不同pH处理对菹草Potamogeton省略rispusLCr6毒

J．LakeSci．(湖泊科学)，2014，26(4):607-615．jlakes．org．E-mail:[e-mail]2014byJournalofLakeSciencespHPotamogetoncrispusL．Cr6+*黄敏，施国新＊＊，夏海威，吴娟，乔绪强，汪鹏合，陈霖210023Cr6+pH、、、、、ProPAspHCr6+．1pHpH7．0Cr6+2Cr6+pH6．0～8．03Cr6+、pH6．07．04Cr6+pH6．07．0Pro5pH4．05．0PutpH6．07．0SpdSpd+Spm/PutSpmpHpH4．05．0Cr6+．pH、Pro、PAs．pH6．0～7．0Pro、PAsSpd+Spm/Put．Cr6+pHToxicologicaleffectsofCr6+onPotamogetoncrispusL．underdifferentpHvaluesHUANGMinSHIGuoxinXIAHaiweiWUJuanQIAOXuqiangWANGPenghe＆CHENLinJiangsuKeyLabofBiodiversityandBiotechnologyCollegeofLifeScienceNanjingNormalUniversityNanjing210023P．Ｒ．ChinaAbstractSterileseedlingsofPotamogetoncrispuswereculturedbytissueculturetechniquewhichwasusedasexperimentalmate-rial．InthisstudyweinvestigatedcontentsofelementphotosyntheticpigmentsreactiveoxygenspeciesＲOSmalondialdehydeMDAandprolinePropolyaminesPAsaswellasactivitiesofantioxidantenzymesinCr6+-treatedP．crispusunderdiffer-entpHvaluessoastoapproachthephysiologicalandbiochemicalmechanismsofpHvalueinaffectingCr6+toxicityofaquaticplants．Theresultsindicatedthat1AcidicconditionsenhancedtheaccumulationofCrwhilepH7．0treatmenthelpedP．crisp-usmaintainthebalanceofnutrientelements2pH6．07．0and8．0treatmentscouldretardthechlorosisandpromotethebio-synthesisofphotosyntheticpigments3ContentsofＲOSandMDAincreasedwhenthepHwas4．05．0and9．0．ActivitiesofsuperoxidedismutaseandcatalasewereimprovedwhenthepHvaluewas4．0and5．0HoweveractivityofperoxidesdecreasedwiththeincreaseofpHvalue4InresponsetoCr6+stressplantsshowedsharpincreaseinproinecontentwhenthepHwas6．0and7．05AnimprovedlevelofputrescinePutwasnotedinpH4．0and5．0treatmentswhilespermidineSpdandfreeSpd+Spm/PutratioincreasedinpH6．0and7．0treatmentsForspermineSpmcontentitgenerallyincreasedwithpHval-ueanditwaslowerofthepH4．0and5．0treatmentsthanthatofthecontrolgroup．OurresultsdemonstratedthatresistanceofP．crispustoCr6+stresscouldbeenhancedbyreducingtheexcessiveaccumulationofCrpromotingphotosyntheticpigmentsremo-vingＲOSefficientlymaintainingthebalanceofnutrientelementsandkeepinghighlevelsofProPAsaswellasfreeSpd+Spm/PutratiowhenpHvaluewaswithintherangeof6．0-7．0．＊＊＊164320H106．2013-06-192013-09-30．1989～[e-mail][e-mail]．(湖泊科学)，2014，26(4)KeywordsPotamogetoncrispusL．Cr6+stresspHvalueelementcontentprolinepolyamine、、、、、、1．60×104t2．Cr6+3、、4、、ＲOS5、、DNA6、7．pH．pH、DNA、pH8．pH、9-11．pHpH．pHpH12-13．CdpH4．5CdpH8．014．Cd2+pHpH6．015．PAsPut、SpdSpm、、16-19．Cr6+pH、、ＲOSMDA、、Pro、Cr6+．11．1PotamogetoncrispusL．PotamogetonaceaePotamogeton．20124．．1．21．2．1．5%H2O215min、10%NaClO30s61cm．6-BA2．0mg/LIBA0．5mg/LMS．2cm6-BA1．0mg/L．11/10Hongland．16h∶8hL∶D240～300μmol/m2·s25℃∶18℃L∶D．1．2．2Cr6+3．Cr6+0．05mmol/LCr6+1/10HoaglandpH0．05mmol/LCr6+1/10HoaglandpH4．0、5．0、6．0、7．0、8．09．00．1mmol/LHCl0．1mmol/LNaOH．1pH2d1．8d3．Cr6+K2Cr207．1．2．30．6gFWICP-AESLeeman．1．2．40．2gFW80%ThermoGENESYS10470、647663nmLichtenthaler20．黄敏等:不同pH处理对菹草(PotamogetoncrispusL．)Cr6+毒害效应的影响6091．2．5O·－2、H2O2MDAO·－221H2O2H2O2MDA22．1．2．6SOD、PODCATSODNBTNBT50%1UPODOD4701UCAT1μmolH2O21U．1．2．7ProPAsPro23PAsAziz24．1．33±．ExcelP＜0．05P＜0．01．SPSS17．0P＜0．05P＜0．01．P＜0．05P＞0.05．22．1pHCr6+Cr6+0．05mmol/LpHpH＞7．0．Cr6+pH4．05．0．pH4．077．66μg/gFWCr6+1．23pH9．0Cr6+0．34．pHr=－0．9778P＜0．01．Cr6+pH．pHCa、P、Mg、Zn．BpHr=0．9659P＜0．01．Cr6+pH7．0Ca、P、Mg、Zn、BpH6．0pHCa、P、MgpH4．09．01．1pHCr6+Tab．1EffectsofdifferentpHvaluesonelementcontentsinP．crispusunderCr6+stress/μg/gFWCrCaPMgZnBCr6+63．35±3．15bc1004．79±30．32bc1043．64±32．90b187．95±9．80bc22．35±0．58b8．26±0．38dCr6+pH=4．077．66±4．19a660．03±40．82e711．63±32．74e128．34±10．78e19．34±0．69c6．73±0．49eCr6+pH=5．068．81±3．31b884．24±35．93d915．83±53．09c174．05±8．17c23．17±0．65b8．10±0．37deCr6+pH=6．058．67±3．57cd1016．19±53．89b1040．21±32．66b202．91±9．26ab23．90±1．06b8．82±0．40dCr6+pH=7．052．92±3．16d1110．98±40．01a1130．93±49．01a219．70±12．66a26．90±0．90a10．63±0．52cCr6+pH=8．027．13±2．40e922．40±24．09cd850．47±31．85cd173．22±9．80c19．94±0．86c13．82±0．98bCr6+pH=9．021．46±2．92e899．11±56．34d784．68±31．04de151．41±9．00d18．40±0．98c17．13±1．06a2．2pHCr6+Cr6+aChl．a、bChl．b、CarpH1．Chl．apH6．0、7．08．0pH7．0Cr6+1．31pH4．0Cr6+0．81．Chl．bpH6．07．0pH9．0Cr6+0．77．CarpH7．08．0Cr6+CarpH4．0Cr6+610J．LakeSci．(湖泊科学)，2014，26(4)1pHCr6+Fig．1EffectsofdifferentpHvaluesonphotosyntheticpigmentcontentsinP．crispusunderCr6+stress0．79．Cr6+pHCr6+Chl．aP＜0．05．pH4．05．0CarP＜0．01．2．3pHCr6+O·－2、H2O2MDACr6+O·－2、H2O2MDApH2．O·－2H2O2pH4．0、5．09．0MDApH4．0、5．0、8．09．0Cr6+．O·－2pH4．0Cr6+1．16pH8．0Cr6+0．89．H2O2pH5．0Cr6+1．21．pH7．0Cr6+0．81．MDA、pH4．0、7．0Cr6+1．440．81．H2O2pH4．0、5．09．0P＜0．05O·－2pH4．05．0．pHMDACr6+P＜0．05．2pHCr6+O·－2、H2O2MDAFig．2EffectsofdifferentpHvaluesonO·－2generationrateH2O2contentandMDAcontentinP．crispusunderCr6+stress2．4pHCr6+SOD、CATPODCr6+SOD、CATpH3．SODpH9．0Cr6+25．27%pH6．07．0．CATpH4．0Cr6+67．44%pH6．0Cr6+26．23%．Cr6+PODpH．pH9．0PODCr6+20．49%pH5．0Cr6+18．20%．SODpH4．0、8．09．0Cr6+P＜0．05CATpH6．0Cr6+P＜0．01pHPODpH4．05．0黄敏等:不同pH处理对菹草(PotamogetoncrispusL．)Cr6+毒害效应的影响611P＜0．05．3pHCr6+SOD、CATPODFig．3EffectsofdifferentpHvaluesonSODCATandPODactivitiesinP．crispusunderCr6+stress2．5pHCr6+Pro4pHCr6+ProFig．4EffectsofdifferentpHvaluesonProcontentinP．crispus