGLA668mg/LQ939.97A1672-6472200501-0085-0092?-gamma-linolenicacid,GLAGLAGLAGLA(1)(2)(3)(4)GLAGLA2002(2002)200211.1MortierellaalpinaSM96(200306)*e-mail:[e-mail]2004-07-282004-08-2586241.21.2.1PDA200g30min20g20g1000mLPH12130min1.2.2PDA1.2.3GY10g20gPH6.01.2.4PDA+1.2.5(/)FeSO47H2O10.0CaCl22H2O1.2ZnSO47H2O5CuSO45H2O0.2MnSO44H2O1.0(1997)1.3267d310mL100mL500mL261503d10mL50mL250mL261504d1.430cm15W19990.3%0.4%0.5%1.51.6BurdonClark1985150mL60651998/100%19980.15~1.2g0.0002g250mL10mL,25mLHanus2min2030min,15%20mL100mL0.1mol/LNa2S2O31mL/L1%0.1mol/LNa2S2O3=0.1mol/LNa2S2O3GLA-(CHCl3-KOH-CH3OH)20010.2g4mLCHCl32mL0.5mol/LKOH-CH3OH2min2415min2min3.6mL1minCHCl3HP-6890plus2m$3mm8%(W/W)(DEGS)80-100ChromosorbWN230mL/min30mL/min300mL/min280280160~2084/min2081?-8713min22.1SM96(1)SM9630s25.1%7.6%30s1SM96Table1SelectionofthebestdosageofUVTime15S30S1min2min/Totalstrains(strains/plate)3328125(Livability30.2%25.1%10.5%4.6%Positivemutaterate3.6%7.6%3.2%1.7%SM962SM96SM962SM96Table2SelectionofthebestdosageofLiClDosage0.03%0.04%0.05%0.06%/Totalstrains(strains/plate)241770Livability21%15%6%0Positivemutaterate1.3%1.2%1.4%0SM9630s(3)SM9630s0.04%15.3%8.5%20003SM9630sTable3SelectionofthebestdosageofUVandLiClDosage0.03%0.04%0.05%0.06%/Totalstrains(strains/plate)231770Livability21.2%15.3%6.7%0%Positivemutaterate4.4%8.5%2.2%0%SM9630s0.04%88242.230S0.04%544SM965Table4ComparisionbetweenSM96andthesixstrainsselectedoutStrainBiomass(g/50mL)Fatcontent(%)(Ivalue)(TotalfatIvalue)SM960.30212.3515.6230.2097TM110.35323.6727.0480.5889TM120.37816.6777.8640.4957TM130.35214.6328.5210.4389TM140.39815.4678.6840.5346TM150.35514.5477.8830.4071SM962.3PDA10(5)5SM96Table5ComparisionbetweenSM96andthesixstrainsselectedafterhavingbeenpropagatedfortengenerationsStrainBiomass(g/50mL)Fatcontent(%)(Ivalue)(TotalfatIvalue)SM960.32212.5525.6230.2273TM110.36123.2526.8440.5744TM120.36717.5327.5310.4846TM130.34215.3128.2970.4345TM140.41215.1568.7150.5441TM150.32515.5627.9380.40152.46SM96Table6GCResultComparisionbetweenSM96andthesixstrainsselectedStrain(g/L)Biomass(g/L)Totalfat(mg/L)Oleicacid(mg/L)LA-(mg/L)GLASM968.0501.0102807925TM119.0252.09852114574TM129.1751.61039710841TM138.5501.3093419730TM1410.3001.97043612970TM158.1251.264297100281?-891TM119.560GLA2TM11640Fig1GCresultofTM119.560:GLAFig2FatgranulesofTM11(640)TM11TM14TM11521mg/L145mg/L-74mg/LSM9686.07%83.54%196%GLATM11196%TM1232.5TM11L25(56)7GLA4GLAMgSO47H2O(A)(D)(C)VB6(B)K2HPO4:KH2PO4(F)(E)A2B3C3D5E1F4[MgSO47H2O(A)1g/LVB6(B)150mg/L(C)6g/L(D)100g/L(E)1mg/LK2HPO4(F)2g/L]TM1118.0213g/L10.8128g/LGLA668mg/LMgSO47H2OGLA1g/LGLAGLAGLAGLAGLA6g/LGLAVB6GLAK2HPO4:KH2PO42:22:0GLAGLAGLA90247L25(56)TheresultandanalysisoforthogonaltrialNumber(g/L)BiomassGLA(mg/L)18.3393128215.0407223316.7200355416.1953321518.2867270616.0593425717.4640374818.266766599.5127811011.73533021115.8027424128.986081130.77071148.5207111157.1600104161.45000170.92731187.454092196.258051203.866723210.59731225.9067762310.48756247.3887362510.0320261054978709369934580184775585952784610237211169121291879061616760010938928461104195725353148681190421119614274187116369151172105169205A2B3C3D5E1F4144234242184158123561202191781692216514571297162181313114171223291051?-913GLAFig3RelationsbetweenGLAproductionandfactorsandtheirlevels33.1,GLAGLA6CoA3.2GLAMgSO47H2O(A)(D)GLAMg2+Mg2+GLASinghetal.19963.3GLAGLA,GLAGLAGLAGLA[REFERENCES]BianSG,ZhangXY,hangL,HuangKX,2001.Afastdeterminationofpolyunsaturatedfattyacidsproducedbymaringfungus.icrobiology.28(1):73~76(inChinese)ClarkG(editorinchief),translatedbyLuHN,FengWH,QiaoBY,WuMY.1985.Stainingprocedures.Beijing:SciencePress.1~196.9224(inChinese)HaiH,ShangDJ,LiQW,2002.Advanceinresearchofgamma-linolenicacidproducedbyfungi.IndustrialMycrobiology,32(4):46~50(inChinese)HeZF,ZhangDQ,1998.ChemistryandDetectingTechnology.Beijing:ChinaLightIndustryPress.1~252(inChinese)ShenP,FanXR,LiGW,1999.MicrobiologyExperiment(ThirdEdition).Beijing:HigherEducationPress.1~234.(inChinese)SinghA,WilsonS,WardOP,996.WorldJMicrobiol&Biotechnol,1996(12):7681WangWW,RenPK,2002.Selectivebreedingofgammalinolenicacidhigh-yieldingstrainofRhizopusarrhiausandstudiesofsolidstatefermentationconditions.Mycosystema,21(1):92~97(inChinese)YuanL,2000.ToselecthighyieldstrainsoftetracyclinebyusingcomplexmutagenesisofLiClandultravioletrays.NingxiaMed,22(6):340~341(inChinese)YuHM,WuSQ,YaoRH,1997.Breedingofa-linolenicacidhigh-yieldingfungalstrain.JournalofSouthChinaUniversityofTechnology(NaturalScience),25(12):60~64(inChinese)ZhuGS,LiuZY,LiangZQLeiBX,2002.Progressinproductionof-3polyunsaturatedfattyacidsbyfungalfermentation.GuizhouScience20(3):59~65(inChinese),,,,2001..,28(1):73~76.,,,2002.-.,32(4):46~50,,1998..:.1~252G.,,,,().1985..:.1~196,,,1999..:.1~234.,,2002..,21(1):92~97.2000..,22(6):340~341,,,1997.-.(),25(12):60~64,,,,2002.-3.,20(3):59~65SELECTIONOFHIGH-YIELDINGSTRAINSOFMORTIERELLAPRODUCINGGAMMA-LINOLENICACIDBYUSEUVANDLiClZHUGuo-Sheng1,