不同菌源的微生物对邻苯二甲酸二辛酯生物降解性的比较

:1999-09-20:(1964-),,,柴素芬1,曾锋2(1,516015;2,510275):1FS11FS21FS33,FS1FS2FS3pH65~80,70~8070~80;20~35,15~3515~35,:FS1FS2FS3;FS1:;;;:X172:A:1001-6929(2000)05-0011-03TheComparativeStudyofBiodegradabilityofD-in-octylPhthalatebyMicroorganismsfromDifferentSourcesCHAISu-fen1,ZENGFeng2(1.DepartmentofBiology,HuizhouCollege,Huizhou516015,China;2.CollegeofChemistryandChemicalEngineering,ZhongshanUniversity,Guangzhou510275,China)Abstract:ThemicrobialdegradativecharacteristicsofDi-n-octylphthalate(DnOP)wasinvestigatedbystrainsPseudomonasflu-orescensFS1,PseudomonasaeruginasaFS2andBrevibacteriumsp.FS3thatwereisolatedfromtheactivatedsludgeatapetrochem-icalfactoryandapyrochemicalfactory.TheoptimumpHofthe3strainsfordegradingofDnOPis65~80,70~80and70~80;andthetemperatureis20~35,15~35and15~35,respectively.ThedegradationofDnOPbythe3strainscanbede-scribedbythefirst-orderreactionmodel,withtheprimarybiodegradationhal-flivesofDnOP:PseudomonasfluorescensFS1PseudomonasaeruginasaFS2Brevibacteriumsp.FS3.TheexperimentalresultsdemonstratedthatthestrainPseudomonasfluo-rescensFS1hasstrongabilityofdegradingDnOP.Keywords:DnOP;microorganism;biodegradation;degradativekinetics[1],(20%~50%)[2],,,,[3],EPA,,[4~9],,(DnOP)1FS11FS21FS3FS1FS2FS3(FS1,FS2FS3)135ResearchofEnvironmentalSciencesVol.13,No.5,2000DnOP,1111g/LK2HOP43H2O,1g/LNaCl,05g/LNH4NO3,04g/LMgSO47H2O,01g/LCaCl22H2O,001g/LFeCl3,pH7,12130min,DnOP1260mg/L(,,,,,(2-)10mg/L),307d,120,240,3601200mg/L,,1FS1;1FS21FS3()13100mL(3g/L,5g/L,5g/LNaCl,pH7,12130min)12~24h,(10000r/min)5min,,pH7,002mol/LNa2HPO4-NaH2PO43,002mol/LNa2HPO4-NaH2PO4pH71mL20%100mg/L50mL,30,1414120mL,10min,20mL3,,Na2SO4,5mLHP6890142HP6890HP5(30m320m025m),8035/min290;FID,300;260,1L221FS1,FS2FS3DnOP,3pH,DnOP,11FS1,FS2FS3DnOPTable1TheoptimumconditionsofDnOPbiodegradationbythestrainsPseudomonasfluorescensFS1,PseudomonasaeruginasaFS2andBrevibacteriumsp.FS3pH()(%)(r/min)FS16.5~8.020~350.02~4.560~135FS27.0~8.015~350.02~4.560~135FS37.0~8.015~350.02~4.560~13522FS1,FS2FS3DnOP3DnOP,,125,25,50,100,150,200mg/LDnOP,1图1FS1,FS2和FS3对不同浓度DnOP的降解(30)Fig.1DnOPbiodegradationofdifferentconcentrationsbythestrainsPseudomonasfluorescensFS1,PseudomonasaeruginasaFS2andBrevibacteriumsp.FS3(30)12133DnOP,:lnC=-kt+A(1):kDnOP;CDnOP,mg/L;t,d;ADnOP,:t1/2=ln2/k(2)122FS1,FS2FS3DnOPTable2KineticequationsofDnOPbiodegradationbythestrainsPseudomonasfluorescensFS1,PseudomonasaeruginasaFS2andBrevibacteriumsp.FS3DEHP(mg/L)t1/2(d)rFS112.52550100150200lnC=-0.1012t+2.53lnC=-0.0920t+3.22lnC=-0.0920t+3.91lnC=-0.0915t+4.61lnC=-0.0693t+5.01lnC=-0.0523t+5.306.857.537.537.5710.0013.000.97260.96140.92850.96320.98110.9296FS212.52550100150200lnC=-0.0562t+2.53lnC=-0.0560t+3.22lnC=-0.0559t+3.91lnC=-0.0542t+4.61lnC=-0.0455t+5.01lnC=-0.0379t+5.3012.3312.3712.4012.7915.2318.290.93740.92840.96620.92670.93620.9661FS312.52550100150200lnC=-0.0436t+2.53lnC=-0.0430t+3.22lnC=-0.0422t+3.91lnC=-0.0402t+4.61lnC=-0.0386t+5.01lnC=-0.0378t+5.3015.8916.1216.4217.2417.9618.340.96240.91280.92690.92720.95830.92482,DnOP100mg/L,FS1FS2DnOP,:FS1,lnC=-00942t+A;FS2,lnC=-00556t+At1/2:FS1,t1/2=735d;FS2,t1/2=1246dFS3,DnOP150mg/L,,:FS3,lnC=-00415t+A;t1/2=1670dFS1,FS2FS3DnOPt1/2:FS1FS2FS3FS1DnOP3aFS1FS2,FS3FS1pH65~80,20~35;FS2FS3pH70~80,15~35b100mg/L,FS1FS2,:FS1,lnC=-00942t+A;FS2,lnC=-00556t+At1/2:FS1,t1/2=735d,FS2,t1/2=1246dFS3,150mg/L,,:FS3,lnC=-00415t+A;t1/2=1670dFS1,FS2FS3t1/2:FS1FS2FS3FS1:[1][J],1993,1(2):36~47.[2][M]:,1990.268~270[3]CharlesAS,PetersonDR,ParkertonTF,etal.Theeniviron-mentalfateofphthalateesters:aliteraturereview[J].Chemo-sphere,1997,35(4):667~749.[4],,,[J].,1997,17(3):340~345.[5]WangJianlong,LiuPing,ShiHanchang,etal.Biodegradationofphthalicacidesterinsoilbyindigenousandintroducedmicroor-ganisms[J].Chemosphere,1997,35(8):1747~1754[6]ScheunertID,VocjelD,SchmitzerJ,etal.Biomineralizationratesof14C-labelledorganicchemicalsinaerobicandanaerobicsuspendedsoil[J].Chemosphere,1987,16(5):1031~1041.[7]IrvineRL,EarleyJParley,KehrbergerGJ,etal.Bioremedia-tionofsoilscontamintedwithbis-(2-ethylhexyl)phthalate(DEHP)inaSoilSludge-SequencingBatchReactor[J].EnvironProg,1993,12(1):39~44.[8]DaveyMW,PerezKT,SoperAE,etal.Significanceofthesur-facemicrolayertotheenvironmentalfateofDi(2-ethylhexyl)ph-thalatepredictedfrommarinemicrocosms[J].MarChem,1990,31:231~269[9]ChauretC,InNissWE,MayfieldCI.Biotransformationat10ofDI-n-Butylphthalateinsubsurfacemicrocosms[J].GroundWater,1996,34(5):791~794.135,: