不同TOCNH4N对厌氧氨氧化脱氮效能的影响王凡

38820178ENVIＲONMENTALSCIENCEVol．38No．8Aug．2017TOC/NH+4-N1212121212*121．2150092．215009SBＲTOCNH+4-N．TOC/NH+4-N1.40.26kg·m3·d－1．TOC/NH+4-N0.40.34kg·m3·d－1TOC/NH+4-N0.4．qPCＲPCＲANAMMOX2.9×1011copies·mL－13.15×1010copies·mL－1TOC/NH+4-N1.6NH+4-N．3.0×109copies·mL－1NO－2-NANAMMOXANAMMOX．TOC/NH+4-NPCＲX703.1A0250-3301201708-3415-07DOI10．13227/j．hjkx．2017011852017-01-212017-02-242016YFC040110351478284514083871992～[e-mail]*[e-mail]oNH+4-NＲatiosonNitrogenＲemovalEfficiencyintheANAMMOXProcessWANGFan12LIUKai12LINXing12ZHOUZheng12LIXiang12*HUANGYong121．SchoolofEnvironmentalScienceandEngineeringSuzhouUniversityofScienceandTechnologySuzhou215009China2．InstituteofEnvironmentalBiotechnologySuzhouUniversityofScienceandTechnologySuzhou215009ChinaAbstractTheeffectoftheTOCtoNH+4-NratioonnitrogenremovalrateinanANAMMOXsequencingbatchreactorwasstudiedfortheshorttermandlongterm．Short-termresultsshowedthemaximumTOC/NH+4-NthatANAMMOXcouldaffordwas1.4．Atthesametimetherateofnitrogenremovalwas0.26kg·m3·d－1．Themaximumnitrogenremovalrateof0.34kg·m3·d－1wasobtainedwhenTOC/NH+4-Nwaslessthan0.4inthelong-termexperiment．WhereasthenitrogenremovalrateofthereactordecreasedcontinuouslywhenTOC/NH+4-Nexceeded0.4anditwasdifficulttorecovertheactivityoftheANAMMOXbacteriaintheshortterm．qPCＲresultsshowedthattheamountofANAMMOXbacteriadroppedfrom2.9×1011copies·mL－1to3.15×1010copies·mL－1withtheincreaseoforganics．WhenTOC/NH+4-Nexceeded1.6NH+4-NcouldhardlyberemovedbytheANAMMOXbacteriawhichwereseverelyinhibited．Thenumberofdenitrifyingbacteriawas3.0×109copies·mL－1meanwhilemostoftheNO－2-Ninthereactorwasremovedviadenitrification．KeywordsANAMMOXdenitrificationorganicmatterTOC/NH+4-NquantitativePCＲanaerobicammoniumoxidationANAMMOXNH+4-N．、1～3．ANAMMOX11d45ANAMMOXANAMMOX．Chamchoi6COD＞300mg·L－1ANAMMOX．Molinuevo7USABANAMMOXCOD＞112mg·L－1ANAMMOX．8ANAMMOXUASB1∶138NH+4-N、NO－2-N95.4%、99.3%94.3%．COD9～11TOC12TOCNH+4-NANAMMOX．mTOC/mNH+4-NC/N．11.1ANAMMOX250mL．800mL．35℃100r·min－1．1.2ANAMMOX2.5mmMLVSS/MLSS0.58．mg·L－1NH4Cl100NaNO2130MgCl2300CaCl2100NaHCO31000KH2PO450KHCO3200．Ⅰ、Ⅱ1.0mL·L－1、1.25mL·L－1．Ⅰg·L－1FeSO45EDTA5Ⅱg·L－1EDTA5CuSO4·5H2O0.25ZnSO4·7H2O0.043MnCl2·4H2O0.99CoCl2·6H2O0.24H3BO40.014NaMoO4·2H2O0.22NaSeO4·10H2O0.2．1.313．NH+4-NNO－2-NN-1--NO－3-NMLVSS/MLSSpHpHS-3ETOCICMultiN/C3100．1.41.4.14250mL0.75gANAMMOX．SBＲ250mL．20h5min19.75h5min5min．NO－2-N/NH+4-N1.31～4C/N0.8、1.2、1.6、2.4C/N．1.4.22800mL2gANAMMOX．SBＲ800mL．12h5min11.75h5min5min．NO－2-N/NH+4-N1.3C/N0.4、0.8、1.2、1.6、2.0．1.5DNAC/N0.8、1.2、2.0．DNADNA．DNA5μL1%．－20℃．1.6PCＲANAMMOX、AOB、DB、TBPCＲ、1．20μL0.8μL0.8μL2μLDNA0.4μLＲOXⅡ2μLDntp10μLSYBＲPremixExTaqⅡ6μL．3．DNAPCＲDNA0.8%．PCＲABI7500Ｒ20.995．22.111C/N0.85NO－2-N31.61mg·L－15mg·L－1NO－3-N2mg·L－115mg·L－1NH+4-N61438TOC/NH+4-N1Ｒeal-timePCＲTable1PrimersandparametersofＲeal-timePCＲANAMMOXAmx809FAmx1066ＲGCCGTAAACGATGGGCACTAACGTCTCACGACACGAGCTG95℃2minutes95℃15s58℃30s72℃30s4014AOBamoAFamoAＲGGGGTTTCTACTGGTGGTCCCCTCKGSAAAGCCTTCTTC95℃5minutes95℃30s58℃30s72℃30s4015DBNirsFNirsＲGTSAACGTSAAGGAＲACSGGGASTTCGGＲTGSGTCTTGA95℃2minutes95℃15s62℃30s72℃30s4016TBBac16FBac16ＲCCTACGGGAGGCAGCAGTTACCGCGGCTGCTGGCAC95℃5minutes95℃30s57℃30s72℃30s40171C/NFig．1NitrogenremovalratesunderdifferentCtoNratiosinthereactor0．NO－3-NANAMMOXANAMMOX．NＲＲ0.24kg·m3·d－1．C/N1.0．2C/N1.2．3NO－2-N0NO－3-N1819NO－2-N．6C/N1.4NH+4-N10mg·L－1NO－3-NC/N1.2C/NANAMMOXNO－2-N0ANAMMOXNO－2-N．C/N1.62.03ANAMMOX11NO－2-NNH+4-N197.70%773.84%NＲＲ0.27kg·m3·d－10.24kg·m3·d－1C/N2.0NＲＲ22.46%0.18kg·m3·d－1．C/N2.44714338ANAMMOXNH+4-N97.72%23.45%29TN75%ANAMMOX．2TNC/N1.4TN93.8%．C/N3Fig．3Nitrogenremovalratesofthereactorundertheeffectoforganicmatterinthelongterm1.6TN92.8%1.6TNC/N1.6ANAMMOXC/N1.6C/NANAMMOXNO－2-NNH+4-NTN．2C/NTNFig．2TotalnitrogenremovalefficiencyforthedifferentCtoNratios2.2NＲＲ0.25kg·m3·d－1940mg·L－1TOCC/N0.4．3NH+4-N、NO－2-NNO－3-N．ANAMMOX20120mg·L－1TOC/NH+4-N0.44ANAMMOX21ANAMMOX27.05%．NO－3-NNＲＲ0.34kg·m3·d－1．C/N0.8NH+4-NNO－2-NNO－2-N3ANAMMOXC/NNO－2-N．AOBNH+4-NNO－2-N．ANAMMOXNO－3-NNO－2-NNO－2-N．C/N1.2NH+4-NC/NANAMMOX5.9%NH+4-N．pH7.1～7.58.3～8.7ANAMMOXpH22FA26.03mg·L－1Waki23FAFA81438TOC/NH+4-NANAMMOX24．C/N1.62.0NO－2-NNＲＲ0.30kg·m3·d－199.5%NH+4-NANAMMOX．AOBNH+4-NNO－2-NNO－2-NNO－2-N．83AOBNH+4-NNH+4-NANAMMOX．2.34C/N．4a．4b4cC/N0.8ANAMMOXC/N．——．C/N2.0．5ANAMMOXabc．bANAMMOXb．4C/NFig．4ApparentcharacterofsludgeattheendofdifferentCtoNratiosundertheeffectoforganicmatterinthelongterm5C/N2.0Fig．5SectionalviewofgranularsludgeattheendofCtoNratioof2.02.4C/N0.8、1.2、2.0qPCＲ．C/N2.0qPCＲ．6ANAMMOXC/NC/N2.03.15×1010copies·mL－1DB3.0×109copies·mL－1ANAMMOX2526ANAMMOX108～109copies·mL－1ANAMMOX．TOCANAMMOXTOC200mg·L－1ANAMMOXANAMMOX．67C/N0.8ANAMMOX2.9×1011copies·mL－161.7%1.22.023%2.6%ANAMMOX．C/NAOBAOB5c．C/N2.0AOBANAMMOXAOBANAMMOXAOB914338．6Fig．6Genecopynumbersfordifferentbacteriaatdifferenttimes7Fig．7Percentagesofdifferentbacteriainthetotalbacteria31ANAMMOXC/N1.4NＲＲ0.26kg·m3·d－1．ANAMMOXC/N1.60.17kg·m3·d－1．2ANAMMOXC/N0.4NＲＲ0.34kg·m3·d－1．3qPCＲANAMMOXC/N0.82.9×1011copies·mL－1C/N2.03.15×1010copies·mL－1．ANAMMOXANAMMOX．1MulderAvandeGraafAAＲobertsonLetal．Anaerobic