丙酮处理酵母菌吸附铅前后红外光谱分析比较张敬华

:2004-07-07:(012409041):(1980-),,,;:(1967-),,,,。:1004-3918(2004)06-0777-03张敬华,　邹卫华,　戴盈盈,　韩润平,　石　杰(,　450052,)　:对丙酮处理酵母菌吸附铅前后的红外光谱进行了分析比较。生物材料的红外光谱图主要由蛋白质的吸收带、碳水化合物的吸收带组成。1652cm-1、1534cm-1、1239cm-1处的吸收峰是蛋白质的三个特征吸收峰。1455cm-1处的吸收峰为CH3和CH2的弯曲振动峰;1397cm-1处中等强度的吸收归属于羧基的对称伸缩振动。1200～940cm-1之间出现的强且复杂的谱带归属于醚和碳水化合物中的C-O伸缩振动。915cm-1、890cm-1处两个弱的吸收峰属于细胞壁中多糖的糖环键的振动。吸附Pb2+后,3375cm-1处的吸收峰紫移至3388cm-1,1398cm-1处的峰红移到1384cm-1,说明羟基和羧基在结合Pb2+起重要作用。丙酮处理酵母菌的主要成份和结构吸附前后保持完整。:丙酮处理处理酵母;吸附;红外光谱:O657.33　　　:A[1,2],,,,K+、Na+、Ca2+、Mg2+、Cl-,、。,[3]。。,[4]。[5,6]。———Pb2+,Pb2+,1391cm-11384cm-1。1　1.1　PE-1710FTIR,;(AR),KNO3(AR.)。1.2　()4.5um,1%,,80℃2,。5.0g100ml,50ml,10。,80℃,。0.2g50mL,200mg/LPb2+20mL,1,,80℃2,。1.3　Pb2+,150mgKBr,,,。2　　　,,。、、、(,N-,1-4)。,。FT-IR1。a、b第22卷　第6期2004年12月　　　　　　　　　　　　河　南　科　学HENAN　SCIENCE　　　　　　　　　　　　Vol.22　No.6Dec.2004DOI:10.13537/j.issn.1004-3918.2004.06.016Pb2+。[4,7],3700～3000cm-1,3375cm-1,(chitin)(RNHCOCH3)N-H。2927cm-1CH2。a—,b—Pb2+1　Fig.1　Fouriertransformedinfraredspectrafromacetone-washedyeast(a)andlead-exposedyeastbiomass(b),1652cm-1Ⅰ,C=O,1534cm-1Ⅱ,N-HC-N,1239cm-1Ⅲ,C-NN-H,P=OC=SC-O,。1455cm-1CH3CH2;1398cm-1。1200～940cm-1C-O。915cm-1、890cm-1。915cm-1β-。890cm-1βC1-H[4]。1ab,Pb2+,。3375cm-13388cm-1,,,。,1398cm-11384cm-1。———。,。ⅠⅡ(1652,1534cm-1),Pb2+,Pb2+。3　　　、,Pb2+,3375cm-13388cm-1,1398cm-11384cm-1。。:[1]　DrakeLR,RaysonGD.Plant-deprivedmaterialsformetalion-selectivebindingandpreconcentration[J].Anal.Chem.,1996,68:22A-27A.[2]　,,,.[J].,2000,63(7):25-28.[3]　,,,.[J].(),2000,32(3):72-75.[4]　AshhenazyR,GottlizbL,YannaiS.Characterizationofacetone-washedyeastbiomassfunctionalgroupsinvolvedlead—778—河　南　科　学　　　　　　　　　　　　226biosorption[J].Biotechnologyandbioengineering,1997,55(1):1-10.[5]　FoustE,VoleskyB.ContributionofsulfonategroupsandalginatetoheavymetalbiosorptionbythedrybiomassofSargassumfluitans[J].Environ.Sci.Technol.,1996,30(1):277-282.[6]　,,,.[J].,2000,20(4):489-491.[7]　SchmittJ,FlemmingHC.FTIR-spectroscopyinmicrobialandmaterialanalysis[J].Int.Biodeterioration&Biodegration,1998,41(1):1-11.Comparisonofinfraredspectrabetweenacetone-washedyeastandlead-exposedyeastbiomassZHANGJing-hua,　ZOUWei-hua,　DAIYing-ying,　HANRun-ping,　SHIJie(DepartmentofChemistry,ZhengzhouUniversity,Zhengzhou450052,China)Abstract:Theacetone-washedyeastandlead-exposedyeastbiomasswereexaminedbyinfraredspectroscopy.TheIRspectroscopyofbiomassismainlycomposedbytheadsorptionofcarbohydrates,protein,etc.Thedominatingbandsnear1652,1534and1239cm-1areassignedtoamideI,amideIIandamideⅢ,thecharacteristicIRabsorptionofproteinwhichcouldbeoneofthesignificantcomponentsofcellwalls.Thepeak1455cm-1isattributabletothebendingstretchingofCH2-andCH3-.StrongandcomplexbandsattributedtoetherandhydroxylC-Ostretchingbetween1200and940cm-1.Twoweakabsorptionpeaksat915and890cm-1canbeattributedtotheglycosidebondsinthepolysaccharidesstructureoftheacetone-washedbiomass.Thechangesinthespectrumofthelead-exposedyeastbiomasswereinthestretchofhydroxylgroups,whichshiftedfrom3374to3388cm-1andinthesymmetricalstretchofthecarboxylategroups,whichshiftedfrom1398to1384cm-1.Thecomponentsandstructureoftheexposed-leadbiomassremainintact.Keywords:acetone-washedyeast;infraredspectroscopy—779—200412