不同水力停留时间条件下PCL为碳省略水体硝酸盐的效率及微生物群落分析罗国芝

122Vol．12No．220182ChineseJournalofEnvironmentalEngineeringFeb．2018143205019002017－07－162017－10－191974—。[e-mail]*PCL123*111231．2013062．2013063．201306、。hydraulicretentiontimeHＲTHＲTpolycaprolactonePCL170～197mg·L－1。。HＲT6h8h0.55±0.32g·L·d－11.05±0.33g·L·d－1HＲT4h2h。PCLPCL。62%Proteobacteria62%AcidovoraxAzospiraComamonasDiaphorobacterIgnavibacteriumFrateuriaPCL。X703A1673-9108201802-0572-09DOI10.12030/j．cjee．201707129Nitrateremovalefficiencyandmicrobialcommunityanalysisofpolycaprolac-tone-packedbioreactorswithPCLascarbonsourcetreatingaquaculturewaterunderdifferenthydraulicretentiontimeLUOGuozhi123*HOUZhiwei1GAOJinfang1TANHongxin1231．ShanghaiEngineeringＲesearchCenterofAquacultureShanghaiOceanUniversityShanghai201306China2．KeyLaboratoryofFreshwaterAquaticGeneticＲesourcesMinistryofAgricultureShanghaiOceanUniversityShanghai201306China3．NationalDemonstrationCenterforExperimentalFisheriesScienceEducationMinistryofEducationShanghaiOceanUniversityShanghai201306ChinaAbstractAdenitrificationfixedfilmbiofilterusingpolycaprolactonePCLascarbonsourceandbiofilmcarri-erwasdevelopedtoinvestigatetheefficiencyofremovingnitrate-nitrogenconcentrationof170to197mg·L－1inwaterfromarecirculatingaquaculturesystem．ThehydraulicretentiontimeHＲTincreasedfrom2hto8h．Theremovalratesof1.05±0.33g·L·d－1wasreachedatHＲTof8hand0.55±0.32g·L·d－1wasreachedatHＲTof6hunuseddissolvedorganiccarbonDOCfromPCLdegradationwaslessthan10mg·L－1forallHＲTsandincreasedwithHＲT．ObservationofthePCLsurfaceindicatedthatchainscissionbyhydrolyticdegradationandbiologicalutilizationinthereactorsdidnotchangethechemicalstructureofthePCLsignificantly．TheresultofmicrobialcommunityanalysisshowedthatProteobacteriawasthepredomi-nantphylumintheinoculumwiththerelativeabundancesof62%．AcidovoraxAzospiraComamonasDiapho-robacterIgnavibacteriumandFrateuriawerecapableofbothdegradingPCLanddenitrifyingNO－3-N．Keywordspolycaprolactonesolid-denitrificationhydraulicretentiontimerecirculatingaquaculturenitrate2PCL301。recirculatingaquaculturesystemsＲASs。。、400～500mg·L－12。75mg·L－13。。、4-5。COD/NO－3-N3.0～6.0。COD/NO－3-N1.0～2.02-3。。biodegradablepolymersBDPs56。PCL、PBS-β-PHB7-9。PCLPCLPCLPCL。11.1PCL2～3mm1。1PCLTable1ParametersofPCLgranulars/kg·L－1/g·10min－1/℃/%Esun800C1.12800001～458～608002Table2Parametersofinfluentwaterfromrecirculatingaquaculturesystem/mg·L－1170.04～197.28/mg·L－10.2～2.64/mg·L－10.13～11.05/mg·L－13.86～22.72/mg·L－15.68～8.43pH7.33～7.87CaCO3/mg·L－119.47～153.79/℃23.4～25.81.2Scortumbarcoo28.39kg·m－3WT600-2JYZ1515x、BT100-2JYZ1515x2。180cm、10cm3。750gPCL5.9L。25℃。1.38、6、42h0.71、0.95、1.43、2.85L·h－1。NO－3-N、3375121Fig．1Diagramofreactorsincurrentexperiment43～26、27～42、43～56、57～68。3dNO－3-N、NO－2-N、NH+4-N、TN、DOC、alkalinityALK、、DO、pH。1dPCL。PCL、。1.4NO－3-NN-1--NO－2-NNH+4-N-TN。、DO、pHWTWMulti3430。MultiN/C2100。NO－3-N、TNW=Ci－Ce/Ci×100%1Ｒ=Ci－CeQ/V2WNO－3-N、TN%ＲNO－3-N、TNg·L·d－1CiCeNO－3-NTN、mg·L－1QL·h－1VPCLL5.9L。NO－3-N。1.5PCL2000PCL。PCL35℃。PCLKQ2200EPCLPCL35℃3。L=Wi－We/Wi×100%3LPCL%WiPCLgWePCLg。1.6SEM10、PCLPCLS3400NIIPCL。10PCL2.5%2h。50%、70%、80%、90%、100%10min。PCL。PCLPCLQuanta200FEGHollandPCL。1.7PCL。NicoletNexus470ThermoNicoletCorporationUSAPCL。1.8150mLPCL30mL30min。。4752PCL22.1NO－3-NTN2NO－3-NTN。HＲT。NO－3-NHＲT8hNO－3-N3HＲT6hNO－3-N3HＲT4hHＲT2hNO－3-NHＲT8h6h。2NO－3-NTNFig．2VariationofnitrateNO－3-NandtotalnitrogenTNconcentrationandremovalratesininfluentandeffluentHＲT。CHU10HＲTNO－3-NNO－2-N。GIBEＲT11。3NO－3-NTable3ＲemovalratesofNO－3-NunderdifferentHＲTconditions/h/g·L·d－1/g·L·d－120.240.22～0.2640.060.05～0.0760.550.23～0.8781.050.72～1.38NO－3-NNO－3-NTNHＲTNO－3-N3。HＲT8hNO－3-N181.38g·L·d－11.05±0.33g·L·d－1HＲT6hNO－3-N0.55±0.32g·L·d－1。LI12、WU13PCLNO－3-N0.59～3.80g·L·d－1。NO－3-NHＲT、、NO－3-N。NO－3-N6hHＲT。NO－3-NN2NH+4-N、NO－2-N。NO－3-NTN。TNHＲTNO－3-NNO－3-N。PCLNO－3-N。2.2NO－2-NNH+4-NNO－3-NNH+4-N、NO－2-N57512。3NH+4-N、NO－2-N。3TANNO－2-NFig．3ChangeofconcentrationsoftotalammoniumnitrogenTANandnitritenitrogenNO－2-NininfluentandeffluentNO－2-NNO－2-N。3HＲT8hNO－2-N1mg·L－13HＲTNO－2-NNO－2-N。4HＲTNH+4-NNH+4-N。4Fig．4VariationofdissolvedorganiccarbonDOCininfluentandeffluent2.3DOCPCLPCL12±0.5%。DOC4。HＲT8hDOCDOCHＲT6、42hDOCDOC。14-15PCLDOC15.46～59.11mg·L－1。4HＲTDOCDOC。DOCDOC。DOC、6。PCLDOC。LUO16PCLDOCDOC。8hHＲTNO－3-NDOCHＲTDOC。DOCNO－3-N。2.4PCL530200PCL500010000PCL。PCLPCL。2.5PCLPCL6PCLFTIＲSpectrometer6752PCL5PCLFig．5SEMphotosofPCLandbiofilmsofPCLgranulesbeforeandafterbeingused。PCL1732cm－1CO1169cm－1—C—O—C—2949cm－12866cm－1—CH3444cm－1—OH17。PCL。2.6BDPs、。BOLEY18PCLNO－3-N2465Acidovorax、Pseudomonas、Bre-vundimonas、KocuriaMicrococcus。Acidovorax78%。CHU17454PCLDiaphorobacter、Hydrogenophaga、ＲhodocyclaceaeDesulfovibrioDiaphorobacter19.45%。WU13PCLAlicycliphilus8.36%Diaphorobacter4.59%。PCL1%、、207。6PCLFig．6FourierinfraredscanningofPCLbeforeandafterbeingused99%。ProteobacteriaBacteroidetes63.9%27.4%12。Betaproteobac-teriaβ-30.67%、Deltaproteobacteriaδ-21.19%Flavobacteriia14.66%。Burkholderi27.08%、Bdellovibrionales19.51%、Flavobacteriales14.66%。Comamonadaceae20.75%、Bdellovibrionaceae19.41%Flavobacteriaceae11.81%。1%Bdellovibrio19.4%、Comamonas9.6%、77512768PCLFig．7MainlybacteriacompositionofsurfacebiofilmofP