苯系化合物好氧降解菌的驯化和筛选

176169912ENVIR()NMENTALCSIENCEVol.17,No.6I:e.,1996`(,1。。。8,,、、、、、1,3,5.,、625,.,.,,.,〔,6〕,.2、、、、、、1,3,5;,.1Ll,24h,(10min),..(1):2.09/IJ(NH;)25();,0.59/IKHZPO;,0.59/LNaHP();,0.39/IMgSO4,0.29/L;(2):5.0ml/L(21.759/LKZHpO;·HZO,33.49/LNaZHPO;·12H2O,8.59/LKHZP()4,5.09/LNH;CI),3.0ml/LMgSO;(22.59/IJ),1.0ml/LCaC12(36.49/L),1.0ml/LFeCI。(0.259/L),1.0(39.9mg/LMnSO;·HZO,42.8mg/LZnSO4·HZO,34.7mg/IJ(NH;)6Mn:02;·4HZO).2pH7.0..、、、、、1,3,5.LZ3(I、l),lI;l.3,28C,,,.,,,(28C),,,(4C).、l.L3、、..L4(1),:19960329DOI:10.13227/j.hjkx.1996.06.00117l/d/ml/mg·Ll860068006800700170017001700505050505050502525252525,ó,`,JCj,、.ó、、.11ù.1ōr、、、dJóJ91,Jnj、、、、,,`,Q`,ó、、、.、0000000044d,rōJJJfUS、、.、J、.1、,,n`,```.、、、2`、r、:59/L,109/L,59/LNacH7.0;“”(2).24h,,,,.(2)50ml,24h,3ml,10min(8000r/mim),,10min(10ooor/min),3,..1.5:(4℃)ht.15ml,,10min,,10ml,2,15ml..GCgA,,ZmX3mm(i.d),OV-17/Chromosorb101,.:80℃;、100℃;120℃.:250℃;:210℃.2.213,25,2.22、,3,8,14.2.24.4,7,A-1,22/633,’/m/m+++++l4)75l①751②4424311--04①2853ATM。①、,:①BTM。②AMx①A。②BMx①BMx②①Box②A1A21CD.,,,,.,.,,,,,,,,,,,.,,2.43.3X0.71.22.0X0.41.22.2X0.42.22.6X0.82.42.8X0.81.01。3X0.42.03.2X0.82.23.3X0.80.41.0X0。20.50.9X0.20.60.8X020.20.3X0.2O。40.6X0.40.30.5X0.20.41.0X0.40。40,9X0。20.40.7X0.20。41.23.2X0.60.61.0X0.20.8].2X0.30.40.8X0.20.81.20.50.60.8X0.40.60.8X0.4l,01.5X0.60.81.3X0.60。60。8X0.40.81.2X0.51.01.4X0.6++++++AAABBBABABAABBBBBBBBBBl)A,B4/mg·IJ1/mg·Ll/%/mg·IJl/mg.Ll/%255401468100,1`..…OJ4OtJ,Jg``,`ō/rI.……`ónOrù9q`,óJUJJ13UJ」422351016J`boōDI`LLL。。.。./``.n`Oébdjōō,.d14①751①751②44243l104①A154,954。954.954。954954。946。81005.、/mg.Lj/n·IJ1/%343434.0100100100100100100IOC100%.2751①43l,751①242%54%;431275%46%,2(Bacus)。5、100%,00OJOJ34:!8A②BMx①B②Cx①x)②D30.917.7.3BMx①、BMx②C,100%.BxM①BMx②,,,(lEvaac-t~m),.6.4ATMB①、AMTB②、BTMB①、BTMB②F,BTMn②F,44%46%.B1100%.2.336,,,431A1;BTMB②F;BxM①TBMx②;2oBx①BMTB②;DB1.,,,,,.3(1)25、、、、;,13,,100%.(2),..(3),.6./mg·L’/mg·L’4/ATMB①ATM。②BMB①MB②F::.::::::.:::`:252126444611:{:100100PatriekJEeralgradationoftolueenadnmxyleenadntransformatiomofoxylenebydenitrifyingenriehmenteul-tures.Appl.andEnvlron.Mierobiol.,1991,57:450454PatriekJEetalMetabOlitesformedduringanaerobietrans-formationoftolueneandoxyleneandtheirprosedrelation-shiptotheinitialstepsoftoluenemineraliaztion.Appl.andEnvron.Mierob01.,1992,58:496501EdwardsEAetalAnaerobiedradationoftolueneandxy-lenebyaquifermieroorganismsudnersulfaterdeueingeondl-tionds.Appl.andEnviron.Mierobiol.,1992,58:794800HarryRBetalMetaobilebypruetsofanaerobietoluenedegradationbysulfaterdeueingenriehmenteultures.Appl·andEnviron.Mieorbiol.,1992,58:31923195WouterADetaloCmtitioninehemostateulturebetweenPseudomonasstarinsthatusedifferentPathwaysforthedegra-dationoftoluene.APpl.andEnviron.Mierobiol.,1994,60:28582863JangYoungLeeetalAppl.andEnviron.Microbiol.,1995。`1,22112217AbstraCtSHUANJINKGEXUEEnvironmentalSeieneeVol.,17No.6,1996】lationofBaetedaforpgeradatlonBenezneHom`logueunderAeorbicCondltlon.LuJun,WangJusietal。(ReseacrhCenterforEeoEnvironmentalcSienees,ChineseAeademyofiences,eBijing100085):hC,J..cSi.,17(6),1996,PP.14Inodrertosiftforbaeteriaofdegradingbeneznehom-ologueeomudns,25strainsdegardingtoluene,ethylben-ezne,o,mandPxyleneandtrimethylbeneznewereisoaltedbyseleetiveenirehmentformaetivatdesludgesoftrcohemiealadnmuniei1wastewatertreatmentplants.ThestrainsePciesaerabletodegardethebeneznehomofogueeomundsasthesolesoucreofearbonandenergy.TheersultsindieatedthatrOOtofthe25strainshavestrongeapabiiltyofbidoradingbe~hom-ofogueeomudnsunderaerobieeondition.Keywodrs:benzenehomologueeomunds,aeorbiedegradation,baeteria,isolation.A】yofEconomicLoSSfromEeologicalDeteriora-tioninTypkalcEolicalRgeionsandDivisionDistrietsofCha.WangJunasnandCaiXinde(oSuthChinaIn-stituteofEnviornmentalcSienees,NEPA,GuangzhouStudyontheilquideireulationvecloity,or,niematterdegardationkinetiesandbiofilmdetaehmentarteinairliftbiofilmsusnsionre.`torwaseondueted.Theresultsshowedthattheinnereireulationvelcoity15PorPortionaltothereaetorheightandthe0.5werofthesurfieialgasvelcoityintheriser.ThereaetoreanbeteratdeasCSTRwheninnereicrualtionflux15high.Thebioifllndetaehmentarte15Poroprtionaltothefirstpowerofsu-perfieialgasvecloityinirseradnthebioiflmthieknessandtheZ/3werofearierrquantityineraetor,respeetively.Keywords:airliftbiofihnsuspensionraeetor,innereireu-lationvelcoity,organiematterdradationkineties,di-mensionalanalysis,biofilmdetaehentarte.Tw.Pha.eAaeorbclDigestnofWaetrHy.dP~ttdewithDlluteSulphuclrAeid.ZhouYuexietal.(ChineseResearchAeademyofEnvironmentalSeienee,eBijing,100012):hCin.J.Envonzr.Sei.,17(6),1996,PP.1316InthisPaPer,diPhasefermentationofwaterPretreatdewithdilutesulPhurieaeidwasstudied.mentalresultsdemonstratdethatspeclficibogas510655):Ch.J.8.Sci.,17(6),1996.pp.134ilterrkilramfershwaterhyaeinthwaswithmethaneeontentofhiogasabout1%.hyaeinthExpeir-yleldof。btainedEffluentInOrdertofindoutthattheexpressingsituationofvari-ouseeofoigealenvironmentaldeteriorationduringthepr。eessofeconomiedeveloPmentatpresentinChinaandas-sessingcomprehensivelytheecoliealenvironmentalim-Paetwhichwasmadebymankindaetivitles,themethdoofquantitativeandse